B-Raf Antibodies
We offer B-Raf Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each B-Raf Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our B-Raf Antibodies can be used in a variety of model species: Human, Monkey, Mouse, Rat. Use the list below to choose the B-Raf Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our B-Raf polyclonal antibodies and browse our B-Raf monoclonal antibody catalog.
Alternate Names for B-Raf Antibodies
anti-B-Raf antibody, anti-BRAF antibody, anti-94 kDa B-raf protein antibody, anti-B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody, anti-B-RAF1 antibody, anti-BRAF1MGC126806 antibody, anti-EC 2.7.11.1 antibody, anti-MGC138284 antibody, anti-murine sarcoma viral (v-raf) oncogene homolog B1 antibody, anti-NS7 antibody, anti-p94 antibody, anti-Proto-oncogene B-Raf antibody, anti-RAFB1 antibody, anti-serine/threonine-protein kinase B-raf antibody, anti-v-raf murine sarcoma viral oncogene homolog B1FLJ95109 antibody
16 Results for "b-raf" in Primary Antibodies
![Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668] - Analysis of extracts (35ug) from 9 different cell lines by using anti-B Raf monoclonal antibody.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Western-Blot-NBP1-47668-img0019.jpg "Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668] - Analysis of extracts (35ug) from 9 different cell lines by using anti-B Raf monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY B Raf.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Immunocytochemistry-Immunofluorescence-NBP1-47668-img0013.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human pancreas tissue using anti-B Raf mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Immunohistochemistry-Paraffin-NBP1-47668-img0018.jpg)
![Flow Cytometry: B-Raf Antibody (OTI5A9) [NBP1-47668] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-B Raf antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Flow-Cytometry-NBP1-47668-img0012.jpg)
![Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antibody](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Western-Blot-NBP1-47668-img0011.jpg)
![Immunohistochemistry: B-Raf Antibody (OTI5A9) [NBP1-47668] - The images depict immunostaining of representative PTC cases harboring BRAF wild-type (A, B) and BRAF V600E mutation (C, D) proven by NGS; varying degrees of immunoreactivity for BRAF is shown both in the intranuclear inclusions (arrows) and in the cytoplasm. Original magnifications: 1,000 X.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Immunohistochemistry-NBP1-47668-img0020.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-B Raf mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Immunohistochemistry-Paraffin-NBP1-47668-img0014.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-B Raf mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Immunohistochemistry-Paraffin-NBP1-47668-img0015.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human endometrium tissue using anti-B Raf mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Immunohistochemistry-Paraffin-NBP1-47668-img0016.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human lung tissue using anti-B Raf mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Immunohistochemistry-Paraffin-NBP1-47668-img0017.jpg)
![Immunoprecipitation: B-Raf Antibody (OTI5A9) [NBP1-47668] - (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/B-Raf-Antibody-OTI5A9-Immunoprecipitation-NBP1-47668-img0021.jpg)
![Western Blot: B-Raf Antibody [NBP1-89942] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP1-89942-img0015.jpg "Western Blot: B-Raf Antibody [NBP1-89942] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunohistochemistry-Paraffin-NBP1-89942-img0021.jpg)
![Western Blot: B-Raf Antibody [NBP1-89942] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP1-89942-img0014.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunohistochemistry-Paraffin-NBP1-89942-img0016.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunohistochemistry-Paraffin-NBP1-89942-img0017.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human kidney shows moderate cytoplasmic positivity in distal tubules.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunohistochemistry-Paraffin-NBP1-89942-img0018.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunohistochemistry-Paraffin-NBP1-89942-img0020.jpg)
![Simple Western: B-Raf Antibody [NBP1-89942] - Simple Western lane view shows a specific band for BRAF in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Simple-Western-NBP1-89942-img0008.jpg)
![Simple Western: B-Raf Antibody [NBP1-89942] - Electropherogram image(s) of corresponding Simple Western lane view. B-Raf antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/B-Raf-Antibody-Simple-Western-NBP1-89942-img0009.jpg)
![Western Blot: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein using NBP2-61503 at 2 ug/ml.](http://images.novusbio.com/fullsize/B-Raf-Antibody-RM8-V600-E-Mutation-Western-Blot-NBP2-61503-img0011.jpg "Western Blot: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein using NBP2-61503 at 2 ug/ml.")
![Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections using NBP2-61503 at 2 ug/ml](http://images.novusbio.com/fullsize/B-Raf-Antibody-RM8-V600-E-Mutation-Immunohistochemistry-Paraffin-NBP2-61503-img0004.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Immunohistochemical staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue. Allele specific PCR validated to be Negative for BRAF V600E.](http://images.novusbio.com/fullsize/B-Raf-Antibody-RM8-V600-E-Mutation-Immunohistochemistry-Paraffin-NBP2-61503-img0009.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Staining of formalin fixed and paraffin embedded human cell line sections (SW40 cells at 400X, negative)](http://images.novusbio.com/fullsize/B-Raf-Antibody-RM8-V600-E-Mutation-Immunohistochemistry-Paraffin-NBP2-61503-img0010.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Staining of formalin fixed and paraffin embedded human cell line sections (WiDr Cells, 400x, Positive)](http://images.novusbio.com/fullsize/B-Raf-Antibody-RM8-V600-E-Mutation-Immunohistochemistry-Paraffin-NBP2-61503-img0001.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue. Allele specific PCR validated to be Positive for BRAF V600E.](http://images.novusbio.com/fullsize/B-Raf-Antibody-RM8-V600-E-Mutation-Immunohistochemistry-Paraffin-NBP2-61503-img0003.jpg)
![Western Blot: B-Raf Antibody [NBP2-15531] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B Raf antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP2-15531-img0007.jpg "Western Blot: B-Raf Antibody [NBP2-15531] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B Raf antibody diluted by 1:1000.")
![Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-15531] - Paraffin-embedded mouse testis. B-Raf antibody [N2C1], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunohistochemistry-Paraffin-NBP2-15531-img0008.jpg)
![Western Blot: B-Raf Antibody [NBP2-15531] - A. 30 ug PC-12 whole cell extract 7.5 % SDS-PAGE B-Raf antibody [N2C1], Internal dilution: 1:5000](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP2-15531-img0002.jpg)
![Western Blot: B-Raf Antibody [NBP2-15531] - Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal diluted at 1:5000.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP2-15531-img0006.jpg)
![Immunoprecipitation: B-Raf Antibody [NBP2-15531] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 ug of B-Raf antibody [N2C1], Internal 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunoprecipitation-NBP2-15531-img0004.jpg)
![Western Blot: B-Raf Antibody [NBP2-41219] - 15 ug of lysates per lane. Antibodies: B-raf 5777, (0.5 ug/mL), B-raf 7379, (0.5 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antichicken and goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP2-41219-img0004.jpg "Western Blot: B-Raf Antibody [NBP2-41219] - 15 ug of lysates per lane. Antibodies: B-raf 5777, (0.5 ug/mL), B-raf 7379, (0.5 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antichicken and goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/Immunofluorescence: B-Raf Antibody - BSA Free [NBP2-41219] - B-Raf Antibody [NBP2-41219] - B-Raf in human small intestine tissue with B-raf antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunofluorescence-NBP2-41219-img0003.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-41219] - Human small intestine tissue with B-raf antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunohistochemistry-Paraffin-NBP2-41219-img0002.jpg)
![Western Blot: B-Raf Antibody [NBP2-41219] - Analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP2-41219-img0001.jpg)
![Western Blot: B-Raf Antibody (CL4003) [NBP2-59034] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4003-Western-Blot-NBP2-59034-img0005.jpg "Western Blot: B-Raf Antibody (CL4003) [NBP2-59034] - Analysis in human cell line MOLT-4.")
![Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human breast cancer shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4003-Immunohistochemistry-Paraffin-NBP2-59034-img0004.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4003-Immunohistochemistry-Paraffin-NBP2-59034-img0001.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human cerebral cortex shows moderate positivity in neuropil and cytoplasmic staining in some neurons.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4003-Immunohistochemistry-Paraffin-NBP2-59034-img0002.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4003-Immunohistochemistry-Paraffin-NBP2-59034-img0003.jpg)
![Western Blot: B-Raf Antibody (CL4004) [NBP2-59035] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4004-Western-Blot-NBP2-59035-img0005.jpg "Western Blot: B-Raf Antibody (CL4004) [NBP2-59035] - Analysis in human cell line MOLT-4.")
![Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human testis shows cytoplasmic positivity in seminiferous tubules.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4004-Immunohistochemistry-Paraffin-NBP2-59035-img0004.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human prostate cancer shows moderate cytoplasmic immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4004-Immunohistochemistry-Paraffin-NBP2-59035-img0001.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4004-Immunohistochemistry-Paraffin-NBP2-59035-img0002.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human breast cancer shows moderate cytoplasmic immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/B-Raf-Antibody-CL4004-Immunohistochemistry-Paraffin-NBP2-59035-img0003.jpg)
![Western Blot: B-Raf Antibody (1H12) [NBP1-47433] - Analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.](http://images.novusbio.com/fullsize/B-Raf-Antibody-1H12-Western-Blot-NBP1-47433-img0010.jpg "Western Blot: B-Raf Antibody (1H12) [NBP1-47433] - Analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.")
![Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) [NBP1-47433] - Analysis of human testis tissues using BRAF mouse mAb.](http://images.novusbio.com/fullsize/B-Raf-Antibody-1H12-Immunohistochemistry-Paraffin-NBP1-47433-img0009.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) [NBP1-47433] - Analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye](http://images.novusbio.com/fullsize/B-Raf-Antibody-1H12-Immunohistochemistry-Paraffin-NBP1-47433-img0008.jpg)
![Western Blot: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis using BRAF mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).](http://images.novusbio.com/fullsize/B-Raf-Antibody-1H12F1-Western-Blot-NBP1-47555-img0004.jpg "Western Blot: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis using BRAF mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).")
![Immunohistochemistry-Paraffin: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis of human bladder carcinoma tissue(left) and lung carcinoma tissue (right) showing cytoplasmic localization using BRAF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/B-Raf-Antibody-1H12F1-Immunohistochemistry-Paraffin-NBP1-47555-img0005.jpg)
![Western Blot: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis using BRAF mouse mAb against truncated recombinant Braf (1) and A431 cell lysate (2).](http://images.novusbio.com/fullsize/B-Raf-Antibody-1H12F1-Western-Blot-NBP1-47555-img0003.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis of human testis tissues using BRAF mouse mAb.](http://images.novusbio.com/fullsize/B-Raf-Antibody-1H12F1-Immunohistochemistry-Paraffin-NBP1-47555-img0001.jpg)
![Western Blot: B-Raf Antibody [NBP2-58818] - Analysis in human cell line BEWO.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Western-Blot-NBP2-58818-img0003.jpg "Western Blot: B-Raf Antibody [NBP2-58818] - Analysis in human cell line BEWO.")
![Immunocytochemistry/Immunofluorescence: B-Raf Antibody [NBP2-58818] - Staining of human cell line SiHa shows localization to vesicles.](http://images.novusbio.com/fullsize/B-Raf-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58818-img0001.jpg)