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Biotin Antibody (2758A) [Unconjugated]

 
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Western blot shows recombinant Human VEGF 165 aa1-191/Avitag, biotinylated via BirA (AVI293). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Biotin Monoclonal Antibody (Catalog # MAB10915) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for Biotin at approximately various kDa (as indicated). This experiment was conducted under reducing conditions.
Western blot shows recombinant Human VEGF 165 aa1-191/Avitag, biotinylated via BirA (AVI293). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Biotin Monoclonal Antibody (Catalog # MAB10915) followed by ...read more
Biotin was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Biotin Monoclonal Antibody (Catalog # MAB10915) at 3 µg/mL for 1 hour at room temperature followed by ...read more
Simple Western lane view shows lysates of biotinylated mouse IgG, loaded at 0.2 mg/mL. Specific bands were detected for Biotin at various kDa (as indicated) using 20 µg/mL of Rabbit Anti-Biotin Monoclonal Antibody ...read more
Human peripheral blood lymphocytes were stained with Biotinylated Goat Anti-Human CD4 Affinity-purified Polyclonal Antibody (BAF379) followed by (A) Rabbit anti-Biotin Monoclonal Antibody (MAB10915) or (B) Rabbit IgG ...read more
Biotin was detected in immersion fixed HeLa cells using Rabbit Anti-Biotin Monoclonal Antibody (Catalog # MAB10915) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, Simple Western, Flow, IHC, ICC/IF
Clone
2758A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB10915-SP
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Biotin Antibody (2758A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Biotin compound
Specificity
Detects Biotin in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 1-8 ug/mL
  • Immunohistochemistry 1-3 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Biotin Antibody (2758A) [Unconjugated]

  • Biotin
  • Vitamin B4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Biotin Antibody (MAB10915) (0)

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Secondary Antibodies

 

Isotype Controls

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