Rad51 Antibodies
We offer Rad51 Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each Rad51 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Rad51 Antibodies can be used in a variety of model species: C. elegans, Chicken, Human, Mouse, Rat, Yeast. Use the list below to choose the Rad51 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Rad51 polyclonal antibodies and browse our Rad51 monoclonal antibody catalog.
Alternate Names for Rad51 Antibodies
anti-Rad51 antibody, anti-BRCC5 antibody, anti-hRAD51 antibody, anti-HsRAD51 antibody, anti-HsT16930 antibody, anti-RAD51 (S. cerevisiae) homolog (E coli RecA homolog) antibody, anti-RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody, anti-RAD51 homolog A antibody, anti-RAD51ABRCA1/BRCA2-containing complex, subunit 5 antibody, anti-RecA, E. coli, homolog of antibody, anti-RecA-like protein antibody, anti-RECADNA repair protein RAD51 homolog 1 antibody, anti-recombination protein A antibody
12 Results for "rad51" in Primary Antibodies
![Western Blot: Rad51 Antibody (14B4) [NB100-148] - eIF3f facilitates hMSH4 stabilization. Western blotting analysis of the levels of HDAC3, hRad51, and VBP1 expression in 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells. 293T/eIF3f-hMSH4 cells treated with 1 or 10 Gy IR were fractionated at 6 hrs post-treatment and the levels of hMSH4 and eIF3f in the nuclear and cytoplasmic fractions were determined by immunoblotting. Alpha-tubulin was used as a marker for the cytoplasmic fraction. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-51) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rad51-Antibody-14B4-Western-Blot-NB100-148-img0020.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad51 Antibody (14B4) [NB100-148] - eIF3f facilitates hMSH4 stabilization. Western blotting analysis of the levels of HDAC3, hRad51, and VBP1 expression in 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells. 293T/eIF3f-hMSH4 cells treated with 1 or 10 Gy IR were fractionated at 6 hrs post-treatment and the levels of hMSH4 and eIF3f in the nuclear and cytoplasmic fractions were determined by immunoblotting. Alpha-tubulin was used as a marker for the cytoplasmic fraction. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-51) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - HCC1937 (human immortalized breast cancer) cells treated with PARPi for 96 h. Nuclei are stained in blue (DAPI) and RAD51 in red. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Rad51-Antibody-14B4-Immunocytochemistry-Immunofluorescence-NB100-148-img0021.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution at 1:200. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.](http://images.novusbio.com/fullsize/Rad51-Antibody-14B4-Immunohistochemistry-Paraffin-NB100-148-img0003.jpg)
![Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Rad51-Antibody-14B4-Western-Blot-NB100-148-img0009.jpg)
![Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Rad51-Antibody-14B4-Western-Blot-NB100-148-img0011.jpg)
![Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Rad51-Antibody-14B4-Western-Blot-NB100-148-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.](http://images.novusbio.com/fullsize/Rad51-Antibody-14B4-Immunocytochemistry-Immunofluorescence-NB100-148-img0004.jpg)
![Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148] - HeLa whole cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody was used for subsequent WB detection of immunoprecipated RAD51.](http://images.novusbio.com/fullsize/Rad51-Antibody-14B4-Immunoprecipitation-NB100-148-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Rad51 Antibody (14B4) [NB100-148] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4].](http://images.novusbio.com/fullsize/Rad51-Antibody-14B4-Western-Blot-NB100-148-img0006.jpg)
![Western Blot: Rad51 Antibody (14B4) [NB100-148] - YFP-PALB2 & YFP-PALB2 146AAAA binds RAD51 equally.Lysates from HEK293T cells expressing the indicated constructs were subjected to GFP-Trap pulldown & immunoblotting against YFP or RAD51. YFP alone was used as a negative control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31017574), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-148_mouse-monoclonal-rad51-antibody-14b4-31020241534372.jpg)
![Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Bractoppin Selectively Interrupts BRCA1-Dependent Steps in DNA Repair by Homologous Recombination(A) Confocal images depicting at high magnification the recruitment of the RPA32 protein into nuclear foci after the indicated treatments. Experiments were carried out as described as in Figure 5A. Staining in the upper row is for RPA32 (green), middle row, for mCherry-BRCA1 tBRCT (red); lower row, merged red & green staining, with DNA staining (DAPI) in blue. Scale bar represents 10 μm.(B) Recruitment of RAD51 protein into nuclear foci, measured & depicted as described in (A). Scale bar represents 10 μm.(C) Percentage of cells positive for radiation-induced nuclear RPA32 foci (mean ± SD; n = 5,300, 0 Gy; 3,200, 16 Gy; 3,600, BRCA1 tBRCT; 3,400, Bractoppin; 3,500, CCBT2047) enumerated by high-content imaging (see the STAR Methods). Statistical significance was performed using an unpaired two-tailed t test. ***p ≤ 0.001. Similar results were observed in three independent repeats.(D) Percentage of cells containing nuclear RAD51 foci enumerated & depicted as described in (B). ***p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29606576), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-148_mouse-monoclonal-rad51-antibody-14b4-310202415525270.jpg)
![Western Blot: Rad51 Antibody (14B4) [NB100-148] - MiCas9’s beneficial effects are Brex27 & RAD51 dependent.a Indel rates by spCas9, miCas9, & miCas9-mutant at the on-target site for sg-AAVS1 & the off-target site 1 for sg-EMX1. b Indel rates estimated by T7E1 assay by spCas9 & miCas9 with or without the use of RAD51 inhibitor, B02. c Co-IP results by using anti-flag (left) or anti-RAD51 (right) antibodies followed western blot for RAD51 or Cas9. d ChIP assay results with anti-RAD51 antibody at the proximal region of the sg-AAVS1 target locus. Sp: spCas9, Mi: miCas9, Mi-mut: miCas9-mutant. #Reads: Average amplicon reads per sample. Three independent experiments were performed for each condition. Data are presented as mean ± standard error of means (SEM). Unpaired t-test (two tailed) was used to compare data using GraphPad Prism 8 software (GraphPad Software, Inc., San Diego, CA). Source data are available in the Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33247137), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-148_mouse-monoclonal-rad51-antibody-14b4-31020241554538.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in human colon.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0015.jpg "Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in human colon.")
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody [H00005888-B01P] - Immunofluorescence of purified MaxPab antibody to RAD51 on HeLa cell. [antibody concentration 10 ug/ml]](http://images.novusbio.com/fullsize/Rad51-Antibody-Immunocytochemistry-Immunofluorescence-H00005888-B01P-img0008.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody [H00005888-B01P] - Analysis of purified antibody to RAD51 on formalin-fixed paraffin-embedded human testis. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Rad51-Antibody-Immunohistochemistry-Paraffin-H00005888-B01P-img0010.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - expression in transfected 293T cell line. Lane 1: RAD51 transfected lysate(37.29 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0009.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in PC-12.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0011.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0012.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in Jurkat.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0013.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0014.jpg)
![Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in transfected 293T cell line by RAD51 polyclonal antibody. Lane 1: RAD51 transfected lysate(37.29 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-H00005888-B01P-img0016.jpg)
![Western Blot: Rad51 Antibody (13E4) [NB100-499] - Detection of Rad51 in HeLa whole cell extract.](http://images.novusbio.com/fullsize/Rad51-Antibody-13E4-Western-Blot-NB100-499-img0005.jpg "Western Blot: Rad51 Antibody (13E4) [NB100-499] - Detection of Rad51 in HeLa whole cell extract.")
