DNMT3A Antibody (64B1446) - BSA Free

Methylation of cytosine residues is essential for mammalian development by regulating gene expression, gene silencing and genomic imprinting (1). This process is controlled by the enzyme DNA (cytosine-5)-methyltransferase 3A (DNMt3a), which is encoded by the DNMT3A gene and has a predicted molecular weight of 102 kDa. DNA methyltransferases including DNMt3a are involved in de novo cytosine methylation and maintenance of embryonic and somatic cells through the transfer of methyl groups to specific CpG structures in DNA (2). Somatic mutations in DNMT3A are responsible for cytogenetically normal acute myeloid leukemia (CN-AML). Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA are linked with cancer (3,4). Mutations in the DNMT3A gene have also been found to cause DNMT3A overgrowth syndrome and myelodysplastic syndromes.

References

1. Thakur, A., Mackin, S. J., Irwin, R. E., O'Neill, K. M., Pollin, G., & Walsh, C. (2016). Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin, 9, 53. doi:10.1186/s13072-016-0104-2

2. Ravichandran, M., Lei, R., Tang, Q., Zhao, Y., Lee, J., Ma, L., . . . Dawlaty, M. M. (2019). Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells. Cell Rep, 28(8), 1993-2003.e1995. doi:10.1016/j.celrep.2019.07.080

3. Pang, Y., Liu, J., Li, X., Xiao, G., Wang, H., Yang, G., . . . Ren, H. (2018). MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med, 22(12), 6262-6274. doi:10.1111/jcmm.13916

4. Xia, L., Huang, W., Bellani, M., Seidman, M. M., Wu, K., Fan, D., . . . Baylin, S. B. (2017). CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell, 31(5), 653-668.e657. doi:10.1016/j.ccell.2017.04.005

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 10 applications: ChIP, Chemotaxis, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IHC-WhMt, KO, WB.

Showing Publications 1 - 10 of 124. Show All 124 Publications. Collapse Publications.

Publications using NB120-13888	Applications	Species
Nguyet-Minh Hoang, Yunxia Liu, Paul D. Bates, Alexa R. Heaton, Angelica F. Lopez, Peng Liu, Fen Zhu, Ruoyu Chen, Apoorv Kondapelli, Xiyu Zhang, Paul E. Selberg, Vu N. Ngo, Melissa C. Skala, Christian M. Capitini, Lixin Rui Targeting DNMT3A-mediated oxidative phosphorylation to overcome ibrutinib resistance in mantle cell lymphoma Cell Reports Medicine 2024-03-29 [PMID: 38554704]
Lisa-Marie Brenner, Florian Meyer, Haiqian Yang, Anja R Köhler, Pavel Bashtrykov, Ming Guo, Albert Jeltsch, Cristiana Lungu, Monilola A Olayioye Repeat DNA methylation is modulated by adherens junction signaling. Communications biology 2024-03-11 [PMID: 38454140]
Yang C, Deng L, Bao F et al. Sevoflurane with Low Concentration Decrease DNA Methylation on Chronic Obstructive Pulmonary Disease (COPD)-Related Gene Promoter in COPD Rat COPD 2023-12-01 [PMID: 38010369] (WB)	WB
Ren G, Li H, Hong D et al. LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter BMC cancer 2023-09-23 [PMID: 37742010] (WB, Human)	WB	Human
Fernandes C, da Silva R, Wood P et al. Titanium-Enriched Medium Promotes Environment-Induced Epigenetic Machinery Changes in Human Endothelial Cells Journal of Functional Biomaterials 2023-02-27 [PMID: 36976055]
da C. Fernandes C, da Silva R, de Almeida G et al. Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt-Chromium Journal of Functional Biomaterials 2023-02-26 [PMID: 36976051] (WB, Human)	WB	Human
Narabayashi H, Koma C, Nakata K et al. Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions Frontiers in molecular biosciences 2022-10-21 [PMID: 36339704] (ChIP, Mouse)	ChIP	Mouse
Ni Q, Sun J, Wang Y et al. mTORC1 is required for epigenetic silencing during beta-cell functional maturation Molecular metabolism 2022-08-05 [PMID: 35940555] (WB, IF/IHC, Mouse) Details: IHC- 1:100 dilution used (supplemental Fig. 1), WB - 1:1000 dilution used	WB, IF/IHC	Mouse
