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ID3 Antibody (OTI8B3)

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Western Blot: ID3 Antibody (8B3) [NBP2-02136] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug ...read more
Immunocytochemistry/ Immunofluorescence: ID3 Antibody (OTI8B3) [NBP2-02136] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3.
Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human thyroid tissue using anti-ID3 mouse monoclonal antibody.
Flow Cytometry: ID3 Antibody (OTI8B3) [NBP2-02136] - Analysis of Jurkat cells, using anti-ID3 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human colon tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human Kidney tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human liver tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human Ovary tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (8B3) [NBP2-02136] - Staining of paraffin-embedded Human prostate tissue using anti-ID3 mouse monoclonal antibody.
Flow Cytometry: ID3 Antibody (8B3) [NBP2-02136] - Analysis of Hela cells, using anti-ID3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI8B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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ID3 Antibody (OTI8B3) Summary

Immunogen
Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ID3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
Theoretical MW
12.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ID3 Antibody (OTI8B3)

  • BHLHB25
  • bHLHb251R21
  • Class B basic helix-loop-helix protein 25
  • DNA-binding protein inhibitor ID-3
  • HEIR1
  • HEIR-1
  • Helix-loop-helix protein HEIR-1
  • ID-like protein inhibitor HLH 1R21
  • Inhibitor of DNA binding 3
  • inhibitor of DNA binding 3, dominant negative helix-loop-helix protein

Background

Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ID3 Antibody (NBP2-02136). (Showing 1 - 1 of 1 FAQ).

  1. Can this monoclonal antibody (NBP2-02136) be applicated for mouse IHC?
    • Our antibody NBP2-02136 has not yet been tested for use in mouse samples. However, the immunogen for this product does share 96% homology to the mouse sequence, so there is a good chance that this antibody can recognize the mouse protein.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ID3
Entrez