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Biotin Antibody (rBTN/8819) [DyLight 594]

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Vial of DyLight 594 conjugated antibody. DyLight 594 is optimally excited at 593 nm by the Yellow-Green laser (561 nm) and has an emission maximum of 618 nm.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, Flow, Func, IHC, ICC/IF
Clone
rBTN/8819
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 594

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Biotin Antibody (rBTN/8819) [DyLight 594] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. It is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry-Paraffin
  • In-situ Hybridization
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Biotin Antibody (rBTN/8819) [DyLight 594]

  • Biotin
  • Vitamin B4

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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