CrkL Antibodies
We offer CrkL Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each CrkL Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CrkL Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the CrkL Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CrkL polyclonal antibodies and browse our CrkL monoclonal antibody catalog.
Alternate Names for CrkL Antibodies
anti-CrkL antibody, anti-crk-like protein antibody, anti-v-crk avian sarcoma virus CT10 oncogene homolog-like antibody, anti-v-crk sarcoma virus CT10 oncogene homolog (avian)-like antibody
16 Results for "crkl" in Primary Antibodies
![Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody (M03), clone 4B5. Lane 1: CRKL transfected lysatE (33.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Western-Blot-H00001399-M03-img0008.jpg "Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody (M03), clone 4B5. Lane 1: CRKL transfected lysatE (33.8 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between PTK2 and CRKL. Huh7 cells were stained with anti-PTK2 rabbit purified polyclonal 1:600 and anti-CRKL mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction com](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Immunocytochemistry-Immunofluorescence-H00001399-M03-img0006.jpg)
![Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi or non-transfected control. Blot probed with H00001399-M03. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Western-Blot-H00001399-M03-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of monoclonal antibody to CRKL on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Immunocytochemistry-Immunofluorescence-H00001399-M03-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between GAB1 and CRKL. HeLa cells were stained with anti-GAB1 rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Immunocytochemistry-Immunofluorescence-H00001399-M03-img0005.jpg)
![ELISA: CrkL Antibody (4B5) [H00001399-M03] - Detection limit for recombinant GST tagged CRKL is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-ELISA-H00001399-M03-img0003.jpg)
![Proximity Ligation Assay: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between HCK and CRKL. Mahlavu cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction co](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Proximity-Ligation-Assay-H00001399-M03-img0007.jpg)
![Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884] - Paraffin embedded human thymus. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CrkL-Antibody-Immunohistochemistry-Paraffin-NB300-884-img0001.jpg "Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884] - Paraffin embedded human thymus. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: CrkL Antibody [NB300-884] - (0.1ug/ml) staining of K562 (A) and (1ug/ml) Daudi (B) and NIH3T3 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb300-884_goat-polyclonal-crkl-antibody-1652023918333.jpg)
![Flow Cytometry: CrkL Antibody [NB300-884] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb300-884_goat-polyclonal-crkl-antibody-1652023918337.jpg)
![Immunocytochemistry/Immunofluorescence: CrkL Antibody [NB300-884] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb300-884_goat-polyclonal-crkl-antibody-16520239183315.jpg)
![Western Blot: CrkL Antibody (JE50-21) [NBP3-32222] - Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (NBP3-32222) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: A431 cell lysate (20 ug/Lane) Lane 3: HT-29 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: NIH/3T3 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32222) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32222_rabbit-crkl-mab-je50-21-278202416285269.jpg "Western Blot: CrkL Antibody (JE50-21) [NBP3-32222] - Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (NBP3-32222) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: A431 cell lysate (20 ug/Lane) Lane 3: HT-29 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: NIH/3T3 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32222) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CrkL Antibody (JE50-21) [NBP3-32222] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CrkL antibody (NBP3-32222) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32222) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32222_rabbit-crkl-mab-je50-21-298202414333684.jpg)
![Flow Cytometry: CrkL Antibody (JE50-21) [NBP3-32222] - Flow cytometric analysis of A431 cells labeling CrkL. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32222, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32222_rabbit-crkl-mab-je50-21-392024884662.jpg)
![Western Blot: CrkL Antibody [NBP1-86181] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).](http://images.novusbio.com/fullsize/CrkL-Antibody-Western-Blot-NBP1-86181-img0005.jpg "Western Blot: CrkL Antibody [NBP1-86181] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).")
![Immunohistochemistry-Paraffin: CrkL Antibody [NBP1-86181] - Staining of human fallopian tube shows membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CrkL-Antibody-Immunohistochemistry-Paraffin-NBP1-86181-img0006.jpg)
![Western Blot: CrkL Antibody [NBP1-86181] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-135](http://images.novusbio.com/fullsize/CrkL-Antibody-Western-Blot-NBP1-86181-img0004.jpg)
![Western Blot: CrkL Antibody (GT466) [NBP3-13625] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody [GT466] (NBP3-13625) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CrkL-Antibody-GT466-Western-Blot-NBP3-13625-img0001.jpg "Western Blot: CrkL Antibody (GT466) [NBP3-13625] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody [GT466] (NBP3-13625) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625] - CrkL antibody [GT466] detects CrkL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CrkL stained by CrkL antibody [GT466] (NBP3-13625) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CrkL-Antibody-GT466-Immunohistochemistry-Paraffin-NBP3-13625-img0003.jpg)
![Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625] - CrkL antibody [GT466] detects CrkL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. CrkL stained by CrkL antibody [GT466] (NBP3-13625) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CrkL-Antibody-GT466-Immunohistochemistry-Paraffin-NBP3-13625-img0002.jpg)
![Western Blot: CrkL [p Tyr207] Antibody [NBP3-13318] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (NBP3-13318) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CrkL-[p-Tyr207]-Antibody-Western-Blot-NBP3-13318-img0003.jpg "Western Blot: CrkL [p Tyr207] Antibody [NBP3-13318] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (NBP3-13318) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: CrkL [p Tyr207] Antibody [NBP3-13318] - Untreated (-) and treated (+) K562 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (NBP3-13318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CrkL-[p-Tyr207]-Antibody-Western-Blot-NBP3-13318-img0001.jpg)
![Western Blot: CrkL [p Tyr207] Antibody [NBP3-13318] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (NBP3-13318) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CrkL-[p-Tyr207]-Antibody-Western-Blot-NBP3-13318-img0002.jpg)
![Western Blot: CrkL Antibody [NBP2-55130] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/CrkL-Antibody-Western-Blot-NBP2-55130-img0003.jpg "Western Blot: CrkL Antibody [NBP2-55130] - Analysis in human cell line K562.")
![Immunocytochemistry/Immunofluorescence: CrkL Antibody [NBP2-55130] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CrkL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55130-img0001.jpg)
![Western Blot: CrkL [p Tyr207] Antibody [NBP3-23257] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23257_rabbit-crkl-p-tyr207-pab-199202312403715.jpg "Western Blot: CrkL [p Tyr207] Antibody [NBP3-23257] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).")
![Western Blot: CrkL Antibody [NBP3-23256] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23256_rabbit-crkl-pab-1992023191547.jpg "Western Blot: CrkL Antibody [NBP3-23256] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).")