| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7208-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77021-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80051-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80050-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80045-50ug | |||
![Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Western-Blot-NBP3-16285-img0003.jpg "Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Immunofluorescence analysis of U-2 OS cells using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunocytochemistry-Immunofluorescence-NBP3-16285-img0002.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody (2Q9K10) [NBP3-16285] - Human bladder cancer using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunohistochemistry-Paraffin-NBP3-16285-img0001.jpg)
![Immunoprecipitation: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of 300ug extracts of 293T cells using 3ug FTO antibody (NBP3-16285). Western blot was performed from the immunoprecipitate using FTO antibody (NBP3-16285) at a dilition of 1:500.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunoprecipitation-NBP3-16285-img0004.jpg)
![Western Blot: FTO Antibody [NBP1-77021] - Analysis of FTO in human uterus tissue lysate with FTO antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/FTO-Antibody-Western-Blot-NBP1-77021-img0004.jpg "Western Blot: FTO Antibody [NBP1-77021] - Analysis of FTO in human uterus tissue lysate with FTO antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: FTO Antibody [NBP1-77021] - Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77021-img0003.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody [NBP1-77021] - Mouse brain tissue with FTO antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP1-77021-img0002.jpg)
![Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-27220248453212.jpg "Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: FTO Antibody (4G9) [NBP3-26565] - Image of FTO Antibody (4G9) diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-27220248573310.jpg)
![Western Blot: FTO Antibody (4G9) [NBP3-26565] - Positive Western Blot detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate, Raji whole cell lysate, Colo320 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate. All lanes: FTO Antibody at 0.7ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59, 15, 7, 13 KDa Observed band size: 59 KDa](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-282202411485835.jpg)
![Immunocytochemistry/Immunofluorescence: FTO Antibody (4G9) [NBP3-26565] - Staining of Hela cells with FTO Antibody (4G9) at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26565_rabbit-fto-mab-4g9-282202412475115.jpg)
![Western Blot: FTO Antibody [NBP2-58941] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.](http://images.novusbio.com/fullsize/FTO-Antibody-Western-Blot-NBP2-58941-img0002.jpg "Western Blot: FTO Antibody [NBP2-58941] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FTO Antibody [NBP2-58941] - Staining in human parathyroid gland and pancreas tissues using anti-FTO antibody. Corresponding FTO RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP2-58941-img0006.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody [NBP2-58941] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP2-58941-img0004.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody [NBP2-58941] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP2-58941-img0005.jpg)
![Western Blot: FTO Antibody (AG103) [NBP2-80051] - Analysis of human FTO using anti-human FTO mAb (AG103) at 1:1,000 dilution:1. hFTO (His-tagged) 100ng.2. HEK293T 100ug.3. HEK293E 100ug.4. HepG2 100ug.5. Hep3B 50ug.6. VIT1 80ug.7. THP1 74ug.8. Molt4 96ug.9. A549 48ug](http://images.novusbio.com/fullsize/FTO-Antibody-AG103-Western-Blot-NBP2-80051-img0003.jpg "Western Blot: FTO Antibody (AG103) [NBP2-80051] - Analysis of human FTO using anti-human FTO mAb (AG103) at 1:1,000 dilution:1. hFTO (His-tagged) 100ng.2. HEK293T 100ug.3. HEK293E 100ug.4. HepG2 100ug.5. Hep3B 50ug.6. VIT1 80ug.7. THP1 74ug.8. Molt4 96ug.9. A549 48ug")
![Immunohistochemistry-Paraffin: FTO Antibody (AG103) [NBP2-80051] - Immunohistochemical staining of FTO with anti-FTO (human), mAb (AG103) in brain, hypothalamus and paraventricular nucleus (5~10 ug/mL). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalinfixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain (40x).](http://images.novusbio.com/fullsize/FTO-Antibody-AG103-Immunohistochemistry-Paraffin-NBP2-80051-img0001.jpg)
![Immunoprecipitation: FTO Antibody (AG103) [NBP2-80051] - Immunoprecipitation of recombinant human FTO proteins using anti-FTO (human), mAb (AG103) at different concentrations. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb.1. hFTO (His-tagged) recombinant control 100ng.2. hFTO (His-tagged) 5ug.3. hFTO (His-tagged) 2.5ug.4. hFTO (His-tagged) 1.25ug.5. hFTO (His-tagged) 0.625ug.6. hFTO (His-tagged) 0.312ug.7. hFTO (His-tagged) 0.156ug.8. hFTO (His-tagged) 0.078ug](http://images.novusbio.com/fullsize/FTO-Antibody-AG103-Immunoprecipitation-NBP2-80051-img0002.jpg)
![Western Blot: FTO Antibody (FT62-6) [NBP2-80050] - Analysis using anti-FTO (mouse), mAb (FT62-6) at 1:2'000 dilution.A) 1: Mouse kideny (200 ug). 2: Mouse spleen (200 ug). 3: Mouse heart (200 ug). 4: Mouse liver (500 ug). 5: Mouse stomach (200 ug). 6: Mouse brain (300 ug). 7: Mouse lung (200 ug). B) 1: Mouse FTO (10 ng). 2: 3T3L1 (100 ug).](http://images.novusbio.com/fullsize/FTO-Antibody-FT62-6-Western-Blot-NBP2-80050-img0003.jpg "Western Blot: FTO Antibody (FT62-6) [NBP2-80050] - Analysis using anti-FTO (mouse), mAb (FT62-6) at 1:2'000 dilution.A) 1: Mouse kideny (200 ug). 2: Mouse spleen (200 ug). 3: Mouse heart (200 ug). 4: Mouse liver (500 ug). 5: Mouse stomach (200 ug). 6: Mouse brain (300 ug). 7: Mouse lung (200 ug). B) 1: Mouse FTO (10 ng). 2: 3T3L1 (100 ug).")
