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ABCG2/CD338 Antibody (3G8) - Azide and BSA Free

Images

 
Western Blot: ABCG2/CD338 Antibody (3G8) - Azide and BSA Free [NBP2-80558] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts. Image from the standard ...read more
Immunocytochemistry: ABCG2/CD338 Antibody (3G8) - Azide and BSA Free [NBP2-80558] - Analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB ...read more
Flow Cytometry: ABCG2/CD338 Antibody (3G8) - Azide and BSA Free [NBP2-80558] - Analysis of HepG2 cells using ABCG2 mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: ABCG2/CD338 Antibody (3G8) - Azide and BSA Free [NBP2-80558] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been ...read more
ELISA: ABCG2/CD338 Antibody (3G8) - Azide and BSA Free [NBP2-80558] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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ABCG2/CD338 Antibody (3G8) - Azide and BSA Free Summary

Immunogen
Purified recombinant fragment of human ABCG2 expressed in E. coli. [Uniprot: Q9UNQ0]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ABCG2
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
In Western blot, a dimer can be seen at 60-70 kDa representing ABCG2.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for ABCG2/CD338 Antibody (3G8) - Azide and BSA Free

  • ABC transporter
  • ABC15
  • ABCG2
  • ABCP
  • ABCPMGC102821
  • ATP-binding cassette sub-family G member 2
  • ATP-binding cassette transporter G2
  • ATP-binding cassette, sub-family G (WHITE), member 2
  • BCRP
  • BCRP1
  • BCRPMRX
  • BMDP
  • Breast cancer resistance protein
  • CD338 antigen
  • CD338
  • CDw338
  • EST157481
  • mitoxantrone resistance protein
  • Mitoxantrone resistance-associated protein
  • MXR
  • MXRMXR1
  • placenta specific MDR protein
  • Placenta-specific ATP-binding cassette transporter

Background

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ABCG2