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Apolipoprotein A-IV/ApoA4 Antibody (CL0465)

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Western Blot: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Lane 1: Marker [kDa], Lane 2: Human plasma
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human colon shows strong positivity in plasma.
Immunohistochemistry: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human kidney shows strong immunoreactivity in renal tubules.
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human pancreas shows absence of immunoreactivity.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CL0465
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Apolipoprotein A-IV/ApoA4 Antibody (CL0465) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LEGLTFQMKKNAEELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQKSLAELGGHLDQQVEEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVEGHLSFLEKDLRDKVNSFFSTFKEKESQDKTLSLP
Epitope
LRQRLAPLAEDVRGN
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
APOA4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Apolipoprotein A-IV/ApoA4 Recombinant Protein Antigen (NBP2-52930PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Apolipoprotein A-IV/ApoA4 Antibody (CL0465)

  • apo-AIV
  • apoA-IV
  • Apolipoprotein A4
  • Apolipoprotein AIV
  • Apolipoprotein A-IV
  • MGC142154
  • MGC142156

Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Apolipoprotein A-IV/ApoA4 Antibody (NBP2-52930). (Showing 1 - 1 of 1 FAQ).

  1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
    • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APOA4