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5T4 Antibody [Unconjugated]

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Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4975) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for 5T4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4975) followed by ...read more
5T4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Sheep Anti-Human 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4975) overnight at 4 °C. Before ...read more
5T4 was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4975) at 10 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of human placenta, loaded at 0.2 mg/mL. A specific band was detected for 5T4 at approximately 90 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human 5T4 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4975
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4975-SP
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5T4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human 5T4
Ser31-Ser355
Accession # Q13641
Specificity
Detects human 5T4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse 5T4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TPBG
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4975 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5T4 Antibody [Unconjugated]

  • 5T4 oncofetal trophoblast glycoprotein
  • 5T4 oncotrophoblast glycoprotein
  • 5T4
  • 5T4AG
  • 5T4-AG
  • 5T4M6P1,5T4 oncofetal antigen
  • M6P1
  • TPBG
  • trophoblast glycoprotein

Background

Human 5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium, and embryonic stem cells and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420 amino acids (aa) in length. It contains a 324 aa extracellular region (aa 32-355) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90-355). Over aa 31-355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5T4 Antibody (AF4975)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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Bioinformatics

Gene Symbol TPBG
Uniprot