![Immunohistochemistry-Paraffin: FTO Antibody (FT62-6) [NBP2-80050] - Immunohistochemical staining of mouse FTO using anti-FTO (mouse), mAb (FT62-6) in mouse tissue (1:500 dilution, 200X). A. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse brain. A-1. Isotype control, mouse brain. B. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse uterus. B-1. Isotype control, mouse uterus.](http://images.novusbio.com/fullsize/FTO-Antibody-FT62-6-Immunohistochemistry-Paraffin-NBP2-80050-img0001.jpg)
![Immunoprecipitation: FTO Antibody (FT62-6) [NBP2-80050] - Immunoprecipitation confirmed by recombinant mouse FTO. Recombinant mouse FTO proteins at different concentrations were precipitated using anti-FTO (mouse), mAb (FT62-6). The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with rabbit anti-mouse FTO pAb. 1. mFTO (His-tagged) recombinant control 50 ng. 2. mFTO (His-tagged) 10 ug. 3. mFTO (His-tagged) 5 ug. 4. mFTO (His-tagged) 2.5 ug. 5. mFTO (His-tagged) 1.25 ug. 6. mFTO (His-tagged) 0.312 ug. 7. mFTO (His-tagged) 0.156 ug.](http://images.novusbio.com/fullsize/FTO-Antibody-FT62-6-Immunoprecipitation-NBP2-80050-img0002.jpg)
![Western Blot: FTO Antibody (FT86-4) [NBP2-80045] - Western blot analysis using anti-FTO (human), mAb (FT86-4) at 1:2'000 dilution.1: Human FTO (His-tagged) (100 ng).2: Mouse FTO (His-tagged) (100 ng).3: Rat FTO (His-tagged) (100 ng).4: HEK 293 cell lysate (100 ug).5: Human Calreticulin (His-tagged) (100 ng) (negative control).](http://images.novusbio.com/fullsize/FTO-Antibody-FT86-4-Western-Blot-NBP2-80045-img0002.jpg "Western Blot: FTO Antibody (FT86-4) [NBP2-80045] - Western blot analysis using anti-FTO (human), mAb (FT86-4) at 1:2'000 dilution.1: Human FTO (His-tagged) (100 ng).2: Mouse FTO (His-tagged) (100 ng).3: Rat FTO (His-tagged) (100 ng).4: HEK 293 cell lysate (100 ug).5: Human Calreticulin (His-tagged) (100 ng) (negative control).")
![Immunohistochemistry: FTO Antibody (FT86-4) [NBP2-80045]](http://images.novusbio.com/fullsize/FTO-Antibody-FT86-4-Immunohistochemistry-NBP2-80045-img0003.jpg)
![Immunoprecipitation: FTO Antibody (FT86-4) [NBP2-80045] - Immunoprecipitation of recombinant human FTO using anti-FTO (human), mAb (FT86-4). Recombinant human FTO proteins at different concentrations were precipitated by FT86-4. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb.](http://images.novusbio.com/fullsize/FTO-Antibody-FT86-4-Immunoprecipitation-NBP2-80045-img0001.jpg)
![Western Blot: FTO Antibody (5-2H10) [NBP2-29512] - Western blot of rat testes lysate showing specific immunolabeling of the ~ 58k Fto protein.](http://images.novusbio.com/fullsize/FTO-Antibody-5-2H10-Western-Blot-NBP2-29512-img0002.jpg "Western Blot: FTO Antibody (5-2H10) [NBP2-29512] - Western blot of rat testes lysate showing specific immunolabeling of the ~ 58k Fto protein.")
![Immunohistochemistry-Paraffin: FTO Antibody [NBP2-62659] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP2-62659-img0003.jpg "Immunohistochemistry-Paraffin: FTO Antibody [NBP2-62659] - Staining of human pancreas shows low expression as expected.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FTO Antibody [NBP2-62659] - Analysis in human parathyroid gland and pancreas tissues using Anti-FTO antibody. Corresponding FTO RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP2-62659-img0001.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody [NBP2-62659] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/FTO-Antibody-Immunohistochemistry-Paraffin-NBP2-62659-img0002.jpg)