Richard Albert J Application of an allele-specific pipeline to study DNA methylation inheritance and dynamics in the early embryo Thesis 2020-01-01 (ICC/IF, Mouse)	ICC/IF	Mouse
Lu F, Yi Y, Vladimirova O et al. Epigenetic Landscape of HIV Infection in Primary Human Macrophage bioRxiv 2022-01-27 [PMID: 35319230]
Liu X, Cui H The palliative effects of folic acid on retinal microvessels in diabetic retinopathy via regulating the metabolism of DNA methylation and hydroxymethylation Bioengineered 2021-12-01 [PMID: 34874218] (Chemotaxis, Rat)	Chemotaxis	Rat
Aronson BE, Scourzic L, Shah V Et al. A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus Developmental cell 2021-10-26 [PMID: 34710357]
Zhao J, Lin H, Huang K. Mesenchymal Stem Cell-derived Extracellular Vesicles Transmitting MicroRNA-34a-5p Suppress Tumorigenesis of Colorectal Cancer Through c-MYC/DNMT3a/PTEN Axis Molecular neurobiology 2021-10-08 [PMID: 34623601] (WB, Human)	WB	Human
da C Fernandes CJ, da Silva RA, Fretes Wood P Et al. The molecular pathway triggered by zirconia in endothelial cells involves epigenetic control Tissue & cell 2021-08-18 [PMID: 34425516] (WB, Human)	WB	Human
Kim KP, Li C, Bunina D et al. Donor cell memory confers a metastable state of directly converted cells Cell stem cell 2021-04-07 [PMID: 33848472]
Huang Z, Yu J, Cui W, et al. The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins Science Advances 2021-01-01 [PMID: 33523915] (WB, Mouse)	WB	Mouse
Broche J, Kungulovski G, Bashtrykov P et al. Genome-wide investigation of the dynamic changes of epigenome modifications after global DNA methylation editing Nucleic acids research 2020-12-09 [PMID: 33300025] (WB)	WB
Cardenas H, Fang F, Jiang G et al. Methylomic Signatures of High Grade Serous Ovarian Cancer Epigenetics 2020-12-08 [PMID: 33289590]
Zaro BW, Noh JJ, Mascetti VL et al. Proteomic analysis of young and old mouse hematopoietic stem cells and their progenitors reveals post-transcriptional regulation in stem cells J Control Release 2020-11-21 [PMID: 33236985]
Wei J, Cheng J, Waddell NJ et al. DNA Methyltransferase 3A Is Involved in the Sustained Effects of Chronic Stress on Synaptic Functions and Behaviors Molecules 2020-11-19 [PMID: 33230530] (WB, Human)	WB	Human
Jia Y, Xie H, Zhang J, Ying H Induction of TGF-beta receptor I expression in a DNA methylation-independent manner mediated by DNMT3A downregulation is involved in early-onset severe preeclampsia FASEB J. 2020-08-14 [PMID: 32794622] (WB)	WB
Magno Guimaraes D, deLucas da Silva Almeida F, Moraes Castilho R DNA methyltransferase expression is associated with cell proliferation in salivary mucoepidermoid carcinoma J. Oral Pathol. Med. 2020-08-02 [PMID: 32740989]
Yagi M, Kabata M, Tanaka A et al. Identification of distinct loci for de novo DNA methylation by DNMT3A and DNMT3B during mammalian development Nat Commun 2020-06-24 [PMID: 32581223] (WB, KO, Mouse)	WB, KO	Mouse
Ginno PA, Gaidatzis D, Feldmann A et al. A genome-scale map of DNA methylation turnover identifies site-specific dependencies of DNMT and TET activity Nat Commun 2020-05-29 [PMID: 32471981] (WB, Mouse)	WB	Mouse
Espinosa-Diez C, Wilson R, Mukherjee R et al. DNA damage dependent hypomethylation regulates the pro-angiogenic LncRNA MEG9 bioRxiv 2018-10-14 (WB, Human)	WB	Human
McAninch D, Makela JA, La HM et al. SOX3 promotes generation of committed spermatogonia in postnatal mouse testes Sci Rep 2020-04-21 [PMID: 32317665] (IHC-WhMt, Mouse)	IHC-WhMt	Mouse
Lavery L A, Ure K et al. Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome. Elife 2020-11-03 [PMID: 32159514] (Mouse)		Mouse
Ravichandran M, Lei R, Tang Q et al. Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells Cell Rep 2019-08-20 [PMID: 31433977] (WB, Mouse)	WB	Mouse
Heyn P, Logan CV, Fluteau A et al. Gain-of-function DNMT3A mutations cause microcephalic dwarfism and hypermethylation of Polycomb-regulated regions. Nat. Genet. 2018-11-26 [PMID: 30478443] (WB, Mouse)	WB	Mouse
