Novus Biologicals products are now on bio-techne.com

ACE-2 Antibody (535919) [Alexa Fluor® 647]

Images

 
HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse anti-Human Alexa Fluor® 647-conjugated ACE-2 Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
535919
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Order Details

ACE-2 Antibody (535919) [Alexa Fluor® 647] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740
Accession # Q9BYF1
Specificity
Detects human ACE-2 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Publications
Read Publications using
FAB9332R in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Reconstitution Instructions
Reconstitution default not known

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody (535919) [Alexa Fluor® 647]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

Angiogensin I Converting Enzyme-2 (ACE-2), also called ACEH (ACE homolog), is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function (1). ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-62346
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
AF1513
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
DAN00
Species: Hu
Applications: ELISA
NBP1-78444
Species: Hu, Mu, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
MAB4447
Species: Hu
Applications: IHC, WB
NBP1-77078
Species: Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF1126
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
H00009197-M07
Species: Ha, Hu, Mu, Rt
Applications: ELISA, ICC/IF, KD, WB
NB100-309
Species: Hu, Pm, Mu, Rt
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD, PAGE, Single-Cell Western, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
DCP00
Species: Hu
Applications: ELISA
AF4965
Species: Mu
Applications: IHC, Simple Western, WB
NB100-1519
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-FrFl, PEP-ELISA, WB
M6000B
Species: Mu
Applications: ELISA

Publications for ACE-2 Antibody (FAB9332R)(7)

We have publications tested in 5 confirmed species: Human, Chinese Hamster, Hamster, Mesocricetus auratus (Hamster), Primate - C. aethiops.

We have publications tested in 2 applications: Flow Cyomtetry, Flow Cytometry.


Filter By Application
Flow Cyomtetry
(1)
Flow Cytometry
(7)
All Applications
Filter By Species
Human
(7)
Chinese Hamster
(1)
Hamster
(1)
Mesocricetus auratus (Hamster)
(1)
Primate - C. aethiops
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using FAB9332R Applications Species
AD López-Muño, I Kosik, J Holly, JW Yewdell Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunity Science Advances, 2022-08-03;8(31):eabp9770. 2022-08-03 [PMID: 35921414] (Flow Cytometry, Human) Flow Cytometry Human
L El-Shennaw, AD Hoffmann, NK Dashzeveg, KM McAndrews, PJ Mehl, D Cornish, Z Yu, VL Tokars, V Nicolaescu, A Tomatsidou, C Mao, CJ Felicelli, CF Tsai, C Ostiguin, Y Jia, L Li, K Furlong, J Wysocki, X Luo, CF Ruivo, D Batlle, TJ Hope, Y Shen, YK Chae, H Zhang, VS LeBleu, T Shi, S Swaminatha, Y Luo, D Missiakas, GC Randall, AR Demonbreun, MG Ison, R Kalluri, D Fang, H Liu Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2 Nature Communications, 2022-01-20;13(1):405. 2022-01-20 [PMID: 35058437] (Flow Cytometry, Human) Flow Cytometry Human
GM Castiglion, L Zhou, Z Xu, Z Neiman, CF Hung, EJ Duh Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by�epistasis acting on ACE2 PloS Biology, 2021-12-21;19(12):e3001510. 2021-12-21 [PMID: 34932561] (Flow Cytometry, Human) Flow Cytometry Human
AD López-Muño, I Kosik, J Holly, JW Yewdell Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity bioRxiv : the preprint server for biology, 2021-12-13;0(0):. 2021-12-13 [PMID: 34931195] (Flow Cyomtetry, Flow Cytometry, Hamster, Human, Primate - C. aethiops) Flow Cyomtetry, Flow Cytometry Hamster, Human, Primate - C. aethiops
J Yewdell, A Lopez-Muno, I Kosik, J Holly Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity Research square, 2021-12-13;0(0):. 2021-12-13 [PMID: 34931184] (Flow Cytometry, Chinese Hamster, Human, Mesocricetus auratus (Hamster)) Flow Cytometry Chinese Hamster, Human, Mesocricetus auratus (Hamster)
A Hudák, A Letoha, L Szilák, T Letoha Contribution of Syndecans to the Cellular Entry of SARS-CoV-2 International Journal of Molecular Sciences, 2021-05-19;22(10):. 2021-05-19 [PMID: 34069441] (Flow Cytometry, Human) Flow Cytometry Human
R Liu, JL Americo, CA Cotter, PL Earl, N Erez, C Peng, B Moss MVA Vector Vaccines Inhibit SARS CoV-2 Replication in Upper and Lower Respiratory Tracts of Transgenic Mice and Prevent Lethal Disease bioRxiv : the preprint server for biology, 2021-01-01;0(0):. 2021-01-01 [PMID: 33442693] (Flow Cytometry, Human) Flow Cytometry Human

Reviews for ACE-2 Antibody (FAB9332R) (0)

There are no reviews for ACE-2 Antibody (FAB9332R). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ACE-2 Antibody (FAB9332R) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ACE-2 Products

Blogs on ACE-2.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.

COVID-19 and the Cardiovascular System: Observed complications and potential mechanisms
By Victoria OsinskiThe outbreak of COVID-19 resulting from the transmission of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in many cases of illness typically manifesting in mi...  Read full blog post.


  Read full blog post.


  Read full blog post.

Tiny Antibodies (VHHs) from Llama Neutralize Respiratory Coronaviruses
By Jamshed Arslan, Pharm. D., PhD. VHH Single Domain Antibodies vs Conventional AntibodiesThe immune system protects living organisms against harmful substances. B cells ward off infections by producing antibodies t...  Read full blog post.

Blocking SARS-CoV-2 Cell Entry: A potential Strategy Against COVID-19 Pandemic
By Jamshed Arslan, Pharm. D., PhD. Coronaviruses are a family of enveloped RNA viruses. Some family members circulate in human populations, but others like severe acute respiratory syndrome coronavirus (SARS-CoV) ar...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ACE-2 Antibody (535919) [Alexa Fluor® 647] and receive a gift card or discount.

Bioinformatics

Uniprot