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BRD4 Antibody (7L7M8)

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Western Blot: BRD4 Antibody (7L7M8) [NBP3-15452] - Western blot analysis of extracts of various cell lines, using BRD4 Rabbit mAb (NBP3-15452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: BRD4 Antibody (7L7M8) [NBP3-15452] - Western blot analysis of extracts of various cell lines, using BRD4 Rabbit mAb (NBP3-15452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunohistochemistry-Paraffin: BRD4 Antibody (7L7M8) [NBP3-15452] - Immunohistochemistry of paraffin-embedded human colon using BRD4 Rabbit mAb (NBP3-15452) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
7L7M8
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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BRD4 Antibody (7L7M8) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human BRD4 (O60885). IIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTT
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BRD4
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for BRD4 Antibody (7L7M8)

  • BRD4
  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Background

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP3-15452
BRD4 Antibody (7L7M8)
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Applications: WB, IHC

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BRD4