van Mierlo G, Dirks RAM, De Clerck L et al. Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency. Cell Stem Cell 2018-11-14 [PMID: 30472157] (Human)		Human
Cyphert HA, Walker EM, Hang Y et al. Examining How the MAFB Transcription Factor Affects Islet b Cell Function Postnatally. Diabetes. 2018-11-13 [PMID: 30425060] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Pang Y, Liu J, Li X et al. MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer.. J. Cell. Mol. Med. 2018-10-16 [PMID: 30324719] (ICC/IF, Human)	ICC/IF	Human
Chatterjee B, Lin MH, Chen CC et al. DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells. Biochim Biophys Acta Gene Regul Mech. 2018-11-01 [PMID: 30300721] (WB, Human)	WB	Human
La HM, Makela JA, Chan AL, et al Identification of dynamic undifferentiated cell states within the male germline. Nat Commun. 2018 Jul 19 [PMID: 30026551] (ICC/IF, IHC-Fr, Mouse)	ICC/IF, IHC-Fr	Mouse
La HM, Makela JA, Chan AL, et al Identification of dynamic undifferentiated cell states within the male germline. Nat Commun. 2018 Jul 19 [PMID: 30026551] (ICC/IF, IHC-Fr, Mouse)	ICC/IF, IHC-Fr	Mouse
Masaki Yagi, Satoshi Kishigami, Akito Tanaka, Katsunori Semi, Eiji Mizutani, Sayaka Wakayama, Teruhiko Wakayama, Takuya Yamamoto, Yasuhiro Yamada Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation. Nature 2017-12-14 [PMID: 28746308]
Sheikh MA, Malik YS, Zhu X. RA-Induced Transcriptional Silencing of Checkpoint Kinase-2 through Promoter Methylation by Dnmt3b Is Required for Neuronal Differentiation of P19 Cells J. Mol. Biol. 2017-07-13 [PMID: 28712951] (Mouse)		Mouse
Ni Q, Gu Y, Xie Y et al. Raptor regulates functional maturation of murine beta cells. Nat Commun. 2017-06-09 [PMID: 28598424] (WB, Mouse)	WB	Mouse
Xia Limin, Huang Wenjie, Bellani Marina et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell 2017-09-06 [PMID: 28486105] (WB, ICC/IF, Chemotaxis, Human)	WB, ICC/IF, Chemotaxis	Human
Mahadevan S, Sathappan V, Utama B et al. Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming. Sci Rep 2017-03-20 [PMID: 28317850] (ICC/IF, Mouse)	ICC/IF	Mouse
Neri F, Rapelli S, Krepelova A et al. Intragenic DNA methylation prevents spurious transcription initiation. Nature. 2017-02-22 [PMID: 28225755] (WB, Human)	WB	Human
Acharya MM, Baddour AA, Kawashita T et al. Epigenetic determinants of space radiation-induced cognitive dysfunction. Sci Rep. 2017-02-21 [PMID: 28220892] (ICC/IF, Mouse)	ICC/IF	Mouse
Thakur A, Mackin SJ, Irwin RE et al. Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin. 2016-11-29 [PMID: 27895716] (WB, Mouse)	WB	Mouse
Hoang TV, Horowitz ER, Chaffee BR et al. Lens development requires DNMT1 but takes place normally in the absence of both DNMT3A and DNMT3B activity. Epigenetics. 2016-11-08 [PMID: 27824296] (Mouse)		Mouse
Daniel FI, Alves SR, Vieira DS et al. Immunohistochemical expression of DNA methyltransferases 1, 3a, and 3b in actinic cheilitis and lip squamous cell carcinomas. J. Oral Pathol. Med. 2016-05-09 [PMID: 27159259] (IHC-P, Human) Details: DNMT1 antibody (clone 60B1220.1; Imgenex IMG-261A) was used at 1:1500 dilution for IHC-P analysis of tissues from human subjects with actinic cheilitis and lip squamous cell carcinoma (LSCC), and non-neoplastic tissue (Figure 1).	IHC-P	Human
Xu X, Tan X, Hulshoff MS et al. Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells. FEBS Lett. 2016-04-01 [PMID: 27012941] (WB, Human)	WB	Human
Hong B, Su Z, Zhang C et al. Reserpine Inhibit the JB6 P+ Cell Transformation Through Epigenetic Reactivation of Nrf2-Mediated Anti-oxidative Stress Pathway. AAPS J. 2016-03-17 [PMID: 26988984] (WB, Mouse)	WB	Mouse
Dhawan S, Dirice E, Kulkarni RN, Bhushan A. Inhibition of TGF-beta signaling promotes human pancreatic beta cell replication. Diabetes. 2016-03-02 [PMID: 26936960] (WB, Human)	WB	Human
