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Melan-A/MART-1 Antibody (A19-P)

Images

 
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Confocal microscopy image of human fetal inner ear tisue section stained with Melan-A antibody (green), 1:200 dilution, incubated over night. ...read more
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Human tissue (4 um section) stained with anti - Melan A monospecific clonal antibody.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications ICC/IF, IHC
Clone
A19-P
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Melan-A/MART-1 Antibody (A19-P) Summary

Description
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Peptide derived from C-terminal sequence of human Melan-A/MART-1. Antibody recognizes the epitope between Glu105 - Pro115.
Epitope
Glu105 - Pro115
Specificity
This antibody is specific for the C terminal portions of Melan-A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MLANA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
Reviewed Applications
Read 4 Reviews rated 4.8
using
NBP1-30151 in the following applications:

Publications
Read Publications using
NBP1-30151 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26547111). Use in Canine reported in secitific publication PMID: 32423311

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for Melan-A/MART-1 Antibody (A19-P)

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • Melan-A
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Background

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melan-A/MART-1 Antibody (NBP1-30151)(22)

We have publications tested in 4 confirmed species: Human, Mouse, Canine, Monkey.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(2)
IF/IHC
(6)
IHC-Fr
(1)
IHC-P
(8)
All Applications
Filter By Species
Human
(7)
Mouse
(7)
Canine
(1)
Monkey
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NBP1-30151 Applications Species
Aoki H, Tomita H, Hara A, Kunisada T Postnatal expression of Kit ligand affects pigmentation of the epidermis The Journal of investigative dermatology 2023-07-21 [PMID: 37482288]
Kruse B, Buzzai AC, Shridhar N et al. CD4+ T cell-induced inflammatory cell death controls immune-evasive tumours Nature 2023-06-01 [PMID: 37316667] (IHC-P, Human)

Details:
IHC-P: 1:500
IHC-P Human
Dietsch, B;Weller, C;Sticht, C;de la Torre, C;Kramer, M;Goerdt, S;G�raud, C;Wohlfeil, SA; Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern BMC cancer 2023-05-13 [PMID: 37179302] (ICC/IF, Mouse) ICC/IF Mouse
Estrada J, Zhan J, Mitchell P et al. OncoVEXmGM-CSFexpands tumor antigen-specific CD8+ T-cell response in preclinical models Journal for immunotherapy of cancer 2023-05-01 [PMID: 37164449] (IHC-P, Mouse) IHC-P Mouse
Eichhoff OM, Stoffel CI, K�sler J et al. ROS Induction Targets Persister Cancer Cells with Low Metabolic Activity in NRAS-Mutated Melanoma Cancer research 2023-03-22 [PMID: 36946761]
Hosoya M, Kitama T, Iwabu K et al. Development of the stria vascularis in the common marmoset, a primate model Scientific reports 2022-11-17 [PMID: 36396805] (IHC-Fr, Monkey)

Details:
Dilution used in IHC-Fr 1:500
IHC-Fr Monkey
Lee JH, Ahn Y, Lee HE et al. Wearable Surface-Lighting Micro-Light-Emitting Diode Patch for Melanogenesis Inhibition Advanced healthcare materials 2022-10-03 [PMID: 36189834] (IF/IHC) IF/IHC
Eshiba S, Namiki T, Mohri Y Et al. Stem cell spreading dynamics intrinsically differentiate acral melanomas from nevi Cell reports 2021-08-03 [PMID: 34348144]
van Beelen ESA, van der Valk WH, de Groot JCMJ et al. Migration and fate of vestibular melanocytes during the development of the human inner ear Dev Neurobiol 2020-10-19 [PMID: 33075185] (IF/IHC, Human) IF/IHC Human
Nakano S, Abe Y, Nakajima K et al. Establishment of a mouse model for post-inflammatory hyperpigmentation Pigment Cell Melanoma Res 2020-07-04 [PMID: 32623834] (IF/IHC) IF/IHC
Show All 22 Publications.

Reviews for Melan-A/MART-1 Antibody (NBP1-30151) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-30151:
Filter by Applications
IHC-P
(2)
ICC
(2)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Melan-A/MART-1 NBP1-30151
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 10/30/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman fetal inner ear
SpeciesHuman
LotDB050-01-06B
Immunocytochemistry Melan-A/MART-1 NBP1-30151
Enlarge
4
reviewed by:
Verified Customer
ICC Mouse 09/02/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse melanocyte cell line
SpeciesMouse
Immunocytochemistry Melan-A/MART-1 NBP1-30151
Enlarge
5
reviewed by:
Verified Customer
ICC Human 09/02/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman hair follicle culture
SpeciesHuman
Immunohistochemistry-Paraffin Melan-A/MART-1 NBP1-30151
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 09/02/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman adult facial skin
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Melan-A/MART-1 Antibody (NBP1-30151) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Verified Customer
10/30/2019
Application: IHC-P
Species: Human

Verified Customer
09/02/2013
Application: ICC
Species: Mouse

Verified Customer
09/02/2013
Application: ICC
Species: Human

Bioinformatics

Gene Symbol MLANA
Entrez
Uniprot