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![Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of GPX4 on Zebrafish tissue lysates using anti-GPX4 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Western-Blot-NBP2-75511-img0005.jpg "Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of GPX4 on Zebrafish tissue lysates using anti-GPX4 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of MCF-7 cells labeling GPX4 with Rabbit anti-Gluthathione Peroxidase/GPX4 antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GPX4 antibody. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunocytochemistry-Immunofluorescence-NBP2-75511-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Glutathione Peroxidase 4/GPX4 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunohistochemistry-Paraffin-NBP2-75511-img0008.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded human kidney tissue using anti-GPX4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunohistochemistry-Paraffin-NBP2-75511-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded mouse testis tissue using anti-GPX4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunohistochemistry-Paraffin-NBP2-75511-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded rat epididymis tissue using anti-GPX4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunohistochemistry-Paraffin-NBP2-75511-img0003.jpg)
![Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody at 1/500 dilution.Lane 1: Hela-si NT cell lysateLane 2: Hela-si GPX4 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 22 kDaObserved band size: 20,22 kDaExposure time: 1 minutes 40 seconds;4-20% SDS-PAGE gel. was shown to specifically react with GPX4 in Hela-si NT cells. Weakened band was observed when Hela-si GPX4 sample was tested. Hela-si NT and Hela-si GPX4 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Rabbit anti-GAPDH , 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75511_rabbit-monoclonal-glutathione-peroxidase-4-gpx4-antibody-ju11-31-western-blot-187202483435..jpg)
![Western Blot: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Western blot analysis of GPX4 on rat testis tissue lysate using anti- GPX4 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Western-Blot-NBP2-76933-img0001.jpg "Western Blot: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Western blot analysis of GPX4 on rat testis tissue lysate using anti- GPX4 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - ICC staining GPX4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76933-img0004.jpg)
![Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- GPX4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunohistochemistry-NBP2-76933-img0005.jpg)
![Flow Cytometry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - Flow cytometric analysis of HepG2 cells with GPX4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Flow-Cytometry-NBP2-76933-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - ICC staining GPX4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76933-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-76933] - ICC staining GPX4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76933-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-54979] - Staining in human testis and pancreas tissues using anti-GPX4 antibody. Corresponding GPX4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunohistochemistry-Paraffin-NBP2-54979-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-54979] - Staining in human testis and pancreas tissues using anti-GPX4 antibody. Corresponding GPX4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-54979] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunohistochemistry-Paraffin-NBP2-54979-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-54979] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunohistochemistry-Paraffin-NBP2-54979-img0002.jpg)
![Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-54979] - Cyst(e)ine promotes Glutathione Peroxidase 4/GPX4 protein synthesis partly through Rag-mTORC1-4EBP signaling. l) Representative images from immunohistochemical staining of kidney tumor tissue microarray for Glutathione Peroxidase 4/GPX4 and P-4EBP1. m) Summary and statistical analysis of immunohistochemical staining results for Glutathione Peroxidase 4/GPX4 and P-4EBP1 in tumor tissue microarray. Tissue microarray analysis revealed that P-4EBP1 staining correlated with Glutathione Peroxidase 4/GPX4 level. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33707434/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-54979_rabbit-polyclonal-glutathione-peroxidase-4-gpx4-antibody-20920231412570.jpg)
![Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-54979] - Cyst(e)ine promotes GPX4 protein synthesis partly through Rag-mTORC1-4EBP signaling. i WB analysis of protein expression in control (sgCon) & 4EBP1/2-double-knockout (DKO-1 & DKO-2) UMRC6 cells. Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/33707434), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54979_rabbit-polyclonal-glutathione-peroxidase-4-gpx4-antibody-310202415171336.jpg)
![Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-54979] - mTORC1 inhibition sensitizes cancer cells or tumors to ferroptosis.a UMRC6 cells were treated with 1 μM RSL3 (or 1 μM ML162), or 1 μM Torin1, or both combined with or without 5 μM Ferrostatin-1 (Ferr-1) or 100 μM deferoxamine (DFO) for 6 h. Then lipid peroxidation was assessed using BODIPY™ 581/591 C11 staining followed by FACS analysis. b Bar graph showing the viability of UMRC6 cells treated with drugs using indicated combination. Concentration of each drug is described as follows. RSL3, 1 μM; ML162, 1 μM; Torin1, 1 μM; Ferr-1, 5 μM; DFO, 100 μM. n = 3. c Bar graph showing the viability of indicated cells treated with 300 nM RSL3, or 1 μM Torin1, or both for 8 h. n = 5. d Bar graph showing the viability of indicated cells treated with 300 nM ML162, or 1 μM Torin1, or both for 8 h. n = 5. e Bar graph showing the viability of indicated cells treated with different concentrations of cystine for 48 h. n = 4. f Plot showing the viability of indicated cells treated with different concentrations of erastin for 36 h. n = 4. g Volumes of PDX tumors in mice treated daily with 30 mg/kg IKE, or 10 mg/kg AZD8055, or both at different time points as shown. n = 5 mice per group. h Percentages of P-4EBP1-, GPX4-, or 4-HNE-positive stained cells per field. n = 5 randomly selected high-power fields per group. i Haematoxylin & eosin & immunohistochemical staining of PDX tumor samples collected from mice at the end of treatments described in g. For all panels, error bars are mean ± SD. If not otherwise specified, n indicates biologically independent repeats. P value was determined by two-tailed unpaired Student’s t test. For f & g, p value was determined by two-way ANOVA test. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33707434), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54979_rabbit-polyclonal-glutathione-peroxidase-4-gpx4-antibody-31020241613639.jpg)
![Western Blot: Glutathione Peroxidase 4 (Isoform a and c) Antibody [NB100-41379] - Analysis of Glutathione Peroxidase 4 (Isoform a and c) in Human Testis lysate using this antibody at 0.1ug/ml.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-Isoform-a-and-c-Antibody-Western-Blot-NB100-41379-img0007.jpg "Western Blot: Glutathione Peroxidase 4 (Isoform a and c) Antibody [NB100-41379] - Analysis of Glutathione Peroxidase 4 (Isoform a and c) in Human Testis lysate using this antibody at 0.1ug/ml.")
