Arginase 1/ARG1/liver Arginase Antibody - BSA Free

Arginase 1 (ARG1), also known as liver arginase, is a metalloenzyme that is a member of the ureohydrolase superfamily and arginase family (1). ARG1 is known for its role in the urea cycle in catalyzing the conversion of L-arginine into urea and L-ornithine (1). Arginase has two distinct isoforms, with ARG1 being expressed primarily in the liver and ARG2 in extrahepatic tissues (1). Human ARG1 is synthesized as 322 amino acids (aa) in length with a theoretical molecular weight of 35 kDa (1,2). Three ARG1 monomers can form a highly active homotrimer of 105 kDa (1). A key structural feature of the arginase protein is the binuclear magnesium (Mn2+) ions at its core (1).

Arginase and nitric oxidase synthase (NOS) compete for the same L-arginine substrate, creating a delicate balance between pathways (1). Furthermore, bioavailability of L-arginine and ARG1 expression has been implicated in several pathologies including vascular disease, neuronal disease, cardiovascular disease, immune dysfunction, inflammation, and cancer (1,3-5). For instance, ARG1 functions as a macrophage marker, defining the M2 population, while inducible nitric oxide synthase (iNOS) characterizes the M1 population; impaired M1/M2 polarization and changes in ARG1 expression is observed in diseases such as arteriogenesis, asthma, pulmonary fibrosis, and inflammatory bowel disease (1,3). In humans, arginase deficiency, known as argininemia, is an autosomal recessive metabolic disorder characterized by elevated ammonia (hyperammonemia) levels and arginine accumulation (6). Given that many arginase-associated diseases are characterized by upregulation in expression of ARG1, ARG2, or both, arginase inhibitors are currently being studied as a potential therapeutic approach (1,4).

References

1. S Clemente, G., van Waarde, A., F Antunes, I., Domling, A., & H Elsinga, P. (2020). Arginase as a Potential Biomarker of Disease Progression: A Molecular Imaging Perspective. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21155291

2. Uniprot (P05089)

3. Kieler, M., Hofmann, M., & Schabbauer, G. (2021). More than just protein building blocks: How amino acids and related metabolic pathways fuel macrophage polarization. The FEBS Journal. Advance online publication. https://doi.org/10.1111/febs.15715

4. Shosha, E., Fouda, A. Y., Narayanan, S. P., Caldwell, R. W., & Caldwell, R. B. (2020). Is the Arginase Pathway a Novel Therapeutic Avenue for Diabetic Retinopathy?. Journal of Clinical Medicine. https://doi.org/10.3390/jcm9020425

5. Correale J. (2021). Immunosuppressive Amino-Acid Catabolizing Enzymes in Multiple Sclerosis. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.600428

