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![Western Blot: SMC4 Antibody [NBP1-86635] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NBP1-86635-img0007.jpg "Western Blot: SMC4 Antibody [NBP1-86635] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86635-img0008.jpg)
![Immunohistochemistry-Paraffin: SMC4 Antibody [NBP1-86635] - Staining of human kidney shows strong cytoplasmic positivity with granular pattern in tubule cells.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunohistochemistry-Paraffin-NBP1-86635-img0002.jpg)
![Western Blot: SMC4 Antibody [NBP1-86635] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-544](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NBP1-86635-img0006.jpg)
![Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0002.jpg "Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.")
![Flow Cytometry: SMC4 Antibody [NB100-56394] - Western blot analysis of CAP-C in Ramos cell lysate using NB100-56394 at 2 ug/ml.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0001.jpg)
![Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-375-img0005.jpg "Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.")
![Western Blot: SMC4 Antibody (9Z8S3) [NBP3-16219] - Western blot analysis of extracts of various cell lines, using SMC4 Rabbit mAb (NBP3-16219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMC4-Antibody-9Z8S3-Western-Blot-NBP3-16219-img0004.jpg "Western Blot: SMC4 Antibody (9Z8S3) [NBP3-16219] - Western blot analysis of extracts of various cell lines, using SMC4 Rabbit mAb (NBP3-16219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] - Immunofluorescence analysis of U-2 OS cells using SMC4 Rabbit mAb (NBP3-16219) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC4-Antibody-9Z8S3-Immunocytochemistry-Immunofluorescence-NBP3-16219-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] - Immunofluorescence analysis of C6 cells using SMC4 Rabbit mAb (NBP3-16219) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC4-Antibody-9Z8S3-Immunocytochemistry-Immunofluorescence-NBP3-16219-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] - Immunofluorescence analysis of NIH-3T3 cells using SMC4 Rabbit mAb (NBP3-16219) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC4-Antibody-9Z8S3-Immunocytochemistry-Immunofluorescence-NBP3-16219-img0002.jpg)
![Western Blot: SMC4 Antibody [NB100-374] - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody NB100-374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-374-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC4 Antibody [NB100-374] - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody NB100-374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NB100-374] - HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody used at 5 ug/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-374-img0002.jpg)
![Western Blot: SMC4 Antibody [NB100-374] - Whole cell lysate (90 ug) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibody used at 0.2 ug/ml and goat anti-rabbit HRP used at a dilution of 1:20,000.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-374-img0003.jpg)
![Western Blot: SMC4 Antibody (SR1231) [NBP3-22108] - Western blot analysis using NBP3-22108 in Ramos cell lysate.](http://images.novusbio.com/fullsize/nbp3-22108_rabbit-smc4-mab-sr1231-57202315584816.jpg "Western Blot: SMC4 Antibody (SR1231) [NBP3-22108] - Western blot analysis using NBP3-22108 in Ramos cell lysate.")
![Immunohistochemistry-Paraffin: SMC4 Antibody [NBP2-97937] - Immunochemical staining of human SMC4 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunohistochemistry-Paraffin-NBP2-97937-img0001.jpg "Immunohistochemistry-Paraffin: SMC4 Antibody [NBP2-97937] - Immunochemical staining of human SMC4 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.")