Angiopoietin-2 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids (aa) in length. It contains one coiled-coil domain (aa 166-248) that mediates multimerization, and a C-terminal fibrinogen-like domain (aa 275-495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang-2 is 65-66 kDa in size. Under nonreducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250-300 kDa tetramers and pentamers. Alternate splicing generates a short isoform that lacks 52 amino acids (aa) preceding the coiled-coil domain. Mature human Ang-2 shares 86% aa sequence identity with mouse and rat Ang-2. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies. Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2. While Ang-1 is a potent Tie-2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang-2 becomes as a Tie-2 agonist. The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2. Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression. Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development. It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation. Ang-2 is upregulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue. Its direct interaction with Integrins promotes tumor cell invasion. Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-P, Neutralization, Western Blot.

Showing Publications 1 - 10 of 27. Show All 27 Publications. Collapse Publications.

Publications using AF623	Applications	Species
Roos-Mattila, M;Kaprio, T;Mustonen, H;Hagström, J;Saharinen, P;Haglund, C;Seppänen, H; The possible dual role of Ang-2 in the prognosis of pancreatic cancer Scientific reports 2023-10-31 [PMID: 37907568] (IHC, Human)	IHC	Human
S Schrenk, LJ Bischoff, J Goines, Y Cai, S Vemaraju, Y Odaka, SR Good, JS Palumbo, S Szabo, D Reynaud, CD Van Raamsd, RA Lang, E Boscolo MEK inhibition reduced vascular tumor growth and coagulopathy in a mouse model with hyperactive GNAQ Nature Communications, 2023-04-06;14(1):1929. 2023-04-06 [PMID: 37024491] (Western Blot, Human)	Western Blot	Human
Simone Lasagni, Filippo Leonardi, Alessandra Pivetti, Lorenza Di Marco, Federico Ravaioli, Matteo Serenari et al. Endothelial angiopoietin-2 overexpression in explanted livers identifies subjects at higher risk of recurrence of hepatocellular carcinoma after liver transplantation Frontiers in Oncology 9/8/2022 [PMID: 36158651]
M Hultström, K Fromell, A Larsson, B Persson, B Nilsson, SE Quaggin, C Betsholtz, R Frithiof, M Lipcsey, M Jeansson Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients Biomedicines, 2022-06-06;10(6):. 2022-06-06 [PMID: 35740360] (IHC, Human)	IHC	Human
YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764. 2022-03-11 [PMID: 35360491] (Western Blot, Mouse)	Western Blot	Mouse
Ethan A. Winkler, Chang N. Kim, Jayden M. Ross, Joseph H. Garcia, Eugene Gil, Irene Oh et al. A single-cell atlas of the normal and malformed human brain vasculature Science 3/4/2022 [PMID: 35084939]
Thorben Pape, Temitayo Opemipo Idowu, Valerie Maritta Etzrodt, Klaus Stahl, Benjamin Seeliger, Hermann Haller et al. Modulation of the Permeability-Inducing Factor Angiopoietin-2 Through Bifonazole in Systemic Inflammation Shock 12/1/2021 [PMID: 33756505]
Erica Villa, Rosina Critelli, Simone Lasagni, Alessandra Melegari, Angela Curatolo, Ciro Celsa et al. Dynamic angiopoietin-2 assessment predicts survival and chronic course in hospitalized patients with COVID-19 Blood Advances 2/9/2021 [PMID: 33560382]
Boksik Cha, Yen-Chun Ho, Xin Geng, Md. Riaj Mahamud, Lijuan Chen, Yeunhee Kim et al. YAP and TAZ maintain PROX1 expression in the developing lymphatic and lymphovenous valves in response to VEGF-C signaling Development 12/1/2020 [PMID: 33060128]
Gesa L. Tiemeier, Rozemarijn de Koning, Gangqi Wang, Sarantos Kostidis, Rosalie G. J. Rietjens, Wendy M. P. J. Sol et al. Lowering the increased intracellular pH of human-induced pluripotent stem cell-derived endothelial cells induces formation of mature Weibel-Palade bodies Stem Cells Translational Medicine 7/1/2020 [PMID: 32163224]
Md. Riaj Mahamud, Xin Geng, Yen-Chun Ho, Boksik Cha, Yuenhee Kim, Jing Ma et al. GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126 Development 11/1/2019 [PMID: 31582413]
Thomas Mueller, Juana Freystein, Henrike Lucas, Hans-Joachim Schmoll Efficacy of a Bispecific Antibody Co-Targeting VEGFA and Ang-2 in Combination with Chemotherapy in a Chemoresistant Colorectal Carcinoma Xenograft Model Molecules 8/7/2019 [PMID: 31394786]
