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AMPK gamma 1 Antibody (7H3E9) - BSA Free

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Western Blot: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Western blot analysis using PRKAG1 mAb against HEK293 (1) and PRKAG1 (AA: 230-331)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Western blot analysis using PRKAG1 mAb against HEK293 (1) and PRKAG1 (AA: 230-331)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Immunofluorescence analysis of HepG2 cells using PRKAG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) ...read more
Immunohistochemistry: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Immunohistochemical analysis of paraffin-embedded kidney tissues using PRKAG1 mouse mAb with DAB staining.
Flow Cytometry: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Flow cytometric analysis of HepG2 cells using PRKAG1 mouse mAb (green) and negative control (red).
Western Blot: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Western blot analysis using PRKAG1 mAb against human PRKAG1 (AA: 230-331) recombinant protein. (Expected MW is 37.4 kDa)
Immunohistochemistry: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using PRKAG1 mouse mAb with DAB staining.
ELISA: AMPK gamma 1 Antibody (7H3E9) [NBP2-37285] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
7H3E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37285-0.025ml
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AMPK gamma 1 Antibody (7H3E9) - BSA Free Summary

Immunogen
Purified recombinant fragment of human AMPK gamma 1 (AA: 230-331) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAG1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
37.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for AMPK gamma 1 Antibody (7H3E9) - BSA Free

  • 5'-AMP-activated protein kinase subunit gamma-1
  • 5'-AMP-activated protein kinase, gamma-1 subunit
  • AMPK gamma 1
  • AMPK gamma-1 chain
  • AMPK gamma1
  • AMPK subunit gamma-1
  • AMPKG
  • AMPKG1
  • MGC8666
  • PRKAG1
  • protein kinase, AMP-activated, gamma 1 non-catalytic subunit

Background

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1), is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpah subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKAG1