Would you be able to share the protocol used to produce the WB image for NB110-41486 - ATP13A2 antibody?

Name: ATP13A2 Antibody
Brand: Novus Biologicals
SKU: NB110-41486
Price: 399 USD

Question

Accepted Answer

Please find the specific protocol used for NB110-41486 in Western Blot from this link: Western Blot Protocol

Immunogen	A synthetic peptide made to an internal region of the human ATP13A2 protein (within residues 200-300). [Swiss-Prot# Q9NQ11]
Localization	Membrane
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	ATP13A2
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

ATP13A2 Antibody

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	Tris-Glycine and 0.15M NaCl
Preservative	0.05% Sodium Azide
Concentration	0.75 mg/ml
Purity	Immunogen affinity purified

Publications using NB110-41486	Applications	Species
Gao H, Sun H, Yan N et al. ATP13A2 Declines Zinc-Induced Accumulation of ?-Synuclein in a Parkinson's Disease Model International Journal of Molecular Sciences 2022-07-21 [PMID: 35887392]
Henry AG, Aghamohammadzadeh S, Samaroo H et al. Pathogenic LRRK2 mutations, through increased kinase activity, produce enlarged lysosomes with reduced degradative capacity and increase ATP13A2 expression. Hum. Mol. Genet. 2015-08-06 [PMID: 26251043] (WB, Human) Details: ATP13A2 antibody used for WB on human post-mortem samples from prefrontal cortex of control, LRRK2 G2019S carriers and idiopathic PD patients (Figure 7).	WB	Human
Dehay B, Ramirez A, Martinez-Vicente M, Perier C, Canron MH, Doudnikoff E, Vital A, Vila M, Klein C, Bezard E. Loss of P-type ATPase ATP13A2/PARK9 function induces general lysosomal deficiency and leads to Parkinson disease neurodegeneration. Proc Natl Acad Sci U S A. 2012-05-30 [PMID: 22647602] (IF/IHC, WB, Human)	IF/IHC, WB	Human