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![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding WASL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding WASL RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Western-Blot-NBP1-82512-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP1-82512] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82512-img0010.jpg)
![Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Western-Blot-NBP1-82512-img0008.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human cerebral cortex shows moderate to strong positivity in neuronal cells.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0011.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human kidney shows moderate to strong positivity in cells in tubules and glomeruli.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0012.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human skeletal muscle shows weak positivity in myocytes.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0013.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human stomach shows moderate to strong positivity in glandular cells.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0014.jpg)
![Simple Western: N-WASP Antibody [NBP1-82512] - Simple Western lane view shows a specific band for N-WASP in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Simple-Western-NBP1-82512-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: N-WASP Antibody [NBP1-82512] - Generation of N-WASP knockdown cell lines. A-549 and SK-MES-1 cells were treated with N-WASP siRNA/non-targeting siRNA (NT) at 0.5 ug/ml and then analysed for N-WASP expression after 48 h. A QPCR analysis of N-WASP transcript expression demonstrates a significant decrease in expression in siRNA treated cells, n = 4 replicates. b) PCR also demonstrates a decrease in N-WASP expression. d) Representative image showing knockdown of N-WASP at protein level in siRNA treated cells at 48 h. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3219-3) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Knockdown-Validated-NBP1-82512-img0016.jpg)
![Western Blot: N-WASP Antibody [NBP1-82512] - Generation of nWASP knockdown cell lines. A-549 & SK-MES-1 cells were treated with nWASP siRNA/non-targeting siRNA (NT) at 0.5 μg/ml & then analysed for nWASP expression after 48 h. a QPCR analysis of nWASP transcript expression demonstrates a significant decrease in expression in siRNA treated cells, n = 4 replicates. b PCR also demonstrates a decrease in nWASP expression. c Quantitative analysis of Western blots (n = 4) shows significant decrease in nWASP protein expression in both A-549 & SK-MES1 cell lines after 48 h siRNA treatment. d Representative image showing knockdown of nWASP at protein level in siRNA treated cells at 48 h Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28351346), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82512_rabbit-polyclonal-n-wasp-antibody-310202415392573.jpg)
![Western Blot: N-WASP Antibody [NBP1-82512] - nWASP activity affects A-549 & SK-MES-1 cell growth. a, b nWASP inhibition using 10 μM wiskostatin treatment significantly impairs the growth of A-549 & SK-MES-1 cells, respectively. c, d A significant effect on growth is also evident after 3 days in nWASP knockdown A-549 & SK-MES-1 cells, respectively, when compared to non-targeting control treated cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28351346), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82512_rabbit-polyclonal-n-wasp-antibody-310202415304216.jpg)
![Western Blot: N-WASP Antibody (4P5M9) [NBP3-16103] - Western blot analysis of extracts of HeLa cells, using N-WASP/N-WASP Rabbit mAb (NBP3-16103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/N-WASP-Antibody-4P5M9-Western-Blot-NBP3-16103-img0003.jpg "Western Blot: N-WASP Antibody (4P5M9) [NBP3-16103] - Western blot analysis of extracts of HeLa cells, using N-WASP/N-WASP Rabbit mAb (NBP3-16103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: N-WASP Antibody (4P5M9) [NBP3-16103] - Immunohistochemistry of paraffin-embedded mouse kidney using N-WASP/N-WASP Rabbit mAb (NBP3-16103) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/N-WASP-Antibody-4P5M9-Immunohistochemistry-Paraffin-NBP3-16103-img0002.jpg)
![Western Blot: N-WASP Antibody (4P5M9) [NBP3-16103] - Western blot analysis of extracts of various cell lines, using N-WASP/N-WASP Rabbit mAb (NBP3-16103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/N-WASP-Antibody-4P5M9-Western-Blot-NBP3-16103-img0004.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody (4P5M9) [NBP3-16103] - Immunohistochemistry of paraffin-embedded human placenta using N-WASP/N-WASP Rabbit mAb (NBP3-16103) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/N-WASP-Antibody-4P5M9-Immunohistochemistry-Paraffin-NBP3-16103-img0001.jpg)
![Western Blot: N-WASP Antibody (5F4) [H00008976-M04] - Analysis of WASL expression in IMR-32 (Cat # L008V1).](http://images.novusbio.com/fullsize/N-WASP-Antibody-5F4-Western-Blot-H00008976-M04-img0007.jpg "Western Blot: N-WASP Antibody (5F4) [H00008976-M04] - Analysis of WASL expression in IMR-32 (Cat # L008V1).")
