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Dna Hypomethylation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Dna Hypomethylation Pathway and Malignant Neoplasms, Neoplasms, Carcinogenesis, Carcinoma, Cell Transformation, Neoplastic. The study of the Dna Hypomethylation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Dna Hypomethylation Pathway has been researched in relation to Methylation, Dna Methylation, Demethylation, Dna Hypermethylation, Pathogenesis. The Dna Hypomethylation Pathway complements our catalog of research reagents including antibodies and ELISA kits against DNA METHYLTRANSFERASE 3B, HISTONE H3, DNA METHYLTRANSFERASE 3A, CBS, CD70.

Top Research Reagents

We have 3377 products for the study of the Dna Hypomethylation Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB600-302
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY license.Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). <br/>Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Mouse Monoclonal
Species Human, Mouse, Bovine
Applications WB, Simple Western, ELISA

     4 Reviews

46 Publications
NB300-516
Western Blot: DNMT3B Antibody [NB300-516] - Expression level of DNMT3B by RT-PCR and Western blot (W.B.) analysis in human oral keratinocyte (HOK) and OSCC cell lines. GAPDH and a-tubulin were used as internal control, respectively. Image collected and cropped by CiteAb from the following publication (jbiomedsci.biomedcentral.com/articles/10.1186/s12929-020-00644-z), licensed under a CC-BY license.Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

     3 Reviews

18 Publications
NB100-56326
Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB100-56326] - This antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

1 Publication
NBP1-33518
Knockout Validated: CBS Antibody [NBP1-33518] - Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Knockdown Validated: CBS Antibody [NBP1-33518] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     3 Reviews

42 Publications
NBP1-86304
Immunohistochemistry-Paraffin: DPEP2 Antibody [NBP1-86304] - Staining of human spleen shows strong cytoplasmic and membranous positivity in subset of cells in red pulp and sinusoids.Immunohistochemistry: DPEP2 Antibody [NBP1-86304] - Staining of human kidney shows strong cytoplasmic positivity in tubule and glomerular cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.


Species Human
Applications WB

MAB3595
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Human Integrin aL/CD11a Monoclonal Antibody (Catalog # MAB3595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Simple Western lane view shows lysates of PBMC, Placenta, loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha L/CD11a at approximately 220 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Integrin  alpha L/CD11a Monoclonal Antibody (Catalog # MAB3595). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Mouse Monoclonal
Species Human
Applications WB, Simple Western, Flow

     1 Review

3 Publications
MAB2738
HEK293 human embryonic kidney cell line transfected with human CD27 Ligand/TNFSF7 (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human CD27 Ligand/TNFSF7 Monoclonal Antibody (Catalog # MAB2738) followed by Goat anti-mouse PE-conjugated secondary antibody (<a class=NoLineLink href=

Mouse Monoclonal
Species Human
Applications Flow

     1 Review

9 Publications
NBP2-37607
Western Blot: MTHFR Antibody (5D3) [NBP2-37607] - Western blot analysis using MTHFR mouse mAb against Rat Heart cell lysate.Immunohistochemistry-Paraffin: MTHFR Antibody (5D3) [NBP2-37607] -  Analysis of  intima cancer tissues (left) and prostate tissues (right) using MTHFR mouse mAb with DAB staining.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, IHC

292-G2
Recombinant Human IGF-II/IGF2 (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED<sub>50</sub> for this effect is 1.5‑6 ng/mL.1 μg/lane of Recombinant Human IGF‑II/IGF2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.


Species Human
Applications BA

47 Publications
NBP2-52486
Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.Immunocytochemistry/Immunofluorescence: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

NB100-56519
Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Antibody dilution of 1:10 was used. Image objective 40x.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     4 Reviews

144 Publications
NB100-791
Western Blot: MTR Antibody [NB100-791] - Cgl null mice exhibit female-specific alteration of enzymes involved in the remethylation of methionine. Western blotting analysis of hepatic BHMT, MTHFR and MTR protein expression levels in male and female WT and Cgl null mice. Values shown represent the mean+-s.d., *Pless than 0.05, **Pless than 0.01, ***Pless than 0.001. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/cgi/doi/10.1242/bio.013433) licensed under a CC-BY license.Immunohistochemistry-Paraffin: MTR Antibody [NB100-791] - Human adrenal gland. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     2 Reviews

3 Publications
NB200-111
Knockdown Validated: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT decreases p53mut stability. T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 i1/4M), Nutlin3A (5 i1/4M) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. <i>Oncotarget</i> (2014))  licensed under a CC-BY license.Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT modulates p53 stability in-vivo and synergizes with ionizing radiation to inhibit tumor growth( Sections of PSN1 xenografts treated with three consecutive doses of MK-2206 (60 mg/kg). Sections of PSN1 xenografts and in-vitro PSN1 cells fixed and stained with anti-NPM (red) and anti-p14ARF (green). Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget (2014))  licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     2 Reviews

17 Publications
NB120-13888
Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/mL.Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Ntera2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-DNMT3A (68B1446) [NB120-13888] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ChIP, Flow

     3 Reviews

124 Publications
NB100-55415
Western Blot: MBD2 Antibody [NB100-55415] - Staining of Jurkat cell lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - Staining of paraffin embedded Human Skin with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, IHC, IHC-P

2 Publications