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beta-Catenin Antibody (196618) [Unconjugated]

Images

 
beta -Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human beta -Catenin Monoclonal Antibody (Catalog # MAB13291) for 3 hours at room temperature. ...read more
beta -Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human beta -Catenin Monoclonal Antibody (Catalog # MAB13291) at 1.7 µg/mL overnight at 4 °C. Tissue was ...read more
Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
196618
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

beta-Catenin Antibody (196618) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human beta -Catenin
aa 2-781
Specificity
Detects human  beta -Catenin in direct ELISAs.
Source
N/A
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
CTNNB1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB13291 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-Catenin Antibody (196618) [Unconjugated]

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Background

Beta-Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as as transcriptional co-activator in the nucleus and is also involved the canonical Wnt signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB13291
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Applications: WB, IHC, ICC/IF

Publications for beta-Catenin Antibody (MAB13291)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(2)
ICC
(2)
IHC
(3)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB13291 Applications Species
S ?en, CJ Lux, R Erber A Potential Role of Semaphorin 3A during Orthodontic Tooth Movement International Journal of Molecular Sciences, 2021-08-02;22(15):. 2021-08-02 [PMID: 34361063] (ICC, Human) ICC Human
GFA de Moraes, E Listik, GZ Justo, CM Vicente, L Toma The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation BMC molecular and cell biology, 2021-05-04;22(1):26. 2021-05-04 [PMID: 33947326] (Flow Cytometry, IHC, Immunoprecipitation, Western Blot, Human) Flow Cytometry, IHC, Immunoprecipitation, Western Blot Human
Nir Drayman, Parthiv Patel, Luke Vistain, Savaş Tay HSV-1 single-cell analysis reveals the activation of anti-viral and developmental programs in distinct sub-populations eLife 5/15/2019 [PMID: 31090537]
A Kasprzak, K Rogacki, A Adamek, K Sterzy?ska, W Przybyszew, A Seraszek-J, C Helak-?apa, P Pyda Tissue expression of ?-catenin and E- and N-cadherins in chronic hepatitis C and hepatocellular carcinoma Arch Med Sci, 2017-01-19;13(6):1269-1280. 2017-01-19 [PMID: 29181057] (Human) Human
Riordan D, Varma S, West R, Brown P Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 2015-07-15;10(7):e0128975. 2015-07-15 [PMID: 26176839] (IHC-P, Human) IHC-P Human
Vicente C, Lima M, Nader H, Toma L SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells. J Exp Clin Cancer Res, 2015-03-14;34(0):25. 2015-03-14 [PMID: 25887999] (Flow Cytometry, IHC, Human) Flow Cytometry, IHC Human
Moore RN, Cherry JF, Mathur V E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells. Stem Cells Dev., 2011-06-01;21(1):30-41. 2011-06-01 [PMID: 21469943] (ICC, Human) ICC Human
Botchkareva NV, Kahn M, Ahluwalia G, Shander D Survivin in the human hair follicle. J. Invest. Dermatol., 2006-08-31;127(2):479-82. 2006-08-31 [PMID: 16946715] (IHC, Human) IHC Human

Reviews for beta-Catenin Antibody (MAB13291) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-Catenin Antibody (MAB13291) (0)

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Secondary Antibodies

 

Isotype Controls

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By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol CTNNB1