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ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free

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Western Blot: ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free [NBP2-71629] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free [NBP2-71629] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH1L1.
Immunohistochemistry: ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free [NBP2-71629] - Staining of paraffin-embedded Human liver tissue using anti-ALDH1L1 mouse monoclonal antibody.
Western Blot: ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free [NBP2-71629] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.
Immunohistochemistry: ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free [NBP2-71629] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ALDH1L1 mouse monoclonal antibody.
Immunohistochemistry: ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free [NBP2-71629] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ALDH1L1 mouse monoclonal antibody.
Immunohistochemistry: ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free [NBP2-71629] - Staining of paraffin-embedded Human Kidney tissue using anti-ALDH1L1 mouse monoclonal antibody.
Immunohistochemistry: ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free [NBP2-71629] - Staining of paraffin-embedded Human prostate tissue using anti-ALDH1L1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI3D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ALDH1L1 (NP_036322) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALDH1L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
98.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ALDH1L1 Antibody (OTI3D2) - Azide and BSA Free

  • 10-formyltetrahydrofolate dehydrogenase
  • aldehyde dehydrogenase 1 family, member L1
  • aldehyde dehydrogenase family 1 member L1
  • Cytosolic 10-formyltetrahydrofolate dehydrogenase
  • DKFZp781N0997
  • EC 1.5.1.6,10-FTHFDH
  • FDH
  • formyltetrahydrofolate dehydrogenase
  • FTHFD10-fTHF

Background

ALDH1L1 is encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ALDH1L1