Nucleolin Antibody

Nucleolin (NCL, also called Protein C23) is a major phosphoprotein present in nucleolus of growing eukaryotic cells which is implicated in the synthesis as well as maturation of ribosomes. Localized mainly to nucleolus, NCL may also be found in cytoplasmic mRNP granules containing untranslated mRNAs (mRNP granule complex contains - NCL, ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs). NCL interacts with APTX, NSUN2, TERT, ERBB4, GZF1, GZF1, NVL and C1QBP, and is a part of SWAP complex (NPM1, NCL, PARP1 and SWAP70) as well as another complex containing NCL, HTATSF1/Tat-SF1, CDK9 and CCNT1, RNA polymerase II, SUPT5H. NCL may be found associated with intranucleolar chromatin and pre-ribosomal particles where it induces chromatin de-condensation via binding to histone H1 and play a key role in pre-rRNA transcription as well as ribosome assembly. NCL has also been suggested to implicate in the process of transcriptional elongation and it binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Besides being essential to rRNA processing in nucleolus, NCL has several other functions such as transcription regulation, modulation of mRNA stability and DNA replication and recombination.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Plant.

We have publications tested in 7 applications: Chemotaxis, ICC/IF, IF/IHC, IHC-Fr, IM, Simple Western, WB.

Showing Publications 1 - 10 of 26. Show All 26 Publications. Collapse Publications.

