BAK Antibodies
We offer BAK Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each BAK Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our BAK Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the BAK Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our BAK polyclonal antibodies and browse our BAK monoclonal antibody catalog.
Alternate Names for BAK Antibodies
anti-BAK antibody, anti-BAK1 antibody, anti-Apoptosis regulator BAK antibody, anti-BAK-LIKE antibody, anti-BAKMGC3887 antibody, anti-Bcl-2-like protein 7 antibody, anti-BCL2-antagonist/killer 1 antibody, anti-bcl2-L-7 antibody, anti-BCL2-like 7 protein antibody, anti-BCL2L7bcl-2 homologous antagonist/killer antibody, anti-CDN1 antibody, anti-MGC117255 antibody, anti-pro-apoptotic protein BAK antibody
9 Results for "bak" in Primary Antibodies
![Western Blot: BAK Antibody (AT38E2) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : Jurkat cell lysateLane 6 : MCF7 cell lysateLane 7 : PC3 cell lysate](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Western-Blot-NBP1-74026-img0009.jpg "Western Blot: BAK Antibody (AT38E2) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : Jurkat cell lysateLane 6 : MCF7 cell lysateLane 7 : PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HeLa antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Immunocytochemistry-Immunofluorescence-NBP1-74026-img0006.jpg)
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0011.jpg)
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0007.jpg)
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on RAW 246.7 cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0010.jpg)
![Immunoprecipitation: BAK Antibody (8D1) [NBP3-26482] - Immunoprecipitating BAK in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26482 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26482 (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-262202413221514.jpg "Immunoprecipitation: BAK Antibody (8D1) [NBP3-26482] - Immunoprecipitating BAK in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26482 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26482 (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).")
![Immunohistochemistry: BAK Antibody (8D1) [NBP3-26482] - Image of BAK Antibody (8D1) diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-2722024840450.jpg)
![Immunohistochemistry: BAK Antibody (8D1) [NBP3-26482] - Image of BAK Antibody (8D1) diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-27220248573315.jpg)
![Western Blot: BAK Antibody (8D1) [NBP3-26482] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Colo320 whole cell lysate. All lanes: BAK Antibody at 0.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 24, 17 KDa Observed band size: 24 KDa](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-28220241140912.jpg)
![Flow Cytometry: BAK Antibody (8D1) [NBP3-26482] - Overlay histogram showing Hela cells stained with BAK Antibody (8D1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-28220241418365.jpg)
![Western Blot: BAK Antibody [NBP1-77152] - Analysis of Bak in L1210 cell lysates with Bak antibody using NBP1-77152 at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/BAK-Antibody-Western-Blot-NBP1-77152-img0003.jpg "Western Blot: BAK Antibody [NBP1-77152] - Analysis of Bak in L1210 cell lysates with Bak antibody using NBP1-77152 at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: BAK Antibody [NBP1-77152] - Staining of L1210 cells at 2 ug/mL.](http://images.novusbio.com/fullsize/BAK-Antibody-Immunocytochemistry-NBP1-77152-img0004.jpg)
![Western Blot: BAK Antibody [NB100-893] - Staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). BAK antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAK-Antibody-Western-Blot-NB100-893-img0001.jpg "Western Blot: BAK Antibody [NB100-893] - Staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). BAK antibody at 2 ug/mL. Detected by chemiluminescence.")
![Western Blot: BAK Antibody (3K5Y9) [NBP3-15336] - Western blot analysis of extracts of various cell lines, using (NBP3-15336) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/BAK-Antibody-3K5Y9-Western-Blot-NBP3-15336-img0001.jpg "Western Blot: BAK Antibody (3K5Y9) [NBP3-15336] - Western blot analysis of extracts of various cell lines, using (NBP3-15336) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")