Fibrillarin Antibody (38F3)

Fibrillarin / Nop1p, was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 6 confirmed species: Human, Mouse, C. elegans, Drosophila, Plant, S. pombe.

We have publications tested in 8 applications: IB, ICC/IF, IF/IHC, IHC-Fr, IHC-P, IHC-WhMt, IP, WB.

Showing Publications 1 - 10 of 24. Show All 24 Publications. Collapse Publications.

Publications using NB300-269	Applications	Species
Miranda L. Wilson, Shannon N. Romano, Nitya Khatri, Devora Aharon, Yulong Liu, Odelya H. Kaufman, Bruce W. Draper, Florence L. Marlow Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component Mios Nature Communications 2024-06-19 [PMID: 38898112]
Qinyu Hao, Minxue Liu, Swapna Vidhur Daulatabad, Saba Gaffari, You Jin Song, Rajneesh Srivastava, Shivang Bhaskar, Anurupa Moitra, Hazel Mangan, Elizabeth Tseng, Rachel B Gilmore, Susan M Frier, Xin Chen, Chengliang Wang, Sui Huang, Stormy Chamberlain, Hong Jin, Jonas Korlach, Brian McStay, Saurabh Sinha, Sarath Chandra Janga, Supriya G Prasanth, Kannanganattu V Prasanth, Jeannie T Lee, Kevin Struhl Monoallelically expressed noncoding RNAs form nucleolar territories on NOR-containing chromosomes and regulate rRNA expression eLife 2024-01-19 [PMID: 38240312]
Klump BM, Perez GI, Patrick EM et al. TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assembly Cell reports 2023-06-01 [PMID: 37267110]
Papandreou M, Konstantinidis G, Tavernarakis N Nucleophagy delays aging and preserves germline immortality Nature Aging 2022-12-23 [PMID: 37118512] (IP, IB, Mouse)	IP, IB	Mouse
Palikaras K, Mari M, Ploumi C et al. Age-dependent nuclear lipid droplet deposition is a cellular hallmark of aging in Caenorhabditis elegans Aging cell 2023-01-31 [PMID: 36718841] (WB, C. elegans)	WB	C. elegans
Hibino E, Ichiyama Y, Tsukamura A et al. Bex1 is essential for ciliogenesis and harbours biomolecular condensate-forming capacity BMC biology 2022-02-10 [PMID: 35144600] (ICC/IF)	ICC/IF
Wu T, Nance J, Chu F, Fazzio T Characterization of R-loop-interacting proteins in embryonic stem cells reveals roles in ribosomal RNA processing and gene expression Molecular & Cellular Proteomics 2021-08-01 [PMID: 34478875]
Procida T, Friedrich T, Jack APM, et al. JAZF1, A Novel p400/TIP60/NuA4 Complex Member, Regulates H2A.Z Acetylation at Regulatory Regions International journal of molecular sciences 2021-01-12 [PMID: 33445503] (ICC/IF, Human)	ICC/IF	Human
Sural S, Liang CY, Wang FY et al. HSB-1/HSF-1 pathway modulates histone H4 in mitochondria to control mtDNA transcription and longevity Sci Adv 2020-10-01 [PMID: 33087356]
Begik O, Lucas MC, Liu H et al. Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures Genome Biol. 2020-05-07 [PMID: 32375858] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Hsiung K, Liu K, Tsai T et al. Defects in CISD-1, a mitochondrial iron-sulfur protein, lower glucose level and ATP production in Caenorhabditis elegans Biomedical Journal 2020-02-01 [PMID: 32200954] (WB, C. elegans)	WB	C. elegans
Chen P, Chen Y, Chu T et al. Nucleolar control by a non apoptotic p53 caspases deubiquitinylase axis promotes resistance to bacterial infection FASEB J 2020-01-10 [PMID: 31914708]
Wong IN, Neo JP, Oehler J et al. The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast Elife 2019-12-19 [PMID: 31855181] (WB, S. pombe)	WB	S. pombe
Tiku V, Kew C, Mehrotra P et al. Nucleolar fibrillarin is an evolutionarily conserved regulator of bacterial pathogen resistance. Nat Commun 2018-09-06 [PMID: 30190478] (WB, C. elegans)	WB	C. elegans
Buchwalter A, Hetzer MW. Nucleolar expansion and elevated protein translation in premature aging Nat Commun 2017-08-30 [PMID: 28855503] (ICC/IF, Human)	ICC/IF	Human
Tiku V, Jain C, Raz Y et al. Small nucleoli are a cellular hallmark of longevity Nat Commun 2016-08-30 [PMID: 28853436] (WB, C. elegans)	WB	C. elegans
Robaszkiewicz E, Idziak-Helmcke D, Tkacz MA et al. The arrangement of Brachypodium distachyon chromosomes in interphase nuclei. J. Exp. Bot. 2016-10-01 [PMID: 27588463] (IF/IHC, Plant)	IF/IHC	Plant
Mitrea DM, Cika JA, Guy CS et al. Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA Elife 2016-03-01 [PMID: 26836305] (ICC/IF, Mouse)	ICC/IF	Mouse
Itatani Y, Sonoshita M, Kakizaki F et al. Characterization of Aes nuclear foci in colorectal cancer cells. J. Biochem. 2015-07-29 [PMID: 26229111] (ICC/IF, Human) Details: Fibrillarin antibody was used for ICC/IF analysis of HCT116 cells overexpressing AcGFP-tagged Aes (AcGFP-Aes) and TLE1 (Fig. 1B).	ICC/IF	Human
Zaballos MA, Cantero W, Azpiazu N. The TALE Transcription Factor Homothorax Functions to Assemble Heterochromatin during Drosophila Embryogenesis. PLoS ONE. 2015-03-21 [PMID: 25794008] (IF/IHC, Drosophila)	IF/IHC	Drosophila
Lee CC, Tsai YT, Kao CW et al. Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans. Cell Death Dis 2014-04-11 [PMID: 24722283] (IHC-WhMt, C. elegans)	IHC-WhMt	C. elegans
Zillner K, Filarsky M, Rachow K et al. Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 Nucleic Acids Res 2013-04-10 [PMID: 23580549] (ICC/IF, Human)	ICC/IF	Human
Su H, Xu T, Ganapathy S et al. Elevated snoRNA biogenesis is essential in breast cancer Oncogene 2013-04-01 [PMID: 23542174] (IHC-P, WB, Human)	IHC-P, WB	Human
Beyret E, Lin H. Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse Dev Biol 2011-07-15 [PMID: 21539824] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Show All 24 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IF	Mouse	06/14/2021	View Summary Application Immunofluorescence Sample Tested fibroblasts,Mouse embryonic fibroblasts Species Mouse Lot 9A-1147725
	5 reviewed by: Varnesh Tiku	WB	C. elegans	01/09/2019	View Summary Application Western Blot Sample Tested 4% PFA-PBS fixed frozen sections from murine brain,Tissue Lysates Species C. elegans Lot 38F3
Enlarge	5 reviewed by: Bryan Tinsley	ICC	Human	06/09/2014	View Summary Application Immunocytochemistry Species Human
	5 reviewed by: Verified Customer	WB	Other	04/24/2014	View Summary Application Western Blot Sample Tested fibroblasts Species Other Lot 92412

Array NB300-269

• Fibrillarin Antibodies
• Fibrillarin Lysate
• Fibrillarin Proteins