![Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Azide/BSA FREE version of NB600-912. Detection of yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-Antibody-Western-Blot-NB600-912-img0006.jpg "Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Azide/BSA FREE version of NB600-912. Detection of yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature.")
| Reactivity | YeSpecies Glossary |
| Applications | WB, ELISA, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Description | This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast] Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | alcohol dehydrogenase [Yeast] (Uniprot: P00330) |
| Localization | Cytoplasmic |
| Specificity | Recognises Alcohol Dehydrogenase. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ADH1A |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested by western blot and is suitable for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. |
| Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Lane 1: Alcohol Dehydrogenase. Load: 50ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37kDa, 45kDa.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-Antibody-Western-Blot-NB600-912-img0004.jpg "Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Lane 1: Alcohol Dehydrogenase. Load: 50ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37kDa, 45kDa.")
![Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Biotin conjugate of NB600-912. Lane 1: Alcohol Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alcohol Dehydrogenase (Yeast) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Streptavidin Antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37 kDa, 45 kDa for Alcohol Dehydrogenase (yeast).](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-Antibody-Western-Blot-NB600-912-img0005.jpg "Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Biotin conjugate of NB600-912. Lane 1: Alcohol Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alcohol Dehydrogenase (Yeast) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Streptavidin Antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37 kDa, 45 kDa for Alcohol Dehydrogenase (yeast).")
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