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Oxidative Demethylation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Oxidative Demethylation Pathway and Malignant Neoplasms, Neoplasms, Obesity, Drug-induced Liver Injury, Malignant Neoplasm Of Breast. The study of the Oxidative Demethylation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Oxidative Demethylation Pathway has been researched in relation to Demethylation, Dna Repair, Conjugation, Methylation, Excretion. The Oxidative Demethylation Pathway complements our catalog of research reagents including antibodies and ELISA kits against APEX1, BRCA2, C4B, CAT, COMT.

Top Research Reagents

We have 1506 products for the study of the Oxidative Demethylation Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

FTO Antibody (2Q9K10)
NBP3-16285
Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunocytochemistry/Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Immunofluorescence analysis of U-2 OS cells using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Rabbit Monoclonal
Species Human
Applications WB, ICC/IF, IHC

Presenilin-1 Antibody (APS 18)
NB100-74510
Western Blot: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of THP-1 cell lysate.Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) shows staining in A2058 melanoma cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 at a dilution of 1:20-1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

     1 Review

5 Publications
FANCD2 Antibody - BSA Free
NB100-182
Knockdown Validated: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Effects of FANCD2 knockdown on levels of cell cycle regulators. Caki-1 cells were transfected with control siRNA (siCon) or FANCD2 siRNA (siFD2#2) and treated with AICAR for 24 h. The levels of FANCD2, p15Ink4B, and p21Cip1 were evaluated by immunoblotting. Image collected and cropped by CiteAb from the following publication (https://doi.wiley.com/10.1002/2211-5463.12185) licensed under a CC-BY license.Knockdown Validated: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Resistance of FANCA-mutant cells with defective FANCD2 function to hydrogen peroxide (H2O2). Representative images illustrating staining for subnuclear FANCD2 foci in isogenic fibroblast pairs, either deficient for FANCD2 (PD20) or FANCA (PD220) and their respective wild-type (wt) complemented counterparts. Foci were visualized three hours after treatment with H2O2 (25 uM for 2 hours). Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2008/821529/) licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     8 Reviews

228 Publications
ABH2 Antibody - BSA Free
NBP1-76276
Western Blot: ABH2 Antibody [NBP1-76276] - Human kidney tissue lysate with ALKBH2 antibody at (A) 1 and (B) 2 ug/mL.Immunohistochemistry-Paraffin: ABH2 Antibody [NBP1-76276] - ALKBH2 in human kidney tissue with ALKBH2 antibody at 10 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IHC

1 Publication
ABH1 Antibody
H00008846-B01P
Western Blot: ABH1 Antibody [H00008846-B01P] - Analysis of ALKBH1 expression in transfected 293T cell line by ALKBH1 polyclonal antibody. Lane 1: ALKBH transfected lysate(42.79 KDa). Lane 2: Non-transfected lysate.Immunocytochemistry/Immunofluorescence: ABH1 Antibody [H00008846-B01P] - Analysis of purified antibody to ALKBH on HeLa cell. (antibody concentration 10 ug/ml)

Mouse Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

1 Publication
ALKBH3 Antibody (2A5-4F5)
H00221120-M01
Western Blot: ALKBH3 Antibody (2A5-4F5) [H00221120-M01] - DEPC-1 monoclonal antibody (M01), clone 2A5-4F5 Analysis of DEPC-1 expression in K-562.Sandwich ELISA: ALKBH3 Antibody (2A5-4F5) [H00221120-M01] - Detection limit for recombinant GST tagged DEPC-1 is approximately 0.03ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications WB, ELISA, S-ELISA

CRAT Antibody
NBP1-86616
Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86616] - Analysis in human testis and lymph node tissues. Corresponding CRAT RNA-seq data are presented for the same tissues.Western Blot: CRAT Antibody [NBP1-86616] - Analysis using Anti-CRAT antibody NBP1-86616 (A) shows similar pattern to independent antibody NBP1-86615 (B).

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

2 Publications
COMT Antibody
NB100-1780
Western Blot: COMT Antibody [NB100-1780] - Staining of MCF7 (A) with antibody at 0.5 ug/mL, U251 cell lysate 1 (B) and U251 cell lysate 2 (C) (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.Flow Cytometry: COMT Antibody [NB100-1780] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Goat Polyclonal
Species Human, Mouse
Applications WB, Flow, PEP-ELISA

3 Publications
Cytochrome P450 2A6 Antibody (OTI1D2)
NBP2-01437
Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2A6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Human prostate tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IHC

DMGDH Antibody
NBP2-32666
Immunohistochemistry-Paraffin: DMGDH Antibody [NBP2-32666] - Staining of human colon.Immunohistochemistry-Paraffin: DMGDH Antibody [NBP2-32666] - Staining of human liver shows high expression.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

1 Publication
Catalase Antibody [Unconjugated]
AF3398
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC

     3 Reviews

15 Publications
BRCA2 Antibody (234403) [Unconjugated]
MAB2476
Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=BRCA2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (<a class=

Mouse Monoclonal
Species Human
Applications WB, IHC

13 Publications
Complement C4b/d Antibody (16D2)
NB200-541
Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.

Rat Monoclonal
Species Mouse
Applications WB, IA, ICC/IF

12 Publications
Cytochrome P450 3A4 Antibody (3H8) - BSA Free
NBP2-37502
Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

1 Publication
LSD1 Antibody (1B2E5)
NB100-1762
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. KDM1A protein expresion was evaluated immunohistochemically in a tissue microarray of 70 medulloblastomas (MB) and 9 tissue samples of normal cerebellum (CB). Micrograph showing KDM1A-positive staining in a representative MB sample, and KDM1A-negative staining in CB, scale bar=100AI1/4m. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. <i>Acta Neuropathol Commun</i> (2013)) licensed under a CC-BY license.

Mouse Monoclonal
Species Human, Mouse, Primate
Applications WB, Simple Western, ELISA

12 Publications
GLYAT Antibody
NBP2-58917
Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human skeletal muscle.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

Cytochrome c Antibody (7H8.2C12) - BSA Free
NB100-56503
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - p73 was detected in immersion fixed Hela human cell line using NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

61 Publications
APE Antibody (13B8E5C2) - BSA Free
NB100-116
Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Knockdown Validated: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1, p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ELISA

     3 Reviews

107 Publications
MBD2 Antibody
NB100-55415
Western Blot: MBD2 Antibody [NB100-55415] - Staining of Jurkat cell lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - Staining of paraffin embedded Human Skin with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, IHC, IHC-P

2 Publications
alpha Desmuslin Antibody
NBP3-13366
Western Blot: alpha Desmuslin Antibody [NBP3-13366] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Desmuslin antibody (NBP3-13366) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: alpha Desmuslin Antibody [NBP3-13366] - alpha Desmuslin antibody detects alpha Desmuslin protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Desmuslin stained by alpha Desmuslin antibody (NBP3-13366) diluted at 1:500. Blue: Fluoroshield with DAPI.

Rabbit Polyclonal
Species Mouse, Rat
Applications WB, ICC/IF, IHC