Reactivity | HuSpecies Glossary |
Applications | ELISA |
Suitable Sample Type | Cell Culture Supernatant |
Standard Curve Range | 0.156 - 10 ng/ml |
Sensitivity | 150 pg/ml |
Description | Sandwich ELISA for the quantitative determination of Human Asprosin |
Standard Curve Range | 0.156 - 10 ng/ml |
Sensitivity | 150 pg/ml |
Assay Type | Sandwich ELISA |
Sample Volume | 100 ul |
Gene | FBN1 |
Dilutions |
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Application Notes | 1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (mouse monoclonal antibody) to 2 ug/ml in PBS without carrier protein and use it fresh. 2. Dilute the desired amount of Detection Antibody (DET) (1 mg/ml) (mouse monoclonal antibody) to 1 ug/ml in ELISA Buffer and use it fresh. 3. Reconstitute the Streptavidin-HRP (HRP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted HRP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000). 4. Reconstitute the Standard Protein (STD) (recombinant human Asprosin) with 100 ul ELISA Buffer to obtain a concentration of 10 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 10 ng/ml, 5 ng/ml, 2.5 ng/ml, 1.25 pg/ml, 0.625 ng/ml, 0.3125 ng/ml, 0.15625 ng/ml and 0 ng/ml. |
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Components |
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Research Areas for Asprosin Antibody Pair (NBP2-78856)Find related products by research area.
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