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5T4 Antibody [Unconjugated]

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Retinoic acid-treated D3 mouse embryonic stem cell line was stained with Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).
Retinoic acid-treated D3 mouse embryonic stem cell line was stained with Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049, filled histogram) or isotype control antibody (Catalog # ...read more
5T4 was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF5049
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5049-SP
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5T4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse 5T4
Ser30-Ser361
Accession # AAH58198
Specificity
Detects mouse 5T4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human 5T4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TPBG
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF5049 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5T4 Antibody [Unconjugated]

  • 5T4 oncofetal trophoblast glycoprotein
  • 5T4 oncotrophoblast glycoprotein
  • 5T4
  • 5T4AG
  • 5T4-AG
  • 5T4M6P1,5T4 oncofetal antigen
  • M6P1
  • TPBG
  • trophoblast glycoprotein

Background

5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72‑80 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and differentiating embryonic stem cells and impacts cell adhesion and motility. The mouse 5T4 cDNA encodes a type I transmembrane protein precursor that is 426 amino acids (aa) in length. It contains a 332 aa extracellular region (aa 30‑361) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90‑338). Over aa 30‑361, mouse 5T4 shares 95% and 81% aa identity with rat and human 5T4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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5T4 Antibody
[Unconjugated]
AF5049
5T4 Antibody [Unconjugated]
Species: Mu
Applications: WB, Flow, CyTOF-ready, ICC/IF

Publications for 5T4 Antibody (AF5049)(6)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 4 applications: Flow Cytometry, IHC, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC
(3)
Immunoprecipitation
(1)
Western Blot
(2)
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Filter By Species
Mouse
(4)
Transgenic Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF5049 Applications Species
Lyons-Warren, AM;Tantry, EK;Moss, EH;Kochukov, MY;Belfort, BDW;Ortiz-Guzman, J;Freyberg, Z;Arenkiel, BR; Co-transmitting interneurons in the mouse olfactory bulb regulate olfactory detection and discrimination Cell reports 2023-11-18 [PMID: 37980561] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Colin M. Wakeham, Gaoying Ren, Catherine W. Morgans Expression and distribution of trophoblast glycoprotein in the mouse retina Journal of Comparative Neurology 7/1/2020 [PMID: 31891182]
K Matsuoka, Y Kohara, Y Naoe, A Watanabe, M Ito, K Ikeda, S Takeshita WAIF1 is a cell-surface CTHRC1 binding protein coupling bone resorption and formation J. Bone Miner. Res., 2018-05-11;0(0):. 2018-05-11 [PMID: 29624737] (Flow Cytometry, Immunoprecipitation, Western Blot, Mouse) Flow Cytometry, Immunoprecipitation, Western Blot Mouse
Y Yu, AH Moberly, JP Bhattarai, C Duan, Q Zheng, F Li, H Huang, W Olson, W Luo, T Wen, H Yu, M Ma The Stem Cell Marker Lgr5 Defines a Subset of Post-Mitotic Neurons in the Olfactory Bulb J. Neurosci., 2017-08-28;0(0):. 2017-08-28 [PMID: 28847812] (IHC, Mouse) IHC Mouse
A Subtype of Olfactory Bulb Interneurons Is Required for Odor Detection and Discrimination Behaviors J Neurosci, 2016-08-03;36(31):8210-27. 2016-08-03 [PMID: 27488640] (IHC, Mouse) IHC Mouse
Rosu-Myles M, She YM, Fair J Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells. PLoS ONE, 2012-06-13;7(6):e38954. 2012-06-13 [PMID: 22719999] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol TPBG
Uniprot