6. Morales, J. A., & Sticco, K. L. (2020). Arginase Deficiency. In StatPearls. StatPearls Publishing.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using NBP1-32731	Applications	Species
Jiang Y, Liao H, Yan L et al. A Metal-Organic Framework-Incorporated Hydrogel for Delivery of Immunomodulatory Neobavaisoflavone to Promote Cartilage Regeneration in Osteoarthritis ACS applied materials & interfaces 2023-10-11 [PMID: 37769191]
Liu H, Zhang L, Liu Z et al. Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization iScience 2023-09-15 [PMID: 37636061]
Sohn HS, Choi JW, Jhun J Et al. Tolerogenic nanoparticles induce type II collagen-specific regulatory T cells and ameliorate osteoarthritis Sci Adv 2022-11-25 [PMID: 36427299] (IF/IHC, Mouse) Details: Citation using the FITC version of this antibody.	IF/IHC	Mouse
Kang R, Gamdzyk M, Luo Y et al. Three Days Delayed Recanalization Improved Neurological Function in pMCAO Rats by Increasing M2 Microglia-Possible Involvement of the IL-4R/STAT6/PPAR gamma Pathway Translational stroke research 2022-07-22 [PMID: 35867328] (WB, Rat) Details: Dilution used 1:1001	WB	Rat
Al-Qazazi R, Lima PDA, Prisco SZ Et al. Macrophage-NLRP3 Activation Promotes Right Ventricle Failure in Pulmonary Arterial Hypertension Am J Respir Crit Care Med 2022-06-14 [PMID: 35699679] (ICC/IF, Rat) Details: Citation using the Alexa Fluor 700 version of this antibody.	ICC/IF	Rat
Zangeneh Z, Khamisipour G, Andalib AR Induced overexpression of MARCH-1 in human macrophages altered to M2 phenotype for suppressing inflammation process Iranian journal of basic medical sciences 2022-04-01 [PMID: 35656075] (WB, Human)	WB	Human
Liang W, Zhang Y, Zhou L et al. Zeb1 regulation of wound-healing-induced inflammation in alkali-damaged corneas iScience 2022-04-15 [PMID: 35340433]
He X, Li X, Zhang M et al. Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation Biomaterials 2022-03-05 [PMID: 35247634]
Mou KJ, Shen KF, Li YL et al. Adenosine A2A Receptor in Bone Marrow-Derived Cells Mediated Macrophages M2 Polarization via PPAR gamma-P65 Pathway in Chronic Hypoperfusion Situation Frontiers in aging neuroscience 2022-01-03 [PMID: 35046793] (IHC-P, ICC/IF, Mouse)	IHC-P, ICC/IF	Mouse
Radic Shechter K, Kafkia E, Zirngibl K et al. Metabolic memory underlying minimal residual disease in breast cancer Molecular Systems Biology 2021-10-25 [PMID: 34694069]
Maisonneuve C, Tsang D, Foerster E Et Al. Nod1 Promotes Colorectal Carcinogenesis by Regulating the Immunosuppressive Functions of Tumor-Infiltrating Myeloid Cells Cell Rep 2021-01-27 [PMID: 33503439]
Chen J, Shao Y, Sasore T et al. Interphotoreceptor Retinol-Binding Protein Ameliorates Diabetes-Induced Retinal Dysfunction and Neurodegeneration Through Rhodopsin Diabetes 2021-03-01 [PMID: 33334874]
Pan X, Zhang K, Shen C et al. Astaxanthin promotes M2 macrophages and attenuates cardiac remodeling after myocardial infarction by suppression inflammation in rats Chin. Med. J. 2020-08-05 [PMID: 32701588] (FLOW, Rat) Details: Citation using the Allophycocyanin format of this antibody.	FLOW	Rat
Hozain S, Cottrell J CDllb+ targeted depletion of macrophages negatively affects bone fracture healing Bone 2020-06-12 [PMID: 32535290] (IHC-P, Mouse)	IHC-P	Mouse
Cai C, Zhao C, Kilari S et al. Effect of Sex Differences in Treatment Response to Angioplasty in a Murine Arteriovenous Fistula Model Am. J. Physiol. Renal Physiol. 2019-12-09 [PMID: 31813252]
Che Y, Shen DF, Wang ZP et al. Protective role of berberine in isoprenaline-induced cardiac fibrosis in rats BMC Cardiovasc Disord 2019-10-15 [PMID: 31615408] (FLOW, Rat)	FLOW	Rat
Gousset K, Gordon A, Kumar Kannan S, Tovar J. A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions Int J Mol Sci 2019-03-07 [PMID: 30866487] (ICC/IF, Mouse)	ICC/IF	Mouse
Choi SH, Kim AR, Nam JK, et al. Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization. Nat Commun. 2018-11-30 [PMID: 30504836] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Cao Y, Xu Y, Auchoybur ML et al. Regulatory role of IKKa in myocardial ischemia/reperfusion injury by the determination of M1 versus M2 polarization of macrophages J. Mol. Cell. Cardiol. 2018-08-25 [PMID: 30153439] (ICC/IF, Mouse)	ICC/IF	Mouse
Yang A, Herter-Sprie G, Zhang H, et al. Autophagy Sustains Pancreatic Cancer Growth through Both Cell-Autonomous and Nonautonomous Mechanisms. Cancer Discov. 2018-01-09 [PMID: 29317452] (IF/IHC, Mouse)	IF/IHC	Mouse
Yan J, Tie G, Wang S et al. Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages Nat Commun 2018-01-02 [PMID: 29295997] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Show All 21 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	Immunofluorescence - paraffin	Human	03/09/2019	View Summary Application Other Sample Tested Hepatocellular carcinoma Species Human Lot I don't have it any more Comments Comments pH9 antigen retrieval

Array NBP1-32731

• Arginase 1/ARG1/liver Arginase Antibodies
• Arginase 1/ARG1/liver Arginase ELISA Kit
• Arginase 1/ARG1/liver Arginase Kit
• Arginase 1/ARG1/liver Arginase Lysate
• Arginase 1/ARG1/liver Arginase Proteins
• Arginase 1/ARG1/liver Arginase Proteins and Enzyme