Margherita Pergolizzi, Laura Bizzozero, Elena Riccitelli, Davide Pascal, Anna Valeria Samarelli, Federico Bussolino et al. Modulation of Angiopoietin 2 release from endothelial cells and angiogenesis by the synaptic protein Neuroligin 2 Biochemical and Biophysical Research Communications 6/1/2018 [PMID: 29709479]
CY Lin, CF Cho, ST Bai, JP Liu, TT Kuo, LJ Wang, YS Lin, CC Lin, LC Lai, TP Lu, CY Hsieh, CN Chu, DC Cheng, YP Sher ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT Sci Rep, 2017-11-08;7(1):15108. 2017-11-08 [PMID: 29118335] (Neutralization, Human)	Neutralization	Human
Soundarya N. Selvam, Lara J. Casey, Mackenzie L. Bowman, Lindsey G. Hawke, Avery J. Longmore, Jeffrey Mewburn et al. Abnormal angiogenesis in blood outgrowth endothelial cells derived from von Willebrand disease patients Blood Coagulation & Fibrinolysis 5/5/2018 [PMID: 28362648]
J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis Sci Rep, 2017-03-09;7(0):44113. 2017-03-09 [PMID: 28276491] (ICC, Western Blot, Human)	ICC, Western Blot	Human
Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger M, Wu D, Min W Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Med, 2016-08-22;22(9):1033-1042. 2016-08-22 [PMID: 27548575] (Western Blot, Human)	Western Blot	Human
J Rautiola, A Lampinen, T Mirtti, A Ristimäki, H Joensuu, P Bono, P Saharinen Association of Angiopoietin-2 and Ki-67 Expression with Vascular Density and Sunitinib Response in Metastatic Renal Cell Carcinoma PLoS ONE, 2016-04-21;11(4):e0153745. 2016-04-21 [PMID: 27100185] (IHC-P, Human)	IHC-P	Human
Sampedro-Nunez M, Luque RM, Ramos-Levi AM et al. Presence of sst5TMD4, a truncated splice variant of the somatostatin receptor subtype 5, is associated to features of increased aggressiveness in pancreatic neuroendocrine tumors. Oncotarget [PMID: 26673010] Details: Used the Alexa Fluor 488 form of this antibody.
Hyun-Jung Choi, Haiying Zhang, Hongryeol Park, Kyu-Sung Choi, Heon-Woo Lee, Vijayendra Agrawal et al. Yes-associated protein regulates endothelial cell contact-mediated expression of angiopoietin-2 Nature Communications 5/12/2015 [PMID: 25962877]
Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P Endothelial destabilization by angiopoietin-2 via integrin beta1 activation. Nat Commun, 2015-01-30;6(0):5962. 2015-01-30 [PMID: 25635707] (ICC, IHC, Human)	ICC, IHC	Human
Voigtlander T, David S, Thamm K, Schlue J, Metzger J, Manns M, Lankisch T Angiopoietin-2 and biliary diseases: elevated serum, but not bile levels are associated with cholangiocarcinoma. PLoS ONE, 2014-05-13;9(5):e97046. 2014-05-13 [PMID: 24823366] (IHC, Human)	IHC	Human
Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS ONE, 2014-01-02;9(1):e84179. 2014-01-02 [PMID: 24392113] (ICC, Human)	ICC	Human
Ju , Rong, Zhuang , Zhen W, Zhang , Jiasheng, Lanahan , Anthony, Kyriakides , Themis, Sessa , William, Simons , Michael Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) and syndecan-4/syntenin pathways. J Biol Chem, 2013-11-14;289(1):510-9. 2013-11-14 [PMID: 24235146] (Western Blot, Human)	Western Blot	Human
Sung-Suk Chae, Walid S. Kamoun, Christian T. Farrar, Nathaniel D. Kirkpatrick, Elisabeth Niemeyer, Annemarie M.A. de Graaf et al. Angiopoietin-2 interferes with anti-VEGFR2- induced vessel normalization and survival benefit in mice bearing gliomas Clinical Cancer Research 7/15/2010 [PMID: 20501615]
Ohlmann A, Seitz R, Braunger B, Seitz D, Bosl MR, Tamm ER Norrin promotes vascular regrowth after oxygen-induced retinal vessel loss and suppresses retinopathy in mice. J. Neurosci., 2010-01-06;30(1):183-93. 2010-01-06 [PMID: 20053900] (Western Blot, Human)	Western Blot	Human
Helfrich I, Edler L, Sucker A, Thomas M, Christian S, Schadendorf D, Augustin HG Angiopoietin-2 levels are associated with disease progression in metastatic malignant melanoma. Clin. Cancer Res., 2009-02-15;15(4):1384-92. 2009-02-15 [PMID: 19228739] (ICC, Human)	ICC	Human
Show All 27 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	3 reviewed by: Verified Customer	WB	Mouse	01/09/2015	View Summary Application Western Blot Sample Tested See PMID 22203053 Species Mouse

Array AF623

• Angiopoietin-2 Antibodies
• Angiopoietin-2 Antibody Pair
• Angiopoietin-2 Lysate
• Angiopoietin-2 Protein
• Angiopoietin-2 ELISAs
• Angiopoietin-2 Proteins and Enzymes