AIF Antibodies
We offer AIF Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each AIF Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our AIF Antibodies can be used in a variety of model species: Bovine, Canine, Human, Mouse, Primate, Rat. Use the list below to choose the AIF Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our AIF polyclonal antibodies and browse our AIF monoclonal antibody catalog.
Alternate Names for AIF Antibodies
anti-AIF antibody, anti-AIFM1 antibody, anti-AIFapoptosis-inducing factor 1, mitochondrial antibody, anti-apoptosis-inducing factor, mitochondrion-associated, 1 antibody, anti-COXPD6 antibody, anti-MGC111425 antibody, anti-PDCD8striatal apoptosis-inducing factor antibody, anti-programmed cell death 8 (apoptosis-inducing factor) antibody, anti-Programmed cell death protein 8 antibody
15 Results for "aif" in Primary Antibodies
![Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Western-Blot-NBP2-37577-img0003.jpg "Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody (4E7) [NBP2-37577] - Analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunocytochemistry-Immunofluorescence-NBP2-37577-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human lung cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0009.jpg)
![Flow Cytometry: AIF Antibody (4E7) [NBP2-37577] - Analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Flow-Cytometry-NBP2-37577-img0005.jpg)
![Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Western-Blot-NBP2-37577-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human breast cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human cervical cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human rectum cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0008.jpg)
![ELISA: AIF Antibody (4E7) [NBP2-37577] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-ELISA-NBP2-37577-img0001.jpg)
![Knockout Validated: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/AIF-Antibody-Knockout-Validated-NBP2-27177-img0005.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Analysis of AIF in human colon using this antibody. Lane 1, normal colon. Lanes 2-7, colon carcinoma tissue lysatesfrom 6 different patients. mAIF, thought to represent mitochondrial AIF.](http://images.novusbio.com/fullsize/AIF-Antibody-Western-Blot-NBP2-27177-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-27177] - Staining of AIF in formalin-fixed, paraffin-embedded normal human colon using this antibody at 1:2000. Hematoxylin-eosin counterstain. In this example, AIF expression is predominant in the upper part of the crypt.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-27177-img0002.jpg)
![Simple Western: AIF Antibody [NBP2-27177] - Simple Western lane view shows a specific band for AIF in 0.2 mg/ml of Jurkat and MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/AIF-Antibody-Simple-Western-NBP2-27177-img0003.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0009.jpg "Western Blot: AIF Antibody [NBP2-42496] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42496-img0008.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42496] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42496-img0016.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0004.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A) and Rat (B) Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0005.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42496-img0007.jpg)
![Western Blot: AIF Antibody [NBP2-42509] - Staining of Jurkat lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42509-img0003.jpg "Western Blot: AIF Antibody [NBP2-42509] - Staining of Jurkat lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42509] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42509-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42509-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42509] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42509-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509] - Staining of paraffin embedded Human Kidney with antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42509-img0005.jpg)
![Western Blot: AIF Antibody [NBP2-33932] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-33932-img0002.jpg "Western Blot: AIF Antibody [NBP2-33932] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-33932] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunohistochemistry-Paraffin-NBP2-33932-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-33932] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunohistochemistry-Paraffin-NBP2-33932-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF Antibody [NBP2-33932] - Staining in human kidney and cerebral cortex tissues using anti-AIFM1 antibody. Corresponding AIFM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunohistochemistry-Paraffin-NBP2-33932-img0004.jpg)
![Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] - Image of AIF Antibody (4B2) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26515_rabbit-aif-mab-4b2-262202415253322.jpg "Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] - Image of AIF Antibody (4B2) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] - Image of AIF Antibody (4B2) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26515_rabbit-aif-mab-4b2-26220241522419.jpg)
![Western Blot: AIF Antibody (4B2) [NBP3-26515] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Rat liver tissue, Rat kidney tissue. All lanes: AIF Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 67, 36, 29, 27 kDa Observed band size: 67 kDa](http://images.novusbio.com/fullsize/nbp3-26515_rabbit-aif-mab-4b2-282202411363023.jpg)
![Western Blot: AIF Antibody (5F0Y1) [NBP3-15759] - Western blot analysis of extracts of various cell lines, using AIF antibody (NBP3-15759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AIF-Antibody-5F0Y1-Western-Blot-NBP3-15759-img0002.jpg "Western Blot: AIF Antibody (5F0Y1) [NBP3-15759] - Western blot analysis of extracts of various cell lines, using AIF antibody (NBP3-15759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: AIF Antibody (5F0Y1) [NBP3-15759] - Immunohistochemistry of paraffin-embedded human oophoroma using AIF antibody (NBP3-15759) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AIF-Antibody-5F0Y1-Immunohistochemistry-Paraffin-NBP3-15759-img0001.jpg)
![Western Blot: AIF Antibody (22E9) [NBP2-59432] - Lane 1: Mouse heart tissue lysates, Lane 2: Mouse liver tissue lysates](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-22E9-Western-Blot-NBP2-59432-img0001.jpg "Western Blot: AIF Antibody (22E9) [NBP2-59432] - Lane 1: Mouse heart tissue lysates, Lane 2: Mouse liver tissue lysates")
![Immunocytochemistry/Immunofluorescence: AIF Antibody (22E9) [NBP2-59432] - Analysis of AIFM1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AIFM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-22E9-Immunocytochemistry-Immunofluorescence-NBP2-59432-img0002.jpg)
![Western Blot: AIF Antibody (3C11) [NBP2-50606] - Western blot was performed on transfected 293T cell lysates using anti-AIF [3C11].](http://images.novusbio.com/fullsize/AIF-Antibody-3C11-Western-Blot-NBP2-50606-img0001.jpg "Western Blot: AIF Antibody (3C11) [NBP2-50606] - Western blot was performed on transfected 293T cell lysates using anti-AIF [3C11].")
![Western Blot: AIF [p Ser116] Antibody [NBP3-23346] - Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIF (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIF (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23346_rabbit-aif-p-ser116-pab-1992023191546.jpg "Western Blot: AIF [p Ser116] Antibody [NBP3-23346] - Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIF (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIF (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).")