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Vascular Smooth Muscle Cell Differentiation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Vascular Smooth Muscle Cell Differentiation Pathway and Atherosclerosis, Anaplasia, Hypertensive Disease, Arteriosclerosis, Hyperplasia. The study of the Vascular Smooth Muscle Cell Differentiation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Vascular Smooth Muscle Cell Differentiation Pathway has been researched in relation to Muscle Cell Differentiation, Cell Differentiation, Smooth Muscle Cell Differentiation, Pathogenesis, Dedifferentiation. The Vascular Smooth Muscle Cell Differentiation Pathway complements our catalog of research reagents including antibodies and ELISA kits against SM2, ALPHA-SMOOTH MUSCLE ACTIN, SMEMB, SMOOTH MUSCLE MYOSIN HEAVY CHAIN, SM22ALPHA.

Top Research Reagents

We have 1485 products for the study of the Vascular Smooth Muscle Cell Differentiation Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Transgelin/TAGLN/SM22 alpha Antibody
NB600-507
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of Human Testes lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of paraffin embedded Human Prostate. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

     1 Review

23 Publications
TOR/mTOR [p Ser2448] Antibody
NB600-607
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - affinity purified TOR/TOR/mTOR [p Ser2448] antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ELISA, IHC

     2 Reviews

9 Publications
MHC Class I Antibody (OX18) - BSA Free
NB120-6405
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY license.Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.

Mouse Monoclonal
Species Rat
Applications EM, ELISA, Flow

33 Publications
ZEB1 Antibody
NBP1-05987
Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZEB1 antibody (NBP1-05987) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, GS

     6 Reviews

97 Publications
Histone Deacetylase 2/HDAC2 Antibody
NBP1-87109
Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Analysis in mouse cell line NIH-3T3.Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

1 Publication
AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
AF887
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (<a class=Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

49 Publications
Clusterin/APOJ Antibody [Unconjugated]
AF2747
Clusterin was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=Clusterin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Goat Polyclonal
Species Mouse
Applications WB, IHC, IP

13 Publications
Myocardin Antibody (355521) [Unconjugated]
MAB4028
Western blot shows lysates of MCF-7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Laminin-111 but not laminin-alpha 4 blocking antibody affects pericyte differentiation. (a) Immunoblots show that laminin-111 blockage (Ln Ab) significantly enhances the expression of PDGFR beta , SMA, and SM22-alpha , but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14b. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=5–6). (b) Immunoblots show that laminin-alpha 4 blockage (Anti-Ln alpha 4) does not change the expression of PDGFR beta , SMA, SM22-alpha , or myocardinin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14c. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=3). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse Monoclonal
Species Human, Mouse
Applications WB

15 Publications
Myosin Heavy Chain Antibody (MF20) [Unconjugated]
MAB4470
Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class= Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Mouse Monoclonal
Species Multi-Species
Applications WB, IHC, ICC

     1 Review

162 Publications
KLF4 Antibody [Unconjugated]
AF3158
D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) or control antibody (Catalog # <A class=NoLineLink href=KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

Goat Polyclonal
Species Mouse
Applications WB, IHC, ChIP

     2 Reviews

87 Publications
AE Binding Protein 1/ACLP Antibody (590831) [Unconjugated]
MAB6425
HASMC human human aortic smooth muscle cells was stained with Human ACLP Monoclonal Antibody (Catalog # MAB6425, filled histogram) or isotype control antibody (Catalog # <A class=NoLineLink href=Molt-4 human human cell line was stained with Human ACLP Monoclonal Antibody (Catalog # MAB6425, filled histogram) or isotype control antibody (<a class=NoLineLink href=

Mouse Monoclonal
Species Human
Applications CyTOF-ready, ICFlow

Smoothelin Antibody
NBP2-37931
Immunohistochemistry-Paraffin: Smoothelin Antibody [NBP2-37931] - Staining in human prostate and liver tissues using NBP2-37931 antibody. Corresponding SMTN RNA-seq data are presented for the same tissues.Western Blot: Smoothelin Antibody [NBP2-37931] - Analysis in human cell lines PC-3 and HeLa using anti-SMTN antibody. Corresponding SMTN RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

4 Publications
PTGIR Antibody - BSA Free
NBP2-93654
Western Blot: PTGIR Antibody [NBP2-93654] - Analysis of extracts of various cell lines, using PTGIR . Exposure time: 90s.Immunohistochemistry-Paraffin: PTGIR Antibody [NBP2-93654] - Paraffin-embedded human stomach using PTGIR .

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

1 Publication
Serum Response Factor SRF Antibody (PCRP-SRF-1F7)
NBP3-13785
Immunocytochemistry/Immunofluorescence: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Serum Response Factor SRF Antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using Serum Response Factor SRF antibody (PCRP-SRF-1F7) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow