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Annexin A2 Antibody (666316)

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 (2 Reviews)
 
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A2 Monoclonal Antibody (Catalog # MAB3928) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with ...read more
Annexin A2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human/Mouse/Rat Annexin A2 Monoclonal Antibody (Catalog # MAB3928) at 15 µg/mL overnight at 4 °C. Before incubation ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A2 at approximately 43 kDa (as indicated) using 1 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clone
666316
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3928-SP
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Annexin A2 Antibody (666316) Summary

Immunogen
E. coli-derived recombinant human Annexin A2
Met1-Asp339
Accession # P07355
Specificity
Detects human Annexin A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant human (rh) Annexin A1, A7, A9, A11, and A13 is observed. No cross-reactivity with rhAnnexin A3, A4, A6, A8, or A10 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ANXA2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 2 Reviews rated 4
using
MAB3928 in the following applications:
Publications
Read Publication using
MAB3928 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin A2 Antibody (666316)

  • Annexin A2
  • Annexin II
  • annexin-2
  • ANX2
  • ANX2L4
  • ANX2L4LPC2
  • ANXA2
  • CAL1H
  • Calpactin I heavy chain
  • calpactin I heavy polypeptide
  • Calpactin-1 heavy chain
  • chromobindin 8
  • chromobindin-8
  • LIP2
  • LIP2PAP-IV
  • Lipocortin II
  • Lipocortin-2
  • LPC2D
  • LPC2DP36
  • p36
  • PAP-IV
  • Placental anticoagulant protein IV
  • Protein I

Background

Annexin A2 (ANXA2), also known as Annexin II and Lipocortin II (LPC2), is an approximately 40 kDa member of the Annexin family of calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Phosphorylation of Annexin A2 at Tyr23 regulates its involvement in endosomal trafficking and actin cytoskeleton rearrangement. The Annexins consist of a unique amino terminal domain followed by a homologous C‑terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60-70 amino acid (aa) annexin repeats. Annexin A2 also functions as an autocrine factor to enhance osteoclast formation and bone resorption and is a major cellular substrate of the tyrosine kinase Src. Human Annexin A2 shares 97% identity with mouse and rat Annexin A2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Annexin A2 Antibody (MAB3928)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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(1)
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Reviews for Annexin A2 Antibody (MAB3928) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using MAB3928:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 IF Rat 02/20/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23332155
SpeciesRat
  4
reviewed by:
Verified Customer
 IF Human 02/20/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23711726
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Annexin A2 Antibody (MAB3928) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0
 
 Verified Customer
02/20/2015
Application: IF
Species: Rat
read this review
 
 Verified Customer
02/20/2015
Application: IF
Species: Human
read this review

Bioinformatics

Gene Symbol ANXA2
Uniprot