![Immunocytochemistry/Immunofluorescence: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml, showing cytoplasmic staining. The nuclear stain is DAPI (blue)](http://images.novusbio.com/fullsize/Adenosine-A2b-R-Antibody-Immunocytochemistry-Immunofluorescence-NB600-797-img0005.jpg "Immunocytochemistry/Immunofluorescence: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml, showing cytoplasmic staining. The nuclear stain is DAPI (blue)")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence CQADVKSGNGQ corresponding to C-Terminus according to NP_000667.1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ADORA2B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC: In paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Flow Cytometry: Flow cytometric analysis of Kelly cells. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
| Publication using NB600-797 | Applications | Species |
|---|---|---|
| Simard T, Jung R, Di Santo P et al. Dipyridamole and vascular healing following stent implantation Frontiers in cardiovascular medicine 2023-09-08 [PMID: 37745122] (IHC, KD, WB, Rabbit) | IHC, KD, WB | Rabbit |
Secondary Antibodies |
Isotype Controls |
Research Areas for Adenosine A2b R Antibody (NB600-797)Find related products by research area.
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![Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NB600-797] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Adenosine-A2b-R-Antibody-Immunohistochemistry-Paraffin-NB600-797-img0002.jpg "Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NB600-797] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Flow Cytometry: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line)](http://images.novusbio.com/fullsize/Adenosine-A2b-R-Antibody-Flow-Cytometry-NB600-797-img0003.jpg "Flow Cytometry: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line)")
![Immunocytochemistry/Immunofluorescence: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml, showing cytoplasmic staining. The nuclear stain is DAPI (blue)](http://images.novusbio.com/fullsize/Adenosine-A2b-R-Antibody-Immunocytochemistry-Immunofluorescence-NB600-797-img0004.jpg "Immunocytochemistry/Immunofluorescence: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml, showing cytoplasmic staining. The nuclear stain is DAPI (blue)")
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