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BRD4 Antibody (BL-151-6F11)

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Western Blot: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Whole cell lysate (50 ug) from BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] usedat ...read more
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Detection of human BRD4 (red) in formaldelyde-fixed Hep-G2 cells by ICC-IF. Antibody: Rabbit anti-BRD4 recombinant monoclonal ...read more
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11]. (Secondary: HRPconjugated goat anti-rabbit IgG Substrate: DAB.
Flow Cytometry: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Western Blot: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit ...read more
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Section of ovarian carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] used at 1:50. Secondary: DyLight® ...read more
Independent Antibodies: Immunoprecipitation: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Detection of human BRD4 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-BRD4 recombinant monoclonal ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
BL-151-6F11
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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BRD4 Antibody (BL-151-6F11) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Immunogen sequence between amino acids 1312 and 1362
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BRD4
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:25 - 1:250
  • Immunohistochemistry 1:25 - 1:250
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC/IF Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. FLOW Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for BRD4 Antibody (BL-151-6F11)

  • BRD4
  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Background

Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BRD4 Antibody (NBP2-76394) (0)

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Secondary Antibodies

 

Isotype Controls

Additional BRD4 Products

Research Areas for BRD4 Antibody (NBP2-76394)

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Blogs on BRD4.

Epigenetic Control of Autophagy
By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studi...  Read full blog post.

Understanding the relationship between NUT and BET proteins in NMC
NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati...  Read full blog post.

NUT - A Protein Coding Gene
The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating...  Read full blog post.

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Bioinformatics

Gene Symbol BRD4