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Human alcohol dehydrogenase ELISA Kit (Chemiluminescence)

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ELISA: Human alcohol dehydrogenase ELISA Kit (Chemiluminescence) [NBP2-66645] - Samples were spiked with high concentrations of Human alcohol dehydrogenase and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human alcohol dehydrogenase ELISA Kit (Chemiluminescence) [NBP2-66645] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

Human alcohol dehydrogenase ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human ADH1 in samples. No significant cross-reactivity or interference between Human ADH1 and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 10.17
Intra-Assay
CV% < 9.89
Spike Recovery
86 - 112%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
ADH1A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human alcohol dehydrogenase ELISA Kit (Chemiluminescence)

  • ADH, alpha subunit
  • ADH1alcohol dehydrogenase 1A
  • alcohol dehydrogenase 1 (class I), alpha polypeptide
  • alcohol dehydrogenase 1A (class I), alpha polypeptide
  • Alcohol dehydrogenase subunit alpha
  • aldehyde reductase
  • EC 1.1.1
  • EC 1.1.1.1

Background

ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ADH1A