We are using the Transgelin antibody (NB600-507) for western analysis of mouse and human uterine tissue. In the mouse we see a single band of 22 kDa but in the human we see a band of 12 kDa. Any thoughts on this? I can not find any information on a smaller transgelin band.

Name: Transgelin/TAGLN/SM22 alpha Antibody
Brand: Novus Biologicals
SKU: NB600-507
Rating: 5 (1 reviews)

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Accepted Answer

I have searched for an explanation for the lower molecular weight band you are detecting, and have found that this paper, PMC2789179 says: 'It should be noted that the expression analysis of TAGLN is complicated by the fact that multiple bands were detected on the Western blots. Similar observations have been described and attributed to C-terminal proteolysis. While the sum of band intensities was used for the analyses here, it is possible that TAGLN regulation is not a simple change in quantity of one defined species, but may, in fact, be subject to posttranslationally regulated events such as modification or degradation.'

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications	WB, ELISA, IHC, KD
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	0.5 mg/ml

Immunogen	Peptide with sequence C-MTGYGRPRQIIS corresponding to C-Terminus according to NP_003177.2.
Specificity	NP_003177.2 and NP_001001522.1 represent identical protein.The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumor suppressor.
Predicted Species	Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	TAGLN
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative	0.02% Sodium Azide
Concentration	0.5 mg/ml
Purity	Immunogen affinity purified

