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Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372)

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Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203] - Formalin-fixed, paraffin-embedded human liver stained with Apolipoprotein A-IV/ApoA4 Mouse Monoclonal Antibody (APOA4/3372).
Protein Array: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Apolipoprotein A-IV/ApoA4 Mouse Monoclonal Antibody ...read more
Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203] - Formalin-fixed, paraffin-embedded human liver carcinoma stained with Apolipoprotein A-IV/ApoA4 Mouse Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, MA
Clone
APOA4/3372
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08947)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Human recombinant Apolipoprotein A-IV/ApoA4 protein fragment (around aa 239-371) (exact sequence is proprietary) (Uniprot: P06727)
Localization
Secreted.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APOA4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372)

  • apo-AIV
  • apoA-IV
  • Apolipoprotein A4
  • Apolipoprotein AIV
  • Apolipoprotein A-IV
  • MGC142154
  • MGC142156

Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein A-IV/ApoA4 Antibody (NBP3-07203) (0)

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FAQs for Apolipoprotein A-IV/ApoA4 Antibody (NBP3-07203). (Showing 1 - 1 of 1 FAQ).

  1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
    • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol APOA4
Uniprot