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ATPase Antibody [Janelia Fluor® 635]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 635

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-98188AF350
Alexa Fluor 405
NBP2-98188AF405
Alexa Fluor 488
NBP2-98188AF488
Alexa Fluor 532
NBP2-98188AF532
Alexa Fluor 594
NBP2-98188AF594
Alexa Fluor 647
NBP2-98188AF647
Alexa Fluor 700
NBP2-98188AF700
Alexa Fluor 750
NBP2-98188AF750
Allophycocyanin
NBP2-98188APC
Biotin
NBP2-98188B
CoraFluor 1
NBP2-98188CL1
DyLight 350
NBP2-98188UV
DyLight 405
NBP2-98188V
DyLight 488
NBP2-98188G
DyLight 550
NBP2-98188R
DyLight 594
NBP2-98188DL594
DyLight 650
NBP2-98188C
DyLight 680
NBP2-98188FR
DyLight 755
NBP2-98188IR
FITC
NBP2-98188F
HRP
NBP2-98188H
Janelia Fluor 525
NBP2-98188JF525
Janelia Fluor 549
NBP2-98188JF549
Janelia Fluor 585
NBP2-98188JF585
Janelia Fluor 646
NBP2-98188JF646
Janelia Fluor 669
NBP2-98188JF669
PE
NBP2-98188PE
PerCP
NBP2-98188PCP
Unconjugated
NBP2-98188-100ul
mFluor Violet 450 SE
NBP2-98188MFV450
mFluor Violet 500 SE
NBP2-98188MFV500
mFluor Violet 610 SE
NBP2-98188MFV610
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ATPase Antibody [Janelia Fluor® 635] Summary

Immunogen
Produced in rabbits immunized with E. coli-derived Human ATPase fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAH8
Purity
Antigen and protein A Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Antigen and protein A Affinity-purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for ATPase Antibody [Janelia Fluor® 635]

  • ATPase
  • axonemal
  • dynein, axonemal, heavy chain 8
  • dynein, axonemal, heavy polypeptide 8
  • FLJ25850
  • FLJ36115
  • FLJ36334
  • hdhc9

Background

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ATPase Antibody
[Janelia Fluor® ...
NBP2-98188JF635
ATPase Antibody [Janelia Fluor® 635]
Species: Hu
Applications: IHC

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Product General Protocols

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FAQs for ATPase Antibody (NBP2-98188JF635). (Showing 1 - 4 of 4 FAQ).

  1. I have phosphate in my enzyme. What can I do?
    • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
  2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
    • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
  3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
    • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
  4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
    • Yes, only the phosphate will give a signal; pyrophosphate will not.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DNAH8