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Recombinant Mouse APRIL/TNFSF13 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

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Recombinant Mouse APRIL/TNFSF13 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 146 amino acids corresponding to APRIL/TNFSF13 Source: E. coli

Uniprot ID: Q9D777

Amino Acid Sequence: AVLTQKHKKK HSVLHLVPVN ITSKADSDVT EVMWQPVLRR GRGLEAQGDI VRVWDTGIYL LYSQVLFHDV TFTMGQVVSR EGQGRRETLF RCIRSMPSDP DRAYNSCYSA GVFHLHQGDI ITVKIPRANA KLSLSPHGTF LGFVKL

Details of Functionality
TNFSF13 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using activated T cells.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TNFSF13
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of APRIL/TNFSF13 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Mouse APRIL/TNFSF13 Protein

  • A proliferation-inducing ligand
  • APRIL
  • APRILFLJ57090
  • CD256 antigen
  • CD256
  • sAPRIL
  • sAPRIL/TNFSF13
  • TALL2
  • TALL-2
  • TNF- and APOL-related leukocyte expressed ligand 2
  • TNF-related death ligand 1
  • TNFSF13
  • TRDL1
  • TRDL-1
  • tumor necrosis factor (ligand) superfamily, member 13
  • tumor necrosis factor ligand superfamily member 13
  • tumor necrosis factor-like protein ZTNF2
  • tumor necrosis factor-related death ligand-1
  • ZTNF2

Background

A proliferation-inducing ligand (APRIL), also known as TALL-2 and TRDL-1, is a type II transmembrane protein that is a member of the TNF ligand superfamily (TNFSF13). APRIL expression is low in normal tissues, but is elevated in several types of tumors and transformed cell lines. APRIL stimulates proliferation of tumor cell lines and increases tumorigenicity in nude mice. Recombinant murine soluble APRIL is a 16.4 kDa protein, consisting of 146 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TNFSF13