Amouroux R, Nashun B, Shirane K et al. De novo DNA methylation drives 5hmC accumulation in mouse zygotes. Nat. Cell Biol. 2016-02-01 [PMID: 26751286] (ICC/IF, Mouse)	ICC/IF	Mouse
Li Y, Seah MK, O'Neill C. Mapping global changes in nuclear cytosine base modifications in the early mouse embryo. Reproduction. 2016-02-01 [PMID: 26660107] (IHC-WhMt, Mouse) Details: DNMT3B Antibody (clone 52A1018; Imgenex IMG-184A) was used for immunofluorescence staining of the murine whole embryos which were fixed at in 4% formaldehyde for 30?minutes at room temperature and permeabilized in 0.5% Tween20 & Triton X100. The antibody was used at 1:150 dilution (see full text for detailed protocol).	IHC-WhMt	Mouse
Kita H, Okamoto K et al. Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas. Oncol Lett 2015-01-08 [PMID: 26622550] (WB, Human)	WB	Human
Tsukada Y, Akiyama T, Nakayama KI. Maternal TET3 is dispensable for embryonic development but is required for neonatal growth. Sci Rep. 2015-10-28 [PMID: 26507142] (ICC/IF, IHC-WhMt, Mouse) Details: DNMT3a antibody was used for ICC/IF and IHC-whole mount staining of fully grown oocytes at the germinal vesicle stage and zygotes produced by in vitro fertilization in experiments involving Tet3 conditional knockout mice. The IHC protocol involved 20?minutes of fixation at RT in 4% PFA-PBS with 0.2% Triton X-100, 1 hour blocking with 1% BSA -0.05% Tween 20, ON incubation with DNMT3a antibody at 1:1000 dilution, detection using AF568-conjugated secondary (see full text for detailed protocol).	ICC/IF, IHC-WhMt	Mouse
Dhawan S, Tschen SI, Zeng C et al. DNA methylation directs functional maturation of pancreatic B cells J. Clin. Invest. 2015-07-01 [PMID: 26098213] (IHC-P, Mouse)	IHC-P	Mouse
Robaina MC, Mazzoccoli L, Arruda VO et al. Deregulation of DNMT1, DNMT3B and miR-29s in Burkitt lymphoma suggests novel contribution for disease pathogenesis Exp. Mol. Pathol. 2015-03-04 [PMID: 25746661] (IHC-P, Human) Details: DNMT3B antibody (clone 52A1018) was used at 1:200 dilution for IHC-P application on tissue microarray /TMA slides of Burkitt lymphoma tissues with placental tissue included as positive control (Fig. 1).	IHC-P	Human
Neri F, Incarnato D, Krepelova A et al. Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics Cell Rep. 2015-02-04 [PMID: 25660018] (WB, Mouse) Details: DNMT3B antibody (clone 52A1018) used for WB analysis of lysates from mouse embryonic stem cell / mESCs which were transfected with either control shRNA (shCtr) or shRNAs targeting Dnmts namely Sh-Dnmt1, Sh-Dnmt3a, Sh-Dnmt3b and Sh-Dnmt3a/b (Figure 4E).	WB	Mouse
Guo Y, Shu L, Zhang C et al. Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1 Biochem. Pharmacol. 2015-01-29 [PMID: 25640947] (WB, Human) Details: DNMT3A antibody used for WB on lysates of HT29 human colon cancer cells treated or not with 2.5uM or 5uM Curcumin, and 2.5uM azadeoxycytidine/5AZA + 100uM trichostatin A/TSA. Curcumin was found to downregualte the expression levels of DNMT3A protein in a concentration-dependent manner (Fig 5).	WB	Human
Huichalaf Claudia, Micheloni Stefano, Ferri Giulia et al. DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level. PLoS One. 2014-01-01 [PMID: 25545674] (Chemotaxis, Human)	Chemotaxis	Human
Wang L, Zhang C, Guo Y et al. Blocking of JB6 Cell Transformation by Tanshinone IIA: Epigenetic Reactivation of Nrf2 Antioxidative Stress Pathway. AAPS J. 2014-11-01 [PMID: 25274607] (WB, Mouse) Details: DNMT3a antibody used for WB on lysates of JB6 P+ cells (a mouse keratinocyte cell line) treated or not with Tanshinone IIA (TIIA), an active component in Salvia miltiorrhiza that has been reported to activate Nrf2 pathway.	WB	Mouse
Emperle M, Rajavelu A, Reinhardt R et al. Cooperative DNA binding and protein/DNA fiber formation increases the activity of the Dnmt3a DNA methyltransferase J. Biol. Chem. 2014-08-21 [PMID: 25147181] (WB)	WB