![Western Blot: Rad51 Antibody (13E4) [NB100-499] - Sample (50ug of whole cell lysate) A: mouse testis 10% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/Rad51-Antibody-13E4-Western-Blot-NB100-499-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0007.jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Jurkat cells with Rad51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Flow-Cytometry-NBP2-75640-img0001.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded human placenta tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded mouse testis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0006.jpg)
![Western Blot: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Rad51 on different lysates with Rabbit anti-Rad51 antibody at 1/1,000 dilution.Lane 1: Jurkat cell lysate (10 µg/Lane)Lane 2: NIH/3T3 cell lysate (10 µg/Lane)Lane 3: Mouse testis tissue lysate (20 µg/Lane)Predicted band size: 37 kDaObserved band size: 37 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-western-blot-2862024111350..jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of U-2 OS cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/100 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (ET1705-96) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunocytochemistry-immunofluorescence-2862024111534..jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of NIH/3T3 cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunocytochemistry-immunofluorescence-2862024111720..jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Mouse spleen tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-2862024111850..jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Human tonsil tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-286202411203..jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Mouse testis tissue with Rabbit anti-Rad51 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-2862024112111..jpg)
![Western Blot: Rad51 Antibody [NBP2-20058] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-NBP2-20058-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rad51 Antibody [NBP2-20058] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-20058] - Human ovarian carcinoma. Rad51 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Rad51-Antibody-Immunohistochemistry-Paraffin-NBP2-20058-img0005.jpg)
![Western Blot: Rad51 Antibody [NBP2-20058] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-NBP2-20058-img0001.jpg)
![Western Blot: Rad51 Antibody [NBP2-20058] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-NBP2-20058-img0006.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-20058] - Human breast carcinoma. Rad51 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Rad51-Antibody-Immunohistochemistry-Paraffin-NBP2-20058-img0004.jpg)
![Immunoprecipitation: Rad51 Antibody [NBP2-20058] - Jurkat whole cell extract A. 40 ug Jurkat whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of Rad51 protein by 4 ug Rad51 antibody 5 % SDS-PAGE The immunoprecipitated Rad51 protein was detected by Rad51 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent].](http://images.novusbio.com/fullsize/Rad51-Antibody-Immunoprecipitation-NBP2-20058-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (523713) [Unconjugated] [MAB99481] - Rad51 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse Rad51 Monoclonal Antibody (Catalog # MAB99481) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei.](http://images.novusbio.com/fullsize/Rad51-Antibody-523713-Unconjugated-Immunocytochemistry-Immunofluorescence-MAB99481-img0001.jpg "Immunocytochemistry/Immunofluorescence: Rad51 Antibody (523713) [Unconjugated] [MAB99481] - Rad51 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse Rad51 Monoclonal Antibody (Catalog # MAB99481) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei.")
![Western Blot: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Western Blot Analysis of HeLa, HepG2, K562, A431 and MCF-7 cell lysate using Rad51 Antibody (RAD51/2701).](http://images.novusbio.com/fullsize/Rad51-Antibody-RAD51-2701-Western-Blot-NBP2-79760-img0003.jpg "Western Blot: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Western Blot Analysis of HeLa, HepG2, K562, A431 and MCF-7 cell lysate using Rad51 Antibody (RAD51/2701).")
![Flow Cytometry: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Flow Cytometric Analysis of K562 cells using Rad51 Antibody (RAD51/2701)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Rad51-Antibody-RAD51-2701-Flow-Cytometry-NBP2-79760-img0001.jpg)
![Protein Array: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Rad51 Antibody (RAD51/2701) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Rad51-Antibody-RAD51-2701-Protein-Array-NBP2-79760-img0002.jpg)
![Western Blot: Rad51 Antibody (S04-3C6) [NBP3-14968] - Western blot detection of Rad51 in A549, HL-60, U2OS cell lysates using Rad51 Rabbit mAb (1:1000 diluted). Predicted band size: 37kDa. Observed band size: 37kDa.](http://images.novusbio.com/fullsize/Rad51-Antibody-S04-3C6-Western-Blot-NBP3-14968-img0001.jpg "Western Blot: Rad51 Antibody (S04-3C6) [NBP3-14968] - Western blot detection of Rad51 in A549, HL-60, U2OS cell lysates using Rad51 Rabbit mAb (1:1000 diluted). Predicted band size: 37kDa. Observed band size: 37kDa.")
![Immunohistochemistry-Paraffin: Rad51 Antibody (S04-3C6) [NBP3-14968] - Immunohistochemistry of Rad51 in paraffin-embedded Human tonsil using Rad51 Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/Rad51-Antibody-S04-3C6-Immunohistochemistry-Paraffin-NBP3-14968-img0002.jpg)
![Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149] - Western blot analysis of extracts of various cell lines, using Rad51 Rabbit mAb (NBP3-16149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Rad51-Antibody-5S9B0-Western-Blot-NBP3-16149-img0001.jpg "Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149] - Western blot analysis of extracts of various cell lines, using Rad51 Rabbit mAb (NBP3-16149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Rad51 Antibody [NBP2-20059] - Sample (10 ug of whole cell lysate) A: yeast 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-NBP2-20059-img0001.jpg "Western Blot: Rad51 Antibody [NBP2-20059] - Sample (10 ug of whole cell lysate) A: yeast 7. 5% SDS PAGE gel, diluted at 1:1000.")