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5T4 Antibody (524744) [Phycoerythrin]

Images

 
BG01V human embryonic stem cells were stained with Mouse Anti-Human 5T4 PE-conjugated Monoclonal Antibody (Catalog # FAB49751P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our ...read more
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human 5T4 PE-conjugated Monoclonal Antibody (Catalog # FAB49751P, filled histogram) or isotype control antibody (IC002P, open histogram). Staining was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
524744
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

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5T4 Antibody (524744) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human 5T4
Ser31-Ser355
Accession # Q13641
Specificity
Detects human 5T4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5T4 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TPBG
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5T4 Antibody (524744) [Phycoerythrin]

  • 5T4 oncofetal trophoblast glycoprotein
  • 5T4 oncotrophoblast glycoprotein
  • 5T4
  • 5T4AG
  • 5T4-AG
  • 5T4M6P1,5T4 oncofetal antigen
  • M6P1
  • TPBG
  • trophoblast glycoprotein

Background

Human 5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and embryonic stem cells, and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420 amino acids (aa) in length. It contains a 324 aa extracellular region (aa 32‑355) that shows one Ser‑rich region followed by seven Leu-rich repeats (aa 90‑355). Over aa 31‑355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TPBG
Uniprot