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AMPK alpha 2 Antibody (847123) [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human AMPKa2 Monoclonal Antibody (Catalog # MAB2850) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPKa2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human AMPKa2 Monoclonal Antibody ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 71 kDa (as indicated) using 20 µg/mL of ...read more
AMPKa2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human AMPKa2 Monoclonal Antibody (Catalog # MAB2850) at 8 µg/mL for 3 hours at room temperature. Cells were stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
847123
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB2850-SP
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AMPK alpha 2 Antibody (847123) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human AMPK alpha 2
Phe340-Arg552
Accession # P54646
Specificity
Detects human AMPK alpha 2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AMPK alpha 1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAA2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using MAB2850.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMPK alpha 2 Antibody (847123) [Unconjugated]

  • 5'-AMP-activated protein kinase catalytic subunit alpha-2
  • AMPK alpha 2
  • AMPK subunit alpha-2
  • AMPK2
  • AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
  • AMPK-alpha-2 chain
  • EC 2.7.11
  • EC 2.7.11.1
  • PRKAA
  • PRKAA2
  • protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and δ subunits. The alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. Active AMPK down-regulates anabolic pathways such as fatty acid and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AMPK alpha 2 Antibody (MAB2850)(2)


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Bioinformatics

Gene Symbol PRKAA2
Uniprot