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![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Detection of NPC3 in rat small intestine membrane preparations (20 ug). (1) 2 ug/ml and (2) 4 ug/ml. ECL: 30 minute exposure.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NB400-128-img0004.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Detection of NPC3 in rat small intestine membrane preparations (20 ug). (1) 2 ug/ml and (2) 4 ug/ml. ECL: 30 minute exposure.")
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Staining of human small intestine, enterocytes.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NB400-128-img0005.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Decrease in the biliary cholesterol secretion in L1-Tg mice. Immunoblot detection of hepatic NPC1L1 protein in L1-Tg mice using an anti-NPC1L1 (Niemann-Pick type C1 Like-1) antibody. WT, wild-type. Image collected and cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/s12944-019-1179-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NB400-128-img0006.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody - BSA Free [NB400-128] - Hepatic NPC1L1‐mediated steatosis in the liver of L1‐Tg mice. A, Hepatic expression of the human NPC1L1 protein in L1‐Tg mice demonstrated by immunoblotting using an anti‐NPC1L1 antibody. alpha ‐Tubulin, a loading control. B, Decrease of biliary cholesterol levels in L1‐Tg mice. Data are expressed as the mean ± SEM n = 6 (WT) & 7 (L1‐Tg). C, Photographic images of the livers of WT & L1‐Tg mice fed a control fat diet (CFD) or a high‐fat diet (HFD) for 2 weeks. The coin diameter was 1 cm. D, Hematoxylin & eosin (H&E) & Oil Red O staining of the livers of WT & L1‐Tg mice fed a HFD for 2 weeks. E & F, Hepatic cholesterol levels (E, left), hepatic triglyceride (TG) levels (E, right), body weight (BW) (F, left), & the ratios of liver weight to BW (L/B ratio) (F, right) in each group of mice fed a CFD or HFD for 2 weeks. Data are expressed as the mean ± SEM n = 11 (WT‐CFD) & 7 (the other groups). Statistical analyses for significant differences were performed using Bartlett's test, followed by a parametric Tukey–Kramer multiple‐comparison test (E, right; F) or a non‐parametric Steel–Dwass test (E, left) (##P < 0.01 vs CFD controls; **P < 0.01 vs. the other groups; *P < 0.05 among two groups; NS, not significantly different among groups) as well as a two‐sided t test (††P < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32123832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-128_rabbit-polyclonal-niemann-pick-type-c1-like-1-antibody-310202416165636.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - Detection of NPC1L1 protein in lysate of human small intestine tissue using Niemann-Pick type C1 Like-1 antibody at 2ug/ml concentration.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NB400-127-img0007.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - Detection of NPC1L1 protein in lysate of human small intestine tissue using Niemann-Pick type C1 Like-1 antibody at 2ug/ml concentration.")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - HepG2 cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody (NB400-127) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-127-img0008.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - Analysis of a FFPE tissue section of mouse small intestine using 1:200 dilution of NPC1L1 antibody (NB400-127). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NB400-127-img0009.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-89182-img0003.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-89182-img0002.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of NPC1L1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NPC1L1 antibody generated moderate staining of hepatocyte cytoplasm.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NBP2-89182-img0001.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-86732] - Host: Rabbit. Target Name: NPCL1. Sample Type: Fetal Liver lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-86732-img0001.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-86732] - Host: Rabbit. Target Name: NPCL1. Sample Type: Fetal Liver lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: Niemann-Pick type C1 Like-1 Overexpression Lysate (Adult Normal) [NBP2-10341] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Niemann-Pick type C1 Like-1.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10341-img0002.jpg "Western Blot: Niemann-Pick type C1 Like-1 Overexpression Lysate (Adult Normal) [NBP2-10341] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Niemann-Pick type C1 Like-1.")
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-94542] - Analysis of extracts of various cell lines, using Niemann-Pick type C1 Like-1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-94542-img0001.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-94542] - Analysis of extracts of various cell lines, using Niemann-Pick type C1 Like-1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.")
![Immunohistochemistry: Niemann-Pick type C1 Like-1 Antibody (4B10) [NBP3-15211] - IHC image of Niemann-Pick type C1 Like-1 antibody diluted at 1:100 and staining in paraffin-embedded human liver tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-4B10-Immunohistochemistry-NBP3-15211-img0001.jpg "Immunohistochemistry: Niemann-Pick type C1 Like-1 Antibody (4B10) [NBP3-15211] - IHC image of Niemann-Pick type C1 Like-1 antibody diluted at 1:100 and staining in paraffin-embedded human liver tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Niemann-Pick type C1 Like-1 Antibody (SR1169) [NBP3-22027] - Western blot analysis using NBP3-22027 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22027_rabbit-niemann-pick-type-c1-like-1-mab-sr1169-5720231531219.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody (SR1169) [NBP3-22027] - Western blot analysis using NBP3-22027 in HeLa cell lysate.")