Novus Biologicals products are now on bio-techne.com

Biotin Antibody (rBTN/8819) [mFluor Violet 500 SE]

Images

 
Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, Flow, Func, IHC, ICC/IF
Clone
rBTN/8819
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 500 SE

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Biotin Antibody (rBTN/8819) [mFluor Violet 500 SE] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. It is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry-Paraffin
  • In-situ Hybridization
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Biotin Antibody (rBTN/8819) [mFluor Violet 500 SE]

  • Biotin
  • Vitamin B4

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Biotin Antibody (NBP3-27043MFV500) (0)

There are no publications for Biotin Antibody (NBP3-27043MFV500).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Biotin Antibody (NBP3-27043MFV500) (0)

There are no reviews for Biotin Antibody (NBP3-27043MFV500). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Biotin Antibody (NBP3-27043MFV500) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Biotin Antibody (rBTN/8819) [mFluor Violet 500 SE] and receive a gift card or discount.