Publications using NB600-507	Applications	Species
PUtz S, Barthel LS, Frohn M et al. Caldesmon ablation in mice causes umbilical herniation and alters contractility of fetal urinary bladder smooth muscle The Journal of general physiology 2021-07-05 [PMID: 34115104]
Li J, Liu H, Takagi S et Al. Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules JCI Insight 2020-03-26 [PMID: 32134397] (WB, KD, Mouse)	WB, KD	Mouse
Takagaki Y, Lee SM, Dongqing Z et al. Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis Autophagy 2020-01-22 [PMID: 31965901] (WB, IF/IHC)	WB, IF/IHC
Deng JT, Bhaidani S, Sutherland C Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells PLoS ONE 2019-12-13 [PMID: 31834925] (WB, Human)	WB	Human
Hong K, Zhao G, Hong Z et al. Mechanical activation of angiotensin II type 1 receptors causes actin remodelling and myogenic responsiveness in skeletal muscle arterioles. J. Physiol. (Lond.). 2016-08-17 [PMID: 27531064] (WB, Rat)	WB	Rat
Herencia C, Diaz-Tocados JM, Jurado L et al. Procaine Inhibits Osteo/Odontogenesis through Wnt/b-Catenin Inactivation. PLoS ONE 2016-06-04 [PMID: 27257912]
Thibodeau JF, Holterman CE, He Y et al. Vascular smooth muscle-specific EP4 receptor deletion in mice exacerbates angiotensin II-induced renal injury. Antioxid. Redox Signal. 2016-06-01 [PMID: 27245461]
Abd-Elrahman KS, Colinas O, Walsh EJ et al. Abnormal myosin phosphatase targeting subunit 1 phosphorylation and actin polymerization contribute to impaired myogenic regulation of cerebral arterial diameter in the type 2 diabetic Goto-Kakizaki rat. J. Cereb. Blood Flow Metab. 2015-12-31 [PMID: 26721393] (WB, Rat)	WB	Rat
Colinas O, Moreno-Dominguez A, Zhu HL et al. alpha5-Integrin-mediated cellular signaling contributes to the myogenic response of cerebral resistance arteries. Biochem. Pharmacol. 2015-08-14 [PMID: 26278977] (WB, Rat)	WB	Rat
El-Yazbi AF, Abd-Elrahman KS, Moreno-Dominguez A. PKC-mediated cerebral vasoconstriction: role of myosin light chain phosphorylation versus actin cytoskeleton reorganization Biochem. Pharmacol. 2015-04-27 [PMID: 25931148] (WB, Rat)	WB	Rat
Deng JT, Wang XL, Chen YX et al. The effects of knockdown of rho-associated kinase 1 and zipper-interacting protein kinase on gene expression and function in cultured human arterial smooth muscle cells PLoS ONE 2015-02-28 [PMID: 25723491] (WB, Human) Details: goat anti-SM-22 antibody used at 1:2500 dilution in WB application for analysis of Transgelin/TAGLN/SM22 alpha protein in lysates of control human Umbilical Artery Smooth Muscle Cells/UASMCs and UASMC 48 h after transfection with siRNAs to ZIPK or ROCK1. SM22 in this assay was used as loading control for the quantitative analysis of IL1 Beta protein (Fig 4A).	WB	Human
Hovater MB, Ying WZ, Agarwal A, Sanders PW. Nitric oxide and carbon monoxide antagonize TGF-b through ligand-independent internalization of TbR1/ALK5. Am. J Physiol. Renal Physiol. 2014-08-06 [PMID: 25100282] (WB)	WB
Moreno-Dominguez A, El-Yazbi AF, Zhu HL et al. Cytoskeletal Reorganization Evoked by Rho-associated kinase- and Protein Kinase C-Catalyzed Phosphorylation of Cofilin and Heat Shock Protein 27, Respectively, Contributes to Myogenic Constriction of Rat Cerebral Arteries. J. Biol. Chem. 2014-06-09 [PMID: 24914207] (WB, Rat) Details: Antibody used for WB on RMCAs/rat middle cerebral arteries (Sprague-Dawley rats) - goat polyclonal anti-SM22 alpha (1:2000 dilution), incubated overnight at 4 C in 1% dry milk in 0.1% TBST (article has highly detailed WB method under G-actin Determination section); WB images -FIGURES 1E, 2E, 3E, 4C, 5C, 7A/B, 8A, 13A - Levels of G-actin were normalized to SM22 content, because SM22 is exclusively retained in the supernatant during high speed centrifugation and expressed as a fraction of the level at 10 mm Hg (Walshet al. 2011 Am. J. Physiol. Renal Physiol. 300, F73–F85)	WB	Rat
Ying WZ, Aaron KJ, Sanders PW. Transforming Growth Factor-beta Regulates Endothelial Function During High Salt Intake in Rats. Hypertension. 2013-11-01 [PMID: 24041947] (WB, Human)	WB	Human
Moreno-Dominguez A, Colinas O, El-Yazbi A et al. Calcium Sensitization Due to Myosin Light Chain Phosphatase Inhibition and Cytoskeletal Reorganization in The Myogenic Response of Skeletal Muscle Resistance Arteries J Physiol 2012-12-10 [PMID: 23230233] (WB, Rat)	WB	Rat
Donati C, Marseglia G, Magi A et al. Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6. [PMID: 21629665]
Walsh MP, Thornbury K, Cole WC et al. Rho-associated kinase plays a role in rabbit urethral smooth muscle contraction, but not via enhanced myosin light chain phosphorylation Am J Physiol Renal Physiol 2011-01-01 [PMID: 20861082] (WB)	WB
Montes de Oca A, Madueno JA, Martinez-Moreno JM et al. High-phosphate-induced calcification is related to SM22alpha promoter methylation in vascular smooth muscle cells. J Bone Miner Res 2010-09-01 [PMID: 20499380] (WB, Human)	WB	Human
Cencetti F, Bernacchioni C, Nincheri P et al. Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis. [PMID: 20089836]
Nincheri P, Luciani P, Squecco R et al. Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells. [PMID: 19337690]
Leguillette R, Laviolette M, Bergeron C et al. Myosin, transgelin, and myosin light chain kinase: expression and function in asthma. [PMID: 19011151]
Donoghue P, Doran P, Wynne K et al. Proteomic profiling of chronic low-frequency stimulated fast muscle. [PMID: 17708595]
Nishida W, Kitami Y, Abe M, Hiwada K. Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle. Biochem Int 1991-03-01 [PMID: 1872880]
Show All 23 Publications. Collapse Publications.

Transgelin/TAGLN/SM22 alpha Antibody

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HeLa Whole Cell Lysate
Transgelin/TAGLN/SM22 alpha Overexpression Lysate

Gene Symbol	TAGLN
Entrez	6876(Human) 21345(Mouse) 25123(Rat)
Uniprot	Q01995() NP_003177.2(Human)

	Katie Tobik 11/03/2017
Application:	IF
Species:	Human