ABCG2/CD338 Antibody (5D3) [Allophycocyanin]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34^- cells and is downregulated with the acquisition of CD34 on the cell surface (6).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, ICC.

Showing Publications 1 - 6 of 6.

Publications using FAB995A	Applications	Species
G Gallerani, T Rossi, M Valgiusti, D Angeli, P Fici, S De Fanti, E Bandini, C Cocchi, GL Frassineti, M Bonafè, F Fabbri CNA Profiling of Single CTCs in Locally Advanced Esophageal Cancer Patients during Therapy Highlights Unexplored Molecular Pathways Cancers, 2021-12-19;13(24):. 2021-12-19 [PMID: 34944989] (ICC, Human)	ICC	Human
Zhao Y, Bao Q, Schwarz B, Zhao L, Mysliwietz J, Ellwart J, Renner A, Hirner H, Niess H, Camaj P, Angele M, Gros S, Izbicki J, Jauch K, Nelson P, Bruns C Stem cell-like side populations in esophageal cancer: a source of chemotherapy resistance and metastases. Stem Cells Dev, 2013-11-13;23(2):180-92. 2013-11-13 [PMID: 24021093] (Flow Cytometry, Human)	Flow Cytometry	Human
Gojo I, Tan M, Fang H, Sadowska M, Lapidus R, Baer M, Carrier F, Beumer J, Anyang B, Srivastava R, Espinoza-Delgado I, Ross D Translational phase I trial of vorinostat (suberoylanilide hydroxamic acid) combined with cytarabine and etoposide in patients with relapsed, refractory, or high-risk acute myeloid leukemia. Clin Cancer Res, 2013-02-12;19(7):1838-51. 2013-02-12 [PMID: 23403629] (Flow Cytometry, Human)	Flow Cytometry	Human
Ozvegy-Laczka C, Laczko R, Hegedus C, Litman T, Varady G, Goda K, Hegedus T, Dokholyan NV, Sorrentino BP, Varadi A, Sarkadi B Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter. J. Biol. Chem., 2008-07-21;283(38):26059-70. 2008-07-21 [PMID: 18644784] (Flow Cytometry, Human)	Flow Cytometry	Human
Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G MDR1 expression identifies human melanoma stem cells. Biochem. Biophys. Res. Commun., 2008-02-13;368(4):930-6. 2008-02-13 [PMID: 18279661] (Flow Cytometry, Human)	Flow Cytometry	Human
Bram EE, Ifergan I, Grimberg M, Lemke K, Skladanowski A, Assaraf YG C421 allele-specific ABCG2 gene amplification confers resistance to the antitumor triazoloacridone C-1305 in human lung cancer cells. Biochem. Pharmacol., 2007-04-01;74(1):41-53. 2007-04-01 [PMID: 17481587] (Flow Cytometry, Human)	Flow Cytometry	Human

Array FAB995A

• ABCG2/CD338 Antibodies
• ABCG2/CD338 ELISA Kits
• ABCG2/CD338 Protein