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![Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of extracts from normal (control) and PRKAR2A knockout (KO) HeLa cells, using Protein Kinase A Regulatory Subunit II alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Protein-Kinase-A-Regulatory-Subunit-II-alpha-Antibody-Western-Blot-NBP3-03477-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of extracts from normal (control) and PRKAR2A knockout (KO) HeLa cells, using Protein Kinase A Regulatory Subunit II alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of L929 cells using [KO Validated] PRKAR2A Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Protein Kinase A Regulatory Subunit II alpha antibody](http://images.novusbio.com/fullsize/Protein-Kinase-A-Regulatory-Subunit-II-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03477-img0001.jpg)
![Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-03477] - Analysis of extracts of various cell lines, using Protein Kinase A Regulatory Subunit II alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Protein-Kinase-A-Regulatory-Subunit-II-alpha-Antibody-Western-Blot-NBP3-03477-img0003.jpg)
![Immunohistochemistry: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] - Image of Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.](http://images.novusbio.com/fullsize/nbp3-26369_rabbit-protein-kinase-a-regulatory-subunit-ii-alpha-mab-262202415475647.jpg "Immunohistochemistry: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] - Image of Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.")
![Immunohistochemistry: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] - Image of Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.](http://images.novusbio.com/fullsize/nbp3-26369_rabbit-protein-kinase-a-regulatory-subunit-ii-alpha-mab-262202415475627.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] - Staining of Hela with Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 512-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26369_rabbit-protein-kinase-a-regulatory-subunit-ii-alpha-mab-282202412493647.jpg)
![Flow Cytometry: Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) [NBP3-26369] - Overlay Peak curve showing MCF-7 cells stained with Protein Kinase A Regulatory Subunit II alpha Antibody (15G8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26369_rabbit-protein-kinase-a-regulatory-subunit-ii-alpha-mab-282202414183669.jpg)
![Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-09404] - Western blot analysis of Protein Kinase A Regulatory Subunit II alpha in Human PC-3 Whole Cell lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/Protein-Kinase-A-Regulatory-Subunit-II-alpha-Antibody-Western-Blot-NBP3-09404-img0001.jpg "Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody [NBP3-09404] - Western blot analysis of Protein Kinase A Regulatory Subunit II alpha in Human PC-3 Whole Cell lysates. Antibody dilution at 1ug/ml")
![Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody (9C6B3) [NBP3-16292] - Western blot analysis of extracts of various cell lines, using Protein Kinase A Regulatory Subunit II alpha Rabbit mAb (NBP3-16292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Protein-Kinase-A-Regulatory-Subunit-II-alpha-Antibody-9C6B3-Western-Blot-NBP3-16292-img0001.jpg "Western Blot: Protein Kinase A Regulatory Subunit II alpha Antibody (9C6B3) [NBP3-16292] - Western blot analysis of extracts of various cell lines, using Protein Kinase A Regulatory Subunit II alpha Rabbit mAb (NBP3-16292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")