Recombinant Human 4-1BB Ligand/TNFSF9 Protein Summary
Description |
A single non-glycosylated polypeptide chain containing 184 amino acids corresponding to 4-1BB Ligand/TNFSF9 Source: E. coli Uniprot ID: P41273 Amino Acid Sequence: REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions |
Details of Functionality |
Fully biologically active when compared to standard. The ED50 as determined by stimulation of IL-8 production using human PBMC is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
TNFSF9 |
Purity |
>95%, by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 1 EU/ug of 4-1BB Ligand/TNFSF9 as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
19.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
>95%, by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Alternate Names for Recombinant Human 4-1BB Ligand/TNFSF9 Protein
Background
41BBL is a member of the tumor necrosis factor (TNF) receptor family. This receptor contributes to the clonal expansion, survival, and development of T cells. In addition, 41BBL expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. CD137 has been shown to interact with TRAF2. The human 41BBL gene codes for a 254 amino acid type II transmembrane containing a 28 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 205 amino acid extracellular domain (ECD). The human 41BBL ECD shares 32 % and 35 % a.a. identity with murine and rat ECD.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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