![Immunocytochemistry/Immunofluorescence: N-WASP Antibody (5F4) [H00008976-M04] - Analysis of monoclonal antibody to WASL on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/N-WASP-Antibody-5F4-Immunocytochemistry-Immunofluorescence-H00008976-M04-img0005.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody (5F4) [H00008976-M04] - Analysis of monoclonal antibody to WASL on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 0.8 ug/ml](http://images.novusbio.com/fullsize/N-WASP-Antibody-5F4-Immunohistochemistry-Paraffin-H00008976-M04-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] - Immunocytochemical labeling of phosphorylated and unphosphorylated N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP (Ser-484/Ser-485) phospho-specific and N-WASP (Ser-484/Ser-485) unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.](http://images.novusbio.com/fullsize/nbp3-23284_rabbit-n-wasp-p-ser485-p-ser484-pab-19920231992818.jpg "Immunocytochemistry/Immunofluorescence: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] - Immunocytochemical labeling of phosphorylated and unphosphorylated N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP (Ser-484/Ser-485) phospho-specific and N-WASP (Ser-484/Ser-485) unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.")
![Western Blot: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] - Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23284_rabbit-n-wasp-p-ser485-p-ser484-pab-19920231410134.jpg)
![Western Blot: N-WASP Antibody [NBP3-23285] - Western blot of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23285_rabbit-n-wasp-pab-19920231856924.jpg "Western Blot: N-WASP Antibody [NBP3-23285] - Western blot of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP3-23285] - Immunocytochemical labeling of phospho- and unphospho-N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP (Ser-484/Ser-485) phospho-specific and N-WASP (Ser-484/Ser-485) unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of blocking peptide.](http://images.novusbio.com/fullsize/nbp3-23285_rabbit-n-wasp-pab-1992023191544.jpg)
![Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP3-23275] - Immunocytochemical labeling of N-WASP in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal N-WASP/WASP (NBP3-23275) or rabbit polyclonal N-WASP (Tyr-256) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23275_rabbit-n-wasp-pab-199202312403710.jpg "Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP3-23275] - Immunocytochemical labeling of N-WASP in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal N-WASP/WASP (NBP3-23275) or rabbit polyclonal N-WASP (Tyr-256) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: N-WASP Antibody [NBP3-23275] - Western blot analysis of Jurkat (lane 1), A431 (lane 2), and HeLa (lane 3) cell lysates (20 ug/lane). Blots were probed with rabbit polyclonal anti-WASP/N-WASP (NBP3-23275).](http://images.novusbio.com/fullsize/nbp3-23275_rabbit-n-wasp-pab-199202312523623.jpg)
![Western Blot: N-WASP [p Tyr256] Antibody [NBP3-23280] - Western blot analysis of control and pervanadate-treated A431 cells (20 ug/lane). Blots were probed with either rabbit polyclonal anti-N-WASP (Ser-484/Ser-485) or anti-N-WASP (Tyr-256).](http://images.novusbio.com/fullsize/nbp3-23280_rabbit-n-wasp-p-tyr256-pab-19920231240379.jpg "Western Blot: N-WASP [p Tyr256] Antibody [NBP3-23280] - Western blot analysis of control and pervanadate-treated A431 cells (20 ug/lane). Blots were probed with either rabbit polyclonal anti-N-WASP (Ser-484/Ser-485) or anti-N-WASP (Tyr-256).")
![Immunocytochemistry/Immunofluorescence: N-WASP [p Tyr256] Antibody [NBP3-23280] - Immunocytochemical labeling of N-WASP in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal N-WASP/WASP (NBP3-23275) or rabbit polyclonal N-WASP (Tyr-256) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23280_rabbit-n-wasp-p-tyr256-pab-19920231252367.jpg)
![Western Blot: N-WASP Antibody [NBP3-23279] - Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23279_rabbit-n-wasp-pab-1992023141016.jpg "Western Blot: N-WASP Antibody [NBP3-23279] - Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).")
![Western Blot: N-WASP [p Tyr256] Antibody [NB600-1169] - Western blot analysis of control and pervanadate-treated A431 cells (20 ug/lane). Blots were probed with either rabbit polyclonal anti-N-WASP (Ser484/Ser485) or anti-N-WASP (Tyr256).](http://images.novusbio.com/fullsize/N-WASP-[p-Tyr256]-Antibody-Western-Blot-NB600-1169-img0003.jpg "Western Blot: N-WASP [p Tyr256] Antibody [NB600-1169] - Western blot analysis of control and pervanadate-treated A431 cells (20 ug/lane). Blots were probed with either rabbit polyclonal anti-N-WASP (Ser484/Ser485) or anti-N-WASP (Tyr256).")
![Western Blot: N-WASP [p Ser484, p Ser485] Antibody [NBP2-29669] - Western blot of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 and 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 and 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 and 6).](http://images.novusbio.com/fullsize/N-WASP-[p-Ser484--p-Ser485]-Antibody-Western-Blot-NBP2-29669-img0002.jpg "Western Blot: N-WASP [p Ser484, p Ser485] Antibody [NBP2-29669] - Western blot of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 and 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 and 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 and 6).")