Guimaraes DM, Antunes DM, Duarte CM et al. DNA methyltransferase immunohistochemical expression in odontogenic tumours J. Oral Pathol. Med. 2014-07-24 [PMID: 25060239] (IHC-P, Human) Details: Dnmt3a antibody used for IHC-P at 1:200 dilution on formalin-fixed and paraffin-embedded tissue sections of human ameloblastomas, calcifying cystic odontogenic tumours, calcifying epithelial tumours, adenomatoid odontogenic tumours, keratocystic odontogenic tumours, ameloblastic fibromas, ameloblastic fibro-odontomas, central odontogenic fibromas, peripheral odontogenic fibromas and odontogenic myxomas.	IHC-P	Human
Paredes-Gonzalez X, Fuentes F, Su ZY, Kong AN. Apigenin Reactivates Nrf2 Anti-oxidative Stress Signaling in Mouse Skin Epidermal JB6 P + Cells Through Epigenetics Modifications. AAPS J. 2014-05-16 [PMID: 24830944] (WB, Mouse) Details: JB6 P+ mouse skin epidermal cells, Fig 6a.	WB	Mouse
Chuang C-H, Wang W-J, Li C-F et al. The combination of the prodrugs perforin-CEBPD and perforin-granzyme B efficiently enhances the activation of caspase signaling and kills prostate cancer. Cell Death Dis. 2014-01-01 [PMID: 24810056] (WB)	WB
Hara S, Takano T, Ogata M et al. Establishment of a Conditional Transgenic System Using the 2A Peptide in the Female Mouse Germline. J. Reprod. Dev. 2014-03-15 [PMID: 24632680] (WB, Mouse) Details: DNMT3A2-transgenic mouse models amd DNMT3A2-transfected cells (Figs 1C, 2E, F). DNMT3A2 was detected at approx. 85 kDa (Fig 1C), DNMT3A1 was detected at approx. 135kDa (Fig 2E). The specificity of the DNMT3A mAb was validated in DNMT3A2 transgenic mouse m	WB	Mouse
Serra FW, Fang M, Park SM et al. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. Elife 2014-01-01 [PMID: 24623306] (Chemotaxis, Human)	Chemotaxis	Human
Hara S, Takano T, Fujikawa T et al. Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting. Hum. Mol. Genet. 2014-03-18 [PMID: 24599402] (WB, Mouse) Details: Mouse WT oocytes and oocytes DNMT3A2 transgenic mice, Figs 1, 2D-E, 4A. The mAb detected both 3A (130 kDa) and the smaller 3A2 (85 kDa) isoforms. DNMT3A2 is a short DNMT3A isoform produced from an alternative promoter. Note: The specificity of the antibod	WB	Mouse
Hara ES1, Ono M1, Eguchi T2 et al. miRNA-720 Controls Stem Cell Phenotype, Proliferation and Differentiation of Human Dental Pulp Cells. PLoS One 2013-12-30 [PMID: 24386225] (ICC/IF, Human)	ICC/IF	Human
Wongtrakoongate P, Li J, Andrews PW. DNMT3B inhibits the re-expression of genes associated with induced pluripotency. Exp Cell Res 2013-12-10 [PMID: 24333507] (WB, Human)	WB	Human
Zhou JX, Dhawan S, Fu H et al. Combined modulation of polycomb and trithorax genes rejuvenates beta cell replication. J Clin Invest 2013-10-08 [PMID: 24216481] (WB, Mouse)	WB	Mouse
Obata Y, Wakai T, Hara S, Kono T et al. Long exposure to mature ooplasm can alter DNA methylation at imprinted loci in non-growing oocytes but not in prospermatogonia. Reproduction. 2014-01-01 [PMID: 24123131] (ICC/IF, Mouse) Details: Oocyte/prospermatogonium fusions, Fig 3.	ICC/IF	Mouse
Takahashi Mayumi, Kamei Yasutomi, Ehara Tatsuya et al. Analysis of DNA methylation change induced by Dnmt3b in mouse hepatocytes. Biochem Biophys Res Commun. 2013-05-17 [PMID: 23611774] (Mouse)		Mouse
Park ES, Park YK, Shin CY et al. Hepatitis B virus inhibits liver regeneration via epigenetic regulation of urokinase-type plasminogen activator. Hepatology. 2013-08-01 [PMID: 23483589]
Leitch Harry G, McEwen Kirsten R, Turp Aleksandra et al. Naive pluripotency is associated with global DNA hypomethylation. Nat Struct Mol Biol. 2013-03-01 [PMID: 23416945]
Sheikh MA, Malik YS, Yu H et al. Epigenetic regulation of Dpp6 expression by Dnmt3b and its novel role in the inhibition of RA induced neuronal differentiation of P19 cells. PLoS One. 2013-01-01 [PMID: 23409053]
Zimmermann N, Zschocke J, Perisic T et al. Antidepressants inhibit DNA methyltransferase 1 through reducing G9a levels. Biochem J. 2012-11-15 [PMID: 22880885]
Hong GM, Bain LJ. Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1. Toxicol Appl Pharmacol. 2012-05-01 [PMID: 22426358]
Stadtfeld M, Apostolou E, Ferrari F et al. Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nat Genet. 2012-03-04 [PMID: 22387999]
Selvakumar T, Gjidoda A, Hovde SL, Henry RW. Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases. J Biol Chem. 2012-03-02 [PMID: 22219193]
Kadriu B, Guidotti A, Chen Y, Grayson DR. DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain. J Comp Neurol. 2012-06-15 [PMID: 22134929] (IHC-Fr, WB, Mouse) Details: Antibodies cited: 1. DNMT1 (IMG-261A): WB (mouse brain cortical extracts), Table 1; IHC (frozen) (mouse brain), Figs 5, 6, 8B, 8C. 2. DNMT3A (IMG-268A): WB (mouse brain cortical extracts), Table 1; IHC (frozen) (mouse brain), Fig 7. Note: The specificitie	IHC-Fr, WB	Mouse
Yang Jian, Wei Xiaowei, Wu Qingxiang et al. Clinical significance of the expression of DNA methyltransferase proteins in gastric cancer. Mol Med Rep. 2011-11-01 [PMID: 21887466] (IF/IHC, Human)	IF/IHC	Human
Kurdikova Vendula, Smolinsky Radovan, Gvozdik Lumir. Mothers matter too: benefits of temperature oviposition preferences in newts. PLoS One. 2011-01-01 [PMID: 21887330] (WB)	WB
Pawlak M, Jaenisch R. De novo DNA methylation by Dnmt3a and Dnmt3b is dispensable for nuclear reprogramming of somatic cells to a pluripotent state. Genes Dev. 2011-05-15 [PMID: 21576263]
Nasonkin Igor O, Lazo Kevin, Hambright Dustin et al. Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina. J Comp Neurol. 2011-07-01 [PMID: 21452232]
Lahousse S A, Hoenerhoff M, Collins J et al. Gene expression and mutation assessment provide clues of genetic and epigenetic mechanisms in liver tumors of oxazepam-exposed mice. Vet Pathol. 2011-07-01 [PMID: 21147764] (IF/IHC, Mouse)	IF/IHC	Mouse
Daniel Filipe Ivan, Rivero Elena Riet Correa, Modolo Filipe et al. Immunohistochemical expression of DNA methyltransferases 1, 3a and 3b in oral leukoplakias and squamous cell carcinomas. Arch Oral Biol. 2010-12-01 [PMID: 20951977] (Human)		Human
Kashiwagi K, Nimura K, Ura K, Kaneda Y. DNA methyltransferase 3b preferentially associates with condensed chromatin. Nucleic Acids Res. 2011-02-01 [PMID: 20923784]
Meilinger D, Fellinger K, Bultmann S et al. Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells. EMBO Rep. 2009-11-01 [PMID: 19798101]
May A, Kirchner R, Muller H et al. Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres. Biol Reprod. 2009-01-01 [PMID: 18784354] (ICC/IF, Mouse) Details: IF/ICC (mouse blastomeres), Fig. 5A.	ICC/IF	Mouse
Kubo N, Uehara R, Uemura S et al. Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells bioRxiv 2023-09-08 (ICC/IF, Mouse)	ICC/IF	Mouse
Molognoni Fernanda, Cruz Adriana T, Meliso Fabiana M et al. Epigenetic reprogramming as a key contributor to melanocyte malignant transformation. Epigenetics. 2011-04-01 [PMID: 21343701] (WB, Mouse)	WB	Mouse
Lambrot R, Kimmins S. Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines. Int J Androl. 2011-04-01 [PMID: 20497257] (WB, Human)	WB	Human
Pierard Valerie, Guiguen Allan, Colin Laurence et al. DNA cytosine methylation in the bovine leukemia virus promoter is associated with latency in a lymphoma-derived B-cell line: potential involvement of direct inhibition of cAMP-responsive element (CRE)-binding protein/CRE modulator/activation transcription factor binding. J Biol Chem. 2010-06-18 [PMID: 20413592] (WB)	WB
Wu Y, Alvarez M, Slamon DJ et al. Caspase 8 and maspin are downregulated in breast cancer cells due to CpG site promoter methylation. BMC Cancer. 2010-02-04 [PMID: 20132554] Details: Dnmt1 (IMG-261A),Dnmt2 (IMG-281), Dnmt3a (IMG-268A), Dnmt3b (IMG-184A). WB: Human breast cancer MCF-7, MB231, BT474 cell lines treated with or without 5-aza-dc, Fig 4.
Fan H, Liu D, Qiu X et al. A functional polymorphism in the DNA methyltransferase-3A promoter modifies the susceptibility in gastric cancer but not in esophageal carcinoma. BMC Med. 2010-02-03 [PMID: 20128888]
Ko CY, Hsu HC, Shen MR et al. Epigenetic silencing of CCAAT/enhancer-binding protein delta activity by YY1/polycomb group/DNA methyltransferase complex. J Biol Chem. 2008-11-07 [PMID: 18753137] (WB) Details: HeLa cells in Fig. 7A (IF), 7B (WB following a DNA Affinity Precipitation Assay of nuclear extracts), 7C and D (ChIP): 1. IMG-261A (Dnmt1) 2. IMG-268A (Dnmt3a) 3. IMG-184A (Dnmt3b).	WB
Zhu Feng, Xia Xiaojun, Liu Bigang et al. IKKalpha shields 14-3-3sigma, a G(2)/M cell cycle checkpoint gene, from hypermethylation, preventing its silencing. Mol Cell. 2007-07-20 [PMID: 17643371] (Chemotaxis)	Chemotaxis
Lucifero D, La Salle S, Bourc'his D et al. Coordinate regulation of DNA methyltransferase expression during oogenesis. BMC Dev Biol. 2007-04-19 [PMID: 17445268] (WB, Mouse) Details: Products cited for WB (growing mouse oocytes and type B spermatogonia): 1. DNMT3a (IMG-268A), Fig 5a, b (middle panel). The antibody detected both the DNMT3a (908 aa) and DNMT3a2 (689 aa) isoforms. 2. DNMT3b (IMG-184A), Fig 5c (top panel). The antibody de	WB	Mouse
Yu V, Yong F, Chen K et al. Establishment of beta cell heterogeneity via differential CpG methylation atNnat bioRxiv 2023-02-05 (IHC, Mouse)	IHC	Mouse
Hirasawa R, Chiba H, Kaneda M et al. Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. Genes Dev. 2008-06-15 [PMID: 18559477] (ICC/IF, Mouse) Details: Antibodies cited: 1. Dnmt3a (IMG-268a): IF/ICC (mouse oocytes and embryos), Fig. 1a. 2. Dnmt3b (IMG-184): IF/ICC (mouse occytes and embryos), Fig. 1c.	ICC/IF	Mouse
Xia G, Schneider-Stock R, Diestel A et al. Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation. Biochem Biophys Res Commun. 2008-05-02 [PMID: 18302936] (WB, Human) Details: (human NCI N87 gastric carcinoma cells): WB (Fig 1) 1. Dnmt1 (IMG-261). 2. Dnmt3a (IMG-268) 3. Dnmt3b (IMG-184).	WB	Human
Linhart HG, Lin H, Yamada Y et al. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev 2007-12-01 [PMID: 18056424] (WB, Mouse)	WB	Mouse
Oh Bong-Kyeong, Kim Haeryoung, Park Hye-Jung et al. DNA methyltransferase expression and DNA methylation in human hepatocellular carcinoma and their clinicopathological correlation. Int J Mol Med. 2007-07-01 [PMID: 17549390] (Human)		Human
Park IY, Sohn BH, Yu E et al. Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology. 2007-04-01 [PMID: 17408664] (WB) Details: (Chang liver and Hep G2 cell lines): 1. IMG-261(DNMT1): WB, Figs. 1A, 4A,B. 2. IMG-268A (DNMT3A): WB, ChIP, Figs. 1B, 2B, 4A,B.	WB
Nimura K, Ishida C, Koriyama H et al. Dnmt3a2 targets endogenous Dnmt3L to ES cell chromatin and induces regional DNA methylation. Genes Cells. 2006-10-01 [PMID: 16999741]
Shen Y, Chow J, Wang Z, Fan G. Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cells. Hum Mol Genet. 2006-09-01 [PMID: 16870691] (ICC/IF)	ICC/IF
Iwatani M, Ikegami K, Kremenska Y et al. Dimethyl sulfoxide has an impact on epigenetic profile in mouse embryoid body. Stem Cells. 2006-11-01 [PMID: 16840553]
Tsumura A, Hayakawa T, Kumaki Y et al. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells. 2006-07-01 [PMID: 16824199]
La Salle S, Trasler JM. Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. Dev Biol 2006-08-01 [PMID: 16725135] (WB, Mouse)	WB	Mouse
Jones B, Chen J. Inhibition of IFN-gamma transcription by site-specific methylation during T helper cell development. EMBO J. 2006-06-07 [PMID: 16724115] (Chemotaxis)	Chemotaxis
