![Western Blot: AKR1A1 Antibody [NB100-1074] - Analysis of AKR1A1 in Human Placenta lysate using NB100-1074 at 1 ug/ ml.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Western-Blot-NB100-1074-img0005.jpg "Western Blot: AKR1A1 Antibody [NB100-1074] - Analysis of AKR1A1 in Human Placenta lysate using NB100-1074 at 1 ug/ ml.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-DAGHPLYPFNDPY corresponding to C-Terminus according to NP_697021.1. |
| Localization | Secreted |
| Specificity | Both reported variants (NP_006057.1and NP_697021.1) represent identical protein |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | AKR1A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 37 kDa band observed in human liver and human placenta lysates. In transfected HEK293 transiently expressing human AKR1A1 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293. (calculated MW of 36.6 kDa band according to NP_006057.1 and NP_697021.1). IHC-P: Human kidney shows Brush Border staining in PCT. |
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| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Human Liver Whole Tissue Lysate (Adult Whole Normal) | |
| AKR1A1 Overexpression Lysate | |
| Human Placenta Whole Tissue Lysate (Adult Whole Normal) |
Secondary Antibodies |
Isotype Controls |
Research Areas for AKR1A1 Antibody (NB100-1074)Find related products by research area.
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![Immunohistochemistry-Paraffin: AKR1A1 Antibody [NB100-1074] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunohistochemistry-Paraffin-NB100-1074-img0007.jpg "Immunohistochemistry-Paraffin: AKR1A1 Antibody [NB100-1074] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.")
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![Immunohistochemistry-Paraffin: AKR1A1 Antibody [NB100-1074] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunohistochemistry-Paraffin-NB100-1074-img0006.jpg "Immunohistochemistry-Paraffin: AKR1A1 Antibody [NB100-1074] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.")
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