![Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22-[p-Ser22]-Antibody-Western-Blot-NBP1-44072-img0004.jpg "Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | ARHGAP22 [p Ser22] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22. (Uniprot: Q8BL80) |
| Modification | p Ser22 |
| Specificity | This antibody is specific for phosphorylated ARHGAP22 at Serine 22. It also recognizes the S397->A mutation but not the S22->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS22 from other sources has not been determined. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ARHGAP22 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product is tested in Western Blot and ELISA useful for Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract. |
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| Publications |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol |
| Preservative | No Preservative |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - ARHGAP22 [Phospo Ser22] Antibody [NBP1-44072] - Protein Molecular weight: 68 kDa Cell extracts from NIH 3T3cells that were transfected with either a null vector (CTRL), ARHGAP22, ARHGAP22-serine 22 to alanine mutation, ARHGAP22 serine 397 to alanine mutation were electroblotted. The left panel was probed with the NBP1-44072 antibody, while the right panel was probed with the NBP1-44073 antibody, each at 1ug/ml. Each antibody recognizes the unmodified ARHGAP22. NBP1-44072 antibody recognized the S397-A mutation but not the S22-A mutation, while NBP1-44073 antibody recognizes the pS22-A mutation and not the pS397-A mutation. This data confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22-[p-Ser22]-Antibody-Western-Blot-NBP1-44072-img0001.jpg "Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - ARHGAP22 [Phospo Ser22] Antibody [NBP1-44072] - Protein Molecular weight: 68 kDa Cell extracts from NIH 3T3cells that were transfected with either a null vector (CTRL), ARHGAP22, ARHGAP22-serine 22 to alanine mutation, ARHGAP22 serine 397 to alanine mutation were electroblotted. The left panel was probed with the NBP1-44072 antibody, while the right panel was probed with the NBP1-44073 antibody, each at 1ug/ml. Each antibody recognizes the unmodified ARHGAP22. NBP1-44072 antibody recognized the S397-A mutation but not the S22-A mutation, while NBP1-44073 antibody recognizes the pS22-A mutation and not the pS397-A mutation. This data confirms the specificity of each ARHGAP22 phospho specific antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")