Ueda Y, Okano M, Williams C et al. Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome. Development. 2006-03-01 [PMID: 16501171]
Galetzka D, Tralau T, Stein R, Haaf T. Expression of DNMT3A transcripts and nucleolar localization of DNMT3A protein in human testicular and fibroblast cells suggest a role for de novo DNA methylation in nucleolar inactivation. J Cell Biochem. 2006-07-01 [PMID: 16453278]
MacDonald JL, Gin CS, Roskams AJ. Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development. Dev Biol. 2005-12-15 [PMID: 16288735] (IHC-P)	IHC-P
Qiu YY, Mirkin BL, Dwivedi RS. Inhibition of DNA methyltransferase reverses cisplatin induced drug resistance in murine neuroblastoma cells. Cancer Detect Prev. 2005-01-01 [PMID: 16185816]
Park Hye-Jung, Yu Eunsil, Shim Yhong-Hee. DNA methyltransferase expression and DNA hypermethylation in human hepatocellular carcinoma. Cancer Lett. 2006-02-28 [PMID: 15885882] (Human)		Human
Oka M, Meacham AM, Hamazaki T et al. De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine. Oncogene. 2005-04-28 [PMID: 15735669]
Stempak JM, Sohn KJ, Chiang EP et al. Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model. Carcinogenesis. 2005-05-01 [PMID: 15695236]
Feng J, Chang H, Li E, Fan G. Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system. J Neurosci Res. 2005-03-15 [PMID: 15672446]
Leu YW, Yan PS, Fan M et al. Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer. Cancer Res. 2004-11-15 [PMID: 15548683] (Chemotaxis)	Chemotaxis
Park IY, Sohn BH, Choo JH et al. Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development. J Cell Biochem. 2005-02-15 [PMID: 15543560]
Weisenberger DJ, Velicescu M, Cheng JC et al. Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation. Mol Cancer Res. 2004-01-01 [PMID: 14757847] (WB) Details: Products cited for WB [wild-type mouse embryonic stem (ES) cells and ES knockouts of Dnmt3a, Dnmt3b, and 3a/b]: 1. DNMT3a (IMG-268A), Fig 2A, B. The specificity of the antibody is knockout mouse validated as it did not detect Dnmt3a in either the 3a-/- (M	WB
Yan Q, Cho E, Lockett S, Muegge K. Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin. Mol Cell Biol. 2003-12-01 [PMID: 14612388]
Chen T, Ueda Y, Dodge JE et al. Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol Cell Biol. 2003-08-01 [PMID: 12897133] (WB, Mouse) Details: WB [mouse embryonic stem (ES) cell lines and ES cells expressing various Dnmt1, 3a and 3b isoforms], Figs 1B and 7A.	WB	Mouse
Choi MS, Shim YH, Hwa JY et al. Expression of DNA methyltransferases in multistep hepatocarcinogenesis. Hum Pathol. 2003-01-01 [PMID: 12605361]
Chen T, Ueda Y, Xie S, Li E. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem. 2002-10-11 [PMID: 12138111]
Dennis K, Fan T, Geiman T et al. Lsh, a member of the SNF2 family, is required for genome-wide methylation. Genes Dev. 2001-11-15 [PMID: 11711429]
Show All 124 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	01/30/2019	View Summary Application Western Blot Sample Tested cell Species Human Lot D-5
Enlarge	4 reviewed by: Verified Customer	WB	Human	01/23/2017	View Summary Application Western Blot Sample Tested recombinant purified protein Species Human Lot AB1106138-C3 Comments Comments 1ug of recombinant DNMT3A protein. 4-12% Bis tris gel
Enlarge	4 reviewed by: Nathan Burns	WB	Other	08/02/2016	View Summary Application Western Blot Species Other

Array NB120-13888

• DNMT3A Antibodies
• DNMT3A ELISA Kits
• DNMT3A Kit
• DNMT3A Lysate
• DNMT3A Proteins