Publications using NB600-241	Applications	Species
Freya Molenberghs, Marlies Verschuuren, Lauran Vandeweyer, Sarah Peeters, Johannes J Bogers, Claudina Perez Novo, Wim Vanden Berghe, Hans De Reu, Nathalie Cools, Mario Schelhaas, Winnok H De Vos Lamin B1 curtails early human papillomavirus infection by safeguarding nuclear compartmentalization and autophagic capacity. Cellular and molecular life sciences : CMLS 2024-03-18 [PMID: 38485766]
Godet AC, Roussel E, David F et al. Long non-coding RNA Neat1 and paraspeckle components are translational regulators in hypoxia eLife 2022-12-22 [PMID: 36546462] (Simple Western, Mouse) Details: Dilution used in Simple Western 1:50	Simple Western	Mouse
Hoffmann F, Umbreit C, Kruger T et al. Identification of Proteomic Markers in Head and Neck Cancer Using MALDI-MS Imaging, LC-MS/MS, and Immunohistochemistry. Prot. Clin. Appl. 2018-11-26 [PMID: 30411850] (IHC-Fr, Human)	IHC-Fr	Human
Banerjee S, Aponte-Diaz D, Yeager C et al. Hijacking of Multiple Phospholipid Biosynthetic Pathways and Induction of Membrane Biogenesis by a Picornaviral 3CD Protein bioRxiv 2017-10-29 [PMID: 29782554] (ICC/IF, Human)	ICC/IF	Human
Molenberghs F, Verschuuren M, Vandeweyer L et al. Lamin B1 curtails early human papillomavirus infection by safeguarding nuclear compartmentalization and autophagic capacity Research Square 2022-08-05 (WB, Human)	WB	Human
Zhang K, Miorin L, Makio T et al. Nsp1 protein of SARS-CoV-2 disrupts the mRNA export machinery to inhibit host gene expression Science advances 2021-02-01 [PMID: 33547084]
Wu R, Li L, Bai Y et al. The long noncoding RNA LUCAT1 promotes colorectal cancer cell proliferation by antagonizing Nucleolin to regulate MYC expression Cell Death Dis 2020-10-23 [PMID: 33097685] (Chemotaxis)	Chemotaxis
Podratz JL, Tang JJ, Polzin MJ et al. Mechano growth factor interacts with nucleolin to protect against cisplatin-induced neurotoxicity Exp. Neurol. 2020-06-05 [PMID: 32511954] (ICC/IF, Mouse)	ICC/IF	Mouse
Okur MN, Lee JH, Osmani W et al. Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation Nucleic Acids Res. 2020-01-23 [PMID: 31970402] (Chemotaxis, Human)	Chemotaxis	Human
Park OH, Park J, Yu M et al. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay. Genes Dev. 2016-09-15 [PMID: 27798850] (WB, Human)	WB	Human
Corne TD, Sieprath T, Vandenbussche J et al. Uncoordinated regulation of focal adhesions and cytoskeletal tension due to the loss of a-type lamins. Cell Adh Migr 2016-10-28 [PMID: 27791462] (WB, Human)	WB	Human
Robijns J, Molenberghs F, Sieprath T et al. In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells. Sci Rep. 2016-07-27 [PMID: 27461848] (WB)	WB
Leaderer D, Cashman SM, Kumar-Singh R. Topical application of a G-Quartet aptamer targeting nucleolin attenuates choroidal neovascularization in a model of age-related macular degeneration. Exp. Eye Res. 2015-09-12 [PMID: 26368850] (IF/IHC, Mouse)	IF/IHC	Mouse
Sieprath T, Corne TD, Nooteboom M et al. Sustained accumulation of prelamin A and depletion of lamin A/C both cause oxidative stress and mitochondrial dysfunction but induce different cell fates. Nucleus 2015-05-21 [PMID: 25996284] (WB)	WB
Vermezovic J, Adamowicz M, Santarpia L et al. Notch is a direct negative regulator of the DNA-damage response Nat. Struct. Mol. Biol. 2015-04-20 [PMID: 25895060] (WB, Human)	WB	Human
Litchfield LM, Riggs KA, Hockenberry AM, Oliver LD, Barnhart KG et al. (2012) Identification and Characterization of Nucleolin as a COUP-TFII Coactivator of Retinoic Acid Receptor ? Transcription in Breast Cancer Cells. PLoS ONE 7(5): e38278. [PMID: 22693611] (ICC/IF, Human)	ICC/IF	Human
Stepinski D. Nucleolin level in plant root meristematic cells under chilling stress and recovery Micron 2012-03-12 [PMID: 22483616] (IM, Plant)	IM	Plant
K Kume, Y Iizumi, M Shimada et al. Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway. Genes Cells; 15 (4): 339-349. 2010-01-01 [PMID: 20298436] (WB, Rat)	WB	Rat
Drygin D, Siddiqui-Jain A, O'Brien S et al. Anticancer Activity of CX-3543: A Direct Inhibitor of rRNA Biogenesis. Cancer Res;69(19):7653-7661. 2009-01-01 [PMID: 19738048]
Gonzalez V, Guo K, Hurley L et al. Identification Characterization of Nucleolin as a c-myc G-quadruplex-binding Protein. J Biol Chem;284(35):23622-23635. 2009-01-01 [PMID: 19581307] (Chemotaxis, Human)	Chemotaxis	Human
Levitt ES, Clark MJ, Jenkins PM et al. Differential effect of membrane cholesterol removal on mu- and delta-opioid receptors: a parallel comparison of acute and chronic signaling to adenylyl cyclase. J Biol Chem 2009-08-14 [PMID: 19520863]
Fatemi SH, Folsom TD, Reutiman TJ, Sidwell RW. Viral regulation of aquaporin 4, connexin 43, microcephalin and nucleolin. Schizophr Res 2008-01-01 [PMID: 17997079]
Novoselov, SV et al. Selenoprotein H is a nucleolar thioredoxin-like protein with a unique expression pattern. J Biol Chem;282(16):11960-8. 2007-04-20 [PMID: 17337453] (ICC/IF, Mouse)	ICC/IF	Mouse
Jin, J et al. Identification of novel proteins associated with both alpha-synuclein and DJ-1. Mol Cell Proteomics;6(5):845-59. 2007-05-01 [PMID: 16854843] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Huddelson, JP et al. Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin. J Biol Chem;281(22):15121-8. 2006-06-02 [PMID: 16571724] (Chemotaxis, Human, Mouse)	Chemotaxis	Human, Mouse
Hanakahi, LA et al. Nucleolin is one component of the B-cell-specific transcription factor switch region binding protein, LR1. PNAS 94: 3605-3610. 1997-01-01 [PMID: 9108024] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Show All 26 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	ICC	Human	02/09/2017	View Summary Application Immunocytochemistry Sample Tested Human prostate cancer cells Species Human Lot C-3
Enlarge	5 reviewed by: Verified Customer	IF		08/22/2012	View Summary Application Immunofluorescence

Array NB600-241

• Nucleolin Antibodies
• Nucleolin Proteins