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4 (Isoform a and c) Antibody [NB100-41379] - IHC analysis of Glutathione Peroxidase 4 (Isoform a and c) in paraffin embedded Human Testis using this antibody at 0.3 ug/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-Isoform-a-and-c-Antibody-Immunohistochemistry-Paraffin-NB100-41379-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-56139] - Staining in human testis and pancreas tissues using anti-GPX4 antibody. Corresponding GPX4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunohistochemistry-Paraffin-NBP2-56139-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-56139] - Staining in human testis and pancreas tissues using anti-GPX4 antibody. Corresponding GPX4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-56139] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56139-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-56139] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunohistochemistry-Paraffin-NBP2-56139-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP2-56139] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunohistochemistry-Paraffin-NBP2-56139-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) [NBP3-07344] - Confocal immunofluorescence image of HepG2 cells stained with Glutathione Peroxidase 4/GPX4 Mouse Monoclonal Antibody (LHM 2) followed by goat anti- Mouse CF488 (green). RedDot is used to label the nuclei red.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-LHM-2-Immunocytochemistry-Immunofluorescence-NBP3-07344-img0001.jpg "Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) [NBP3-07344] - Confocal immunofluorescence image of HepG2 cells stained with Glutathione Peroxidase 4/GPX4 Mouse Monoclonal Antibody (LHM 2) followed by goat anti- Mouse CF488 (green). RedDot is used to label the nuclei red.")
![Western Blot: Glutathione Peroxidase 4/GPX4 Antibody [NBP1-54691] - 25 ug of indicated human cell extracts were loaded onto a 10% - 20% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended dilution for NBP1-54691 is 1 - 3 ug/mL. Recognizes 19 kDa cytoplasmic form in these cell lines, mitochondrial form of 22 kDa appears absent in these samples.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Western-Blot-NBP1-54691-img0004.jpg "Western Blot: Glutathione Peroxidase 4/GPX4 Antibody [NBP1-54691] - 25 ug of indicated human cell extracts were loaded onto a 10% - 20% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended dilution for NBP1-54691 is 1 - 3 ug/mL. Recognizes 19 kDa cytoplasmic form in these cell lines, mitochondrial form of 22 kDa appears absent in these samples.")
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP1-54691] - Human Heart Tissue, antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-Immunohistochemistry-Paraffin-NBP1-54691-img0003.jpg)
![Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9) [NBP3-15362] - Western blot analysis of extracts of various cell lines, using Glutathione Peroxidase 4/GPX4 Rabbit mAb (NBP3-15362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-7O4Q9-Western-Blot-NBP3-15362-img0002.jpg "Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9) [NBP3-15362] - Western blot analysis of extracts of various cell lines, using Glutathione Peroxidase 4/GPX4 Rabbit mAb (NBP3-15362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9) [NBP3-15362] - Western blot analysis of extracts from normal (control) and Glutathione Peroxidase 4/GPX4 knockout U-87MG cell pools, using Glutathione Peroxidase 4/GPX4 antibody (NBP3-15362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-7O4Q9-Knockout-Validated-NBP3-15362-img0001.jpg)
![Western Blot: Glutathione Peroxidase 4 Overexpression Lysate (Adult Normal) [NBP2-10796] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glutathione Peroxidase 4.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10796-img0001.jpg "Western Blot: Glutathione Peroxidase 4 Overexpression Lysate (Adult Normal) [NBP2-10796] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glutathione Peroxidase 4.")
![Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody (9D4) [NBP3-26545] - Image of Glutathione Peroxidase 4/GPX4 Antibody (9D4) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26545_rabbit-glutathione-peroxidase-4-gpx4-mab-9d4-26220241422523.jpg "Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody (9D4) [NBP3-26545] - Image of Glutathione Peroxidase 4/GPX4 Antibody (9D4) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![ELISA: Human Glutathione Peroxidase 4/GPX4 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31795] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31795_human-glutathione-peroxidase-4-gpx4-ready-to-use-elisa-kit-206202415351031.png "ELISA: Human Glutathione Peroxidase 4/GPX4 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31795